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Radiation-sensitive gene markers and their application in different types of radiation dose monitoring

A gene marker, radiation-sensitive technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve the effect of convenient risk assessment

Active Publication Date: 2022-06-17
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Early and accurate detection of composite rays is the key to emergency management and treatment of nuclear accidents, but it is also a difficult point

Method used

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  • Radiation-sensitive gene markers and their application in different types of radiation dose monitoring
  • Radiation-sensitive gene markers and their application in different types of radiation dose monitoring
  • Radiation-sensitive gene markers and their application in different types of radiation dose monitoring

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Screening and acquisition of radiation-sensitive gene markers

[0051] 1. Irradiation and grouping of animal experiments: 8-10 weeks old C57BL6 female mice were used, SPF level rearing, the rearing conditions were, temperature (23±1) ℃, relative humidity 55%±5%, pressure difference ≤10Pa, daily Light for 12h, free access to food and water.

[0052] 2. After induction of anesthesia with isoflurane before irradiation, it was transferred to a dedicated mouse chest irradiation device and properly fixed. Whole lung field irradiation was performed using heavy ion (carbon-ions, carbon-ions) rays and X-rays. As the main representative of high LET rays, heavy ion rays are carbon particle beams (energy 280MeV) that are accelerated and drained by a cyclotron (20-30 meters in diameter), with extremely high energy and speed. X-ray is one of the representatives of low LET rays, which is irradiated after being accelerated by a medical linear accelerator (energy 6-10MV).

...

Embodiment 2

[0078] This example provides the application of the radiation-sensitive gene marker obtained in Example 1 above in the monitoring of radiation doses of different types of rays. (The application of this embodiment only lists heavy ion rays for illustration, which does not mean that the radiation-sensitive gene marker is not suitable for low-LET rays such as X-rays, and the patent scope of the present invention cannot be limited only by this embodiment)

[0079] see Figure 11 , according to the expression levels of Mgmt, Bax, Thyn1, Phlda3 four genes at different doses, the nonlinear regression model was modeled, and the irradiated heavy ion dose can be indicated according to the average value of the gene combination. According to the gene chip results, the average value of up-regulated and down-regulated gene transcription level changes (log2FC) was taken as the ordinate (that is, y represents the average value of the transcription level (log2FC) of the selected gene marker); ...

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Abstract

The invention provides a radiation-sensitive gene marker and its application in different types of radiation dose monitoring. The radiosensitive gene markers include Mgmt, Bax, Thyn1 and Phlda3. The dose-response curve constructed based on the expression level of the radiation-sensitive gene marker can effectively determine the range of the radiation dose, so as to effectively monitor the dose of different types of radiation exposure, and then accurately indicate the different types of radiation exposure based on biological effects. Reference dose for nuclear radiation damage assessment.

Description

technical field [0001] The invention belongs to the technical field of radiation damage biological dose detection, and in particular relates to a radiation sensitive gene marker and its application in radiation dose monitoring of different types of rays. Background technique [0002] The effect of nuclear radiation on living organisms is an important research content in the field of radiation protection. Militarily, the risk of nuclear tests by neighboring countries, precision nuclear strikes in the context of modern warfare, has not disappeared. The dark cloud of terrorism is rising all over the world, and the use of unknown radioactive substances to target special targets has greater lethality and concealment than conventional weapons, such as densely populated places (such as airports, stations, transportation hubs, etc.), key Location (such as important conference venues, international competition venues, key traffic routes, etc.). In addition, the application of nucle...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
Inventor 周平坤周成周兆明刘晓丹
Owner ACADEMY OF MILITARY MEDICAL SCI
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