79 results about "Downregulated gene" patented technology

The present application provides novel human genes WDRPUH and KRZFPUH, and PPIL1 whose expression is markedly elevated in a great majority of HCCs and colorectal cancers, respectively, compared to corresponding non-cancerous tissues, as well as novel human gene APCDD1 whose expression is elevated in primary colon cancers and down-regulated in response to the transduction of wild-type APC1 into colon-cancer cells. The genes and polypeptides encoded by the genes can be used, for example, in the diagnosis of a cell proliferative disease, and as target molecules for developing drugs against the disease.

The invention discloses a method for detecting cadmium pollution through variation of genetic expression of a brassica plant. The method comprises steps as follows: extracting total RNA (Ribose Nucleic Acid) from a cadmium-processed brassicajuncea root and a compared brassicajuncea root; hybridizing with a 29K arabidopsis chip, so as to obtain a cadmium induced up-regulated gene and a cadmium induced down-regulated gene under specific expression; designing primers in conserved regions of genes; carrying out a semiquantitative PCR (Polymerase Chain Reaction) method to sieve and determine six up-regulated genes and six down-regulated genes with the specific expression of brassicajuncea when cadmium concentration is more than 1.00mg / kg based on a standard of increasing or reducing 2.00 timesof expression quantity; utilizing variation of gene expression in the root of the brassica plant in primer detection environments of 12 genes; if luminance of a product of the brassica plant in the environment to be detected and amplified by the primers of 6 up-regulated genes is increased over 2.00 times that of contrast, and the luminance of the product of the brassica plant in the environment to be detected and amplified by the primers of 6 down-regulated genes is decreased over 2.00 times that of the contrast, determining that concentration of cadmium in the environment to be detected is more than 1.00mg / kg, thus determining that whether the brassica plant in the environment to be detected is subjected to the cadmium pollution. The detection method is high in sensitivity and high in specificity, and can be used for quickly detecting whether content of the cadmium in the environment of the brassica plant is suitable for agricultural production.