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Method for analyzing caenorhabditis elegans head swing inhibition rate of pollutants

A technique of Caenorhabditis elegans and analysis method, which is applied in the direction of analysis of materials, biological tests, material inspection products, etc., can solve the problem of no correlation analysis between the exposure group and the blank control group, and achieve reliable experimental results

Inactive Publication Date: 2020-10-20
TONGJI UNIV
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Problems solved by technology

Although many scholars have studied the neurotoxicity of Caenorhabditis elegans, the methods and results used are limited by the experimental conditions and other reasons, and only a simple study on the number of head swings of Caenorhabditis elegans under exposure to a single or a few pollutant concentrations Statistics, there is no reliable and scientific mathematical model for correlation analysis between the exposure group and the blank control group

Method used

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  • Method for analyzing caenorhabditis elegans head swing inhibition rate of pollutants
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  • Method for analyzing caenorhabditis elegans head swing inhibition rate of pollutants

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Embodiment 1

[0034] Example 1: According to the above method, the toxicity data of chlorpyrifos on the head swing inhibition of Caenorhabditis elegans was determined. The Weibull function in the APTox software was selected to fit the data to obtain the fitting parameters α=14.97, β=2.23, the square of the correlation coefficient was 0.9660, and the root mean square error was 0.0454. And the half effect concentration EC is calculated by the inverse function 50 The value is 1.32629E-7mol·L-1, the upper limit of 95% observational confidence is 1.86897E-7, and the lower limit of 95% observational confidence is 8.92756E-8. Chlorpyrifos dose-response curve figure 2 shown.

Embodiment 2

[0035] Embodiment 2: According to the method described above, the detection method for determining the inhibition rate of triazophos on the head swing of Caenorhabditis elegans. The dilution factor for this example is 0.78328. The Weibull function in the APTox software was selected to fit the data to obtain the fitting parameters α=12.380, β=1.880, the square of the correlation coefficient was 0.9507, and the root mean square error was 0.0584. And the half effect concentration EC is calculated by the inverse function 50 The value is 1.65936E-7mol·L-1, the upper limit of 95% observational confidence is 2.83480E-7, and the lower limit of 95% observational confidence is 8.93650E-8. Triazophos dose-response curve image 3 shown.

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Abstract

The invention discloses a method for analyzing the caenorhabditis elegans head swing inhibition rate of pollutants. A plurality of concentration gradients are set to perform high-flux toxicity exposure on caenorhabditis elegans respectively, toxicity data of a plurality of concentration points are acquired by setting a plurality of parallel groups and repeatedly measuring the toxicity of the samepollutant for multiple times, and a dose-effect curve of a target pollutant is fitted. The method mainly comprises the following steps: preparing a caenorhabditis elegans growth medium; preparing an escherichia coli OP50 culture medium; obtaining a synchronized caenorhabditis elegans stock solution; obtaining environmental pollutant sample solutions with different concentration gradients; carryingout a caenorhabditis elegans toxicity exposure experiment and acquiring toxicity data; and fitting data. According to the method, the neurotoxicity of the environmental pollutants to caenorhabditis elegans can be accurately calculated, and a more scientific theoretical support is provided for evaluating the safety of the environmental pollutants to an ecological system and human health.

Description

technical field [0001] The invention relates to the technical field of environmental pollutant toxicity detection, in particular to an analysis method for the inhibitory rate of pollutants on the head swing of Caenorhabditis elegans. Background technique [0002] With the rapid development of industrialization, more and more compounds have been developed, resulting in a variety of new pollutants in the environment, among which environmental pollutants such as pesticides, heavy metals, antibiotics and other organic compounds are particularly polluting. protrude. Bring huge potential risks to the environment and human health. Therefore, more and more researches are focused on the detection of the toxicity of environmental pollutants. [0003] Physical and chemical analysis and biological detection are usually used in environmental monitoring. Among them, physical and chemical analysis is to quickly and sensitively analyze and determine the type and concentration of pollutan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5085
Inventor 黄朋刘树深王宇
Owner TONGJI UNIV
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