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Radiosensitive gene marker and application thereof in X-ray radiation dose monitoring

A gene marker, radiation-sensitive technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve the effect of convenient risk assessment

Active Publication Date: 2019-06-21
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These biomarkers used for detection are often derived from radiation-related markers or hot research genes reported in the literature, which limits the research and application of related biological dosimeters. Genetic markers that improve the specificity and sensitivity of biological dosimetry assays

Method used

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  • Radiosensitive gene marker and application thereof in X-ray radiation dose monitoring
  • Radiosensitive gene marker and application thereof in X-ray radiation dose monitoring
  • Radiosensitive gene marker and application thereof in X-ray radiation dose monitoring

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Screening and acquisition of radiosensitive gene markers

[0051] 1. Irradiation and grouping of animal experiments: C57BL6 female mice aged 8-10 weeks were used and raised at SPF level. The feeding conditions were: temperature (23±1)°C, relative humidity 55%±5%, pressure difference ≤10Pa, daily Light for 12h, free access to food and water.

[0052] 2. The mice were anesthetized with isoflurane induction and ketamine, fixed properly, and given 10Gy and 20Gy whole-lung field X-ray linear accelerator irradiation respectively, and the blank control group did not receive X-ray irradiation (0Gy). 12 mice were randomly assigned to each group of the control group and each dose of the experimental group. The mice were sacrificed by neck dislocation 24 weeks after irradiation, and fresh lung tissues were taken. The lung tissues were treated with TRIzol and stored at -20°C for future use.

[0053] 3. RNeasy Mini Kit (Qiagen) was used for lung RNA extraction: DNase I (...

Embodiment 2

[0070] This embodiment provides the application of the radiation-sensitive gene markers obtained in the above-mentioned embodiment 1 in X-ray radiation dose monitoring.

[0071] 1. In this embodiment, 8-week-old C57BL6 female mice were used, and a total of 48 mice were divided into three groups, 6 mice did not receive X-ray irradiation (blank group), and 6 mice received 5 times of 2Gy X-ray irradiation (10Gy group), 6 mice received 5 times of 3Gy X-ray irradiation (15Gy group), 6 mice received 5 times of 4Gy X-ray irradiation (20Gy group), 12 mice received 5 times of 6Gy X-ray Irradiation (30Gy group), 12 mice received 5 times of 8Gy X-ray irradiation (40Gy group).

[0072] The mice were anesthetized with isoflurane induction and ketamine, and fixed properly. The mice in the 10Gy group, 15Gy group, 20Gy group, 30Gy group, and 40Gy group were irradiated with lung X-rays according to the set dose, and the mice in the blank group were not irradiated after being fixed. radiation ...

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Abstract

The invention provides a radiosensitive gene marker and application thereof in X-ray radiation dose monitoring. The radiosensitive gene marker comprises three up-regulation gene markers and two down-regulation gene markers; the up-regulation gene markers include Phlda3, Fgg and Kng1; the down-regulation gene markers include Ptprb and Kti. A dose-effect curve constructed based on the expression level of the radiosensitive gene marker helps determine the range of radiation dose effectively, so that the level of X-ray radiation dose can be monitored effectively to accurately prompt a reference dose of X-ray radiation based on biological effect in order to estimate nuclear radiation damage.

Description

technical field [0001] The invention belongs to the technical field of radiation damage biological dose detection, and in particular relates to a radiation-sensitive gene marker and its application in X-ray radiation dose monitoring. Background technique [0002] X-ray is a kind of ionizing radiation widely used in clinical tumor radiotherapy. It has biological effects such as strong penetrating effect, fluorescence effect, genomic DNA damage and cell killing. These characteristics make X-ray play a role in industrial and biomedical fields. played an important role. X-rays have been widely used in industrial, manufacturing, transportation, and military fields such as industrial exploration, weapon armor, and metal quality inspection. X-rays also have extremely important diagnostic and therapeutic value in medicine, including computed tomography (Computed Tomography, CT) examination, digital radiography (Digital Radiography, DR) or digital subtraction angiography (Digital Su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
Inventor 周平坤周兆明周成刘晓丹
Owner ACADEMY OF MILITARY MEDICAL SCI
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