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33 results about "Cordyceps guangdongensis" patented technology

Large-scale cultivation method for Guangdong fruiting bodies of cordyceps military

The invention relates to the technical field of cultivation of fruiting bodies of cordyceps military, in particular to a large-scale cultivation method for Guangdong fruiting bodies of cordyceps military. The large-scale cultivation method includes the steps: A, distribution; B, preparation of strains including B1, slope strains, B2, shake flask strains and B3 fermented strains; C, inoculation and mycelium culture; D, fruiting body culture; and E, recovery. The deep fermented strains replace existing multistage shake flask strains and are high in hyphae vigor, mycelium pellets are fine in size uniformity, inoculation can be performed by an automatic inoculation machine or manual spraying in a sterile environment, assembly line operation is realized, inoculation amount is stable, each bottle is 40-60mL, spraying is uniform, hyphae rapidly sprout, contamination rate is reduced, fruiting body coming time is 3-5 days shorter than that of the prior art, the whole production cycle is shortened by about 10 days, and labor and raw material costs can be saved as compared with shake flask strain inoculation in the prior art.
Owner:韶关市星河生物科技有限公司 +2

Total-nutrient formula food for inflammatory bowel disease patients

The invention provides total-nutrient formula food for inflammatory bowel disease patients. According to the food, selenium-rich plant protein powder serves as a main nutritional component, various common Chinese herbal medicines with the function of adjusting intestine and stomach are added, papaws, green tangerine peel and cordyceps militaris are added and have the functions of clearing toxic substances in the body and improving body immunity, tempeh and cordyceps guangdongensis are added and have quite good functions of resisting and inhibiting bacteria and particularly have remarkable inhibiting effects on food spoilage bacteria and pathogenic bacteria, mannan-oligosaccharide is added, so that intestinal absorption is facilitated, and the mannan-oligosaccharide has remarkable effect on middle-aged and aged people with poor intestinal absorption. The food is processed into powder according to a conventional method and easily absorbed when being taken for a long time.
Owner:恩施硒域传奇健康管理有限公司

Characteristic nucleotide sequence, nucleic acid molecular probes, kit and method for identifying branch caterpillar fungus

The invention discloses a characteristic nucleotide sequence, nucleic acid molecular probes, a kit and a method for identifying branch caterpillar fungus. The characteristic nucleotide sequence is shown in SEQ ID No.1. The nucleic acid molecular probes are FZF:5'-AGCGGTGGGCGAATGA-3' and FZR:5'-ACTTTAGCTGCGGGCG-3'. The identifying method is characterized in that an ITS rDNA sequence gene of the branch caterpillar fungus is used as a template, the nucleic acid molecular probes FZF and FZR are used as PCR (polymerase chain reaction) primers, and a conventional PCR method is used for identifying. After proofed by experiments, only the branch caterpillar fungus can be amplified to a special segment, and the other cordyceps samples, such as cordyceps sinensis, cordyceps militaris, cordyceps guangdongensis and cordyceps sobolifera cannot be amplified to any segment, so the nucleic acid molecular probes FZF and FZR have very high specificity, the sequence testing procedure is not needed, and the genuineness of the branch caterpillar fungus (such as mycelium and sporocarp) can be quickly identified.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

Characteristic Nucleotide Sequence, Nucleic Acid Molecular Probe and Method for Identifying Cantonese Cordyceps

The invention discloses a characteristic nucleotide sequence, nucleic acid molecular probes and a method for identifying Cordyceps guangdongensis. The characteristic nucleotide sequence is shown as SEQ ID NO.1. The nucleic acid molecular probes comprise GDF of 5'-CTGTTGGCATCTTCTGAGTCTC-3' and GDR of 5'-GGTGCGAGGTTGTGCTACTA-3'. In the method, the nucleic acid molecular probes of GDF and GDR are used as polymerase chain reaction (PCR) primers, and a PCR method is used for identifying the Cordyceps guangdongensis; and steps are carried out according to a conventional PCR method. The GDF and the GDR are used as primers, the genome DNA of the Cordyceps guangdongensis is taken as a template, PCR is carried out according to the conventional PCR method, and the Cordyceps guangdongensis is amplified to a specific segment. Through experiments, only the Cordyceps guangdongensis can be amplified to the specific segment, while other Cordyceps samples such as Cordyceps sinensis, Cordyceps gunnii, Cordyceps militaris, supergene Cordyceps and Cordyceps brasiliensis are not amplified to any segment, so that the nucleic acid molecular probes of GDF and GDR have high specificity and can be used for quickly identifying truth and false of the Cordyceps guangdongensis (a fruit body and a mycelium) and related products.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

Formula for nourishing and reinforcing kidney and replenishing qi and blood and preparation method

The invention discloses a formula for nourishing and reinforcing the kidney and replenishing qi and blood and a preparation method. The formula for nourishing and reinforcing the kidney and replenishing the qi and the blood comprises the following raw materials: apple condensed juice with a weight-to-volume ratio of 1-10 g / L, fine peptide powder with a weight-to-volume ratio of 1-10 g / L, deer sinew peptide powder with a weight-to-volume ratio of 1-10 g / L, maca aqueous extract powder with a weight-to-volume ratio of 1-10 g / L, fructus lycii powder with a weight-to-volume ratio of 1-10 g / L, donkey-hide gelatin powder with a weight-to-volume ratio of 1-10 g / L, red date powder with a weight-to-volume ratio of 1-10 g / L, rhizoma polygonati powder with a weight-to-volume ratio of 1-10 g / L, mulberry powder with a weight-to-volume ratio of 1-10 g / L, fructus rubi powder with a weight-to-volume ratio of 1-10 g / L, cordyceps guangdongensis fruitbody aqueous extract powder with a weight-to-volume ratio of 1-10 g / L, yam flour with a weight-to-volume ratio of 1-10 g / L, poria powder with a weight-to-volume ratio of 1-10 g / L, ginseng aqueous extract powder with a weight-to-volume ratio of 0.1-1 g / L and saussurea involucrata culture with a weight-to-volume ratio of 0.01-0.1 g / L. According to the formula for nourishing and reinforcing the kidney and replenishing the qi and the blood and the preparation method, traditional Chinese medicine theories of the nation are used, the components are rigorous, the medicine compatibility is reasonable, and the formula for nourishing and reinforcing the kidney and replenishing the qi and the blood has the efficacy of nourishing and reinforcing the kidney, replenishing the qi and the blood, engendering essence and boosting marrow, and can improve a chronic fatigue syndrome fundamentally.
Owner:广东澳思瑞雅健康美容生物科技有限公司

Ginseng cordyceps hericium erinaceus protein powder and preparation method thereof

InactiveCN108936699ADoes not affect useDoes not affect metabolismFood scienceNutritive valuesVitamin C
The present invention discloses a ginseng cordyceps hericium erinaceus protein powder and a preparation method thereof. The protein powder comprises the following components in parts by weight: 8 to 15 parts of ginseng, 4 to 6 parts of Cordyceps guangdongensis fruiting bodies, 4 to 5 parts of cordyceps militaris fruiting bodies, 8 to 9 parts of hericium erinaceus, 15 to 25 parts of soybean proteinpowder, 40 to 50 parts of soybean protein isolate powder, 1 to 3 parts of poria cocos, 1 to 3 parts of Chinese yams, 1 to 3 parts of inner membrane of chicken gizzard, 1 to 3 parts of bone marrow powder, 0.0008 to 0.0010 part of vitamin A, 0.1 to 0.3 part of vitamin C, 5 to 7 parts of sorbitol and 0.2 to 0.4 part of non-dairy creamer. The ginseng cordyceps hericium erinaceus protein powder can beused for daily eating, can ingest a plurality of substances beneficial to the body when in use, does not influence protein utilization and metabolism as the ingestion amount is within the permitted range, and has high nutritive value. The preparation method has simple operation, short period and uniform mixing.
Owner:安徽三九华康药业有限公司

Cordyceps guangdongensis culture medium

The invention provides a cordyceps guangdongensis culture medium. The culture medium is composed of grains and a nutrient solution according to a weight-to-volume ratio g / mL ranging from 1:1 to 1:2; and the nutrient solution is composed of the following components in percentage by weight: 1%-3% of saccharose or D-mannitol, 0.1%-1% of a yeast extract, 0.1%-2% of potassium chloride, 0.1%-1% of soybean meal and the balance water.
Owner:浙江泛亚保健食品有限公司

Traditional Chinese medicine composition with homology of medicine and food for invigorating kidney and strengthening Yang and preparation method thereof

The invention relates to a traditional Chinese medicine composition with homology of medicine and food for invigorating the kidney and strengthening Yang and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials and components in parts by mass: 20 to 60 parts of fructus lycii, 5 to 20 parts of herba cistanche, 10 to 20 parts of semen phaseoli, 2 to 10 parts of platycodonis radix, 5 to 10 parts of Guangdong cordyceps sinensis sporocarp, 3 to 5 parts of ginseng radix et rhizome, 3 to 6 parts of cordyceps militaris, 2 to 10 parts of semen myristicae, 1 to 2 parts of maca, 5 to 15 parts of honeysuckle flower, and the like. The traditional Chinese medicine composition for invigorating the kidney and strengthening Yang has the advantages that the composition is extracted from the plants with homology of medicine and food; the formula and proportional ratio are scientific, any artificial synthetic component is not contained, the safety is realized, and any toxic or side effect is avoided; the excellent effects of invigorating the kidney and strengthening Yang, enhancing body and resisting aging, enhancing blood circulation,and enhancing immunity of the human body are realized, the sub-health states of the human body, such as kidney deficiency, can be effectively relieved, and the working efficiency and life quality ofpeople are improved; the effect is obvious, the convenience in use is realized, and the wide application value is realized.
Owner:河南五极中医药研究院

Characteristic nucleotide sequence, nucleic acid molecule primers and method for quantitative determination of cordyceps guangdongensis

The invention discloses a characteristic nucleotide sequence, nucleic acid molecule primers and a method for quantitative determination of cordyceps guangdongensis. The characteristic nucleotide sequence for quantitative determination of cordyceps guangdongensis is as shown in SEQ ID NO. 2. The nucleic acid molecule primers for quantitative determination of cordyceps guangdongensis are shown as follows: FS: 5'-GCTCCGATTCGATGGC-3'; RS: 5'-TACAGCAGGCAGGTGGC-3'. When the nucleic acid molecule primers FS and RS of the invention are applied to fluorescence PCR amplification, a fluorescence signal is excited only in cordyceps guangdongensis, while other cordyceps such as cordyceps sinensis, cordyceps militaris and ramous cordyceps are free from Ct value when the number of cycles is less than 35, showing that the nucleic acid molecule primers FS and RS of the invention are quite high in specificity and are suitable for rapid adulteration and content detection of cordyceps guangdongensis (mycelium and fruiting body) and related products thereof.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

Medical and edible nerve-calming sleep-assisting Chinese medicinal composition and preparation method thereof

The invention relates to a medical and edible nerve-calming sleep-assisting Chinese medicinal composition and a preparation method thereof. Raw material components comprise: 30 to 60 weight parts of lily bulbs, 3 to 6 weight parts of fruiting bodies of Guangdong cordyceps militaries, 10 to 30 weight parts of donkey-hide gelatin, 10 to 30 weight parts of inulin, 2 to 10 weight parts of folium cortex eucommiae, 3 to 6 weight parts of spirulina, 2 to 5 weight parts of cordyceps militaris, 5 to 20 weight parts of lotus seeds, 5 to 15 weight parts of malt, 1 to 5 weight parts of tomato seed oil, 5to 10 weight parts of moringa leaves, 10 to 30 weight parts of black sesame, 1 to 5 weight parts of fructus gardeniae and the like. The nerve-calming sleep-assisting composition disclosed by the invention is formed by selecting medical and edible plants to extract, is scientific in formula proportion, is not added with any artificially synthesized component, is safe, has no toxic and / or side effects and has excellent effects of invigorating spleen-stomach and replenishing qi, calming nerves and assisting in sleep and relieving neurasthenia, insomnia and forgetfulness; an organism sub-health status caused by insomnia and dreaminess can be effectively relieved; and the working efficiency and the life quality of people are promoted.
Owner:河南五极中医药研究院

Traditional Chinese medicine composition with homology of medicine and food for invigorating spleen and removing dampness and preparation method thereof

The invention relates to a traditional Chinese medicine composition with homology of medicine and food for invigorating the spleen and removing dampness and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials and components in parts by mass: 15 to 30 parts of fructus crataegi, 1 to 2 parts of dried longan pulp, 3 to 6 parts of rhizoma dioscoreae, 1 to 3 parts of herba cistanche, 2 to 5 parts of cordyceps militaris, 3 to 8 parts of fructus ziziphi jujubae, 3 to 6 parts of rose flower, 1 to 3 parts of Guangdong cordyceps sinensis sporocarp, 2 to 5 parts of lily bulb, 5 to 10 parts of fructus corni, 1 to 3 parts of folium cortex eucommiae, 0.5 to 2 parts of barley, 1 to 3 parts of herba menthae haplocalycis, 2 to 5 parts of radix codcnopsitis pilosulas, and 1 to 3 parts of honeysuckle flower. The traditional Chinese medicine composition for invigorating the spleen and removing dampness has the advantages that the composition is extracted from the plants with homology of medicine and food; the formula and proportional ratio are scientific, any artificial synthetic component is not contained, the safety is realized, andany toxic or side effect is avoided; the excellent effects of regulating the spleen and stomach, regulating Qi and regulating the middle warmer, clearing damp and heat, and enhancing immunity of human body are realized, the sub-health state of the human body is effectively relieved, and the working efficiency and life quality of people are improved.
Owner:河南五极中医药研究院

Formula for nourishing and protecting liver, dispelling effects of alcohol and expelling toxin and preparation method

The invention discloses a formula for nourishing and protecting the liver, dispelling effects of alcohol and expelling toxin and a preparation method. The formula for nourishing and protecting the liver, dispelling the effects of the alcohol and expelling the toxin comprises the following raw materials: water as a solvent, corn oligopeptides powder with a weight-to-volume ratio of 50-150 g / L, glycine betaine (a natural extract) with a weight-to-volume ratio of 5-10 g / L, cordyceps guangdongensis fruitbody powder with a weight-to-volume ratio of 1-2 g / L, ginseng extract powder with a weight-to-volume ratio of 1-2 g / L, pueraria extract powder with a weight-to-volume ratio of 1-10 g / L, fructus lycii powder with a weight-to-volume ratio of 1-10 g / L, semen hoveniae with a weight-to-volume ratioof 1-10 g / L, eucommia ulmoides oliv male flower powder with a weight-to-volume ratio of 1-2 g / L and radix curcuma with a weight-to-volume ratio of 0.1-0.5 g / L. The formula for nourishing and protecting the liver, dispelling the effects of the alcohol and expelling the toxin is formed by selecting and extracting medicinal and edible plants, a component ratio is scientific, and no artificially-synthesized components are added, so that the formula for nourishing and protecting the liver, dispelling the effects of the alcohol and expelling the toxin is safe and free from toxin and side effects, and has the efficacy of nourishing and protecting the liver, dispelling the effects of the alcohol, expelling the toxin, repairing liver cells, promoting metabolism, clearing free radicals and improvingthe organism immunity.
Owner:广东澳思瑞雅健康美容生物科技有限公司

Cordyceps guangdongensis polysaccharides CCG-PSW and CCG-PSS, preparation method and application

ActiveCN109021132AClear physical and chemical propertiesClear structureDPPHHydroxyl radical
The invention discloses cordyceps guangdongensis polysaccharides CCG-PSW and CCG-PSS, a preparation method and application. The invention for the first time identifies the structures of cordyceps guangdongensis polysaccharides CCG-PSW and CCG-PSS, makes the physicochemical properties and structures of the two polysaccharide components clear, and further explores the antioxidant activity. The result indicates that: in antioxidant activity experiments, the CCG-PSW and CCG-PSS polysaccharide pure products have the effect of scavenging hydroxyl radicals, DPPH radicals and ABTS radicals, ferrous ion chelating ability and reducing ability, and the action force is concentration-dependent. The cordyceps guangdongensis polysaccharides CCG-PSW and CCG-PSS can be used for preparation of antioxidant cosmetics, food, health products or drugs.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

Cordyceps guangdongensis and hypsizigus marmoreus compound with effect of relieving physical fatigues

The invention relates to a cordyceps guangdongensis and hypsizigus marmoreus compound which is characterized by comprising the following components in mass percentage: 33%-75% of cordyceps guangdongensis powder and 25%-67% of hypsizigus marmoreus powder. The cordyceps guangdongensis and hypsizigus marmoreus compound is low in cost, suitable for all kinds of people and excellent in popularization significance and has excellent effects of relieving physical fatigues, resisting hypoxia, enhancing immunity and improving cardiovascular functions.
Owner:GUANGDONG INST OF MICROORGANISM

Cordyceps guangdongensis wine

The present invention relates to cordyceps guangdongensis wine. In the cordyceps guangdongensis wine, a weight-to-volume ratio (g / ml) of cordyceps guangdongensis to wine is 1:(40-50). The prepared cordyceps guangdongensis wine has sweet taste, strong fragrance and high content of active ingredient polysaccharides, and has functions of significantly improving immunity and delaying aging.
Owner:浙江泛亚保健食品有限公司

Nutrient solution beneficial to post-operation rehabilitation of tumor patients and preparation method of nutrient solution

The invention discloses a nutrient solution beneficial to post-operation rehabilitation of tumor patients and a preparation method of the nutrient solution and relates to the technical field of nutrient solutions for post-operation rehabilitation of the tumor patients. The nutrient solution is prepared from, by weight, 5-15 parts of cordyceps militaris, 3-12 parts of an extract of fruitbodies of cordyceps guangdongensis, 1-2 parts of unsaturated fatty acid, 2-3 parts of water-soluble dietary fibers, 2-10 parts of phylloporia ribis fermentation mycelia, 3-5 parts of globin peptide, 2-4 parts ofacai berries, 3-5 parts of a composite fruit and vegetable enzyme, 8-12 parts of fructo-oligosaccharide and 3-5 parts of a buffer solution. The nutrient solution beneficial to post-operation rehabilitation of the tumor patients is low in production cost and beneficial to industrial production and can effectively meet the market demand.
Owner:深圳市德福康泰生物科技有限公司

Cordyceps guangdongensis protoplast preparation and recovery method

ActiveCN109943490AConducive to later genetic transformation researchFacilitates fusion of protoplastsFungiMicroorganism based processesRecovery methodCordyceps
The invention discloses a cordyceps guangdongensis protoplast preparation and recovery method. The preparation method comprises the steps of mycelium culture, protoplast preparation, protoplast purification and the like, wherein a mixed enzyme system containing lyase and driselase is adopted to enzymolyse a mycelium to obtain a protoplast of cordyceps guangdongensis, and a TB3 recovery culture medium is used for recovering the cordyceps guangdongensis protoplast. The cordyceps guangdongensis protoplast preparation and recovery method has the advantages that a cordyceps guangdongensis protoplast preparation and recovery system is firstly established, thereby being conductive to the later genetic transformation research of the cordyceps guangdongensis or the implementation of protoplast fusion and other technologies, increasing the research speed in the genetics, breeding and other aspects, being conductive to promoting the progress of the research and application of the system in the field of healthcare food and medicine, and bringing benefits to the society.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

Large-scale cultivation method for Guangdong fruiting bodies of cordyceps military

The invention relates to the technical field of cultivation of fruiting bodies of cordyceps military, in particular to a large-scale cultivation method for Guangdong fruiting bodies of cordyceps military. The large-scale cultivation method includes the steps: A, distribution; B, preparation of strains including B1, slope strains, B2, shake flask strains and B3 fermented strains; C, inoculation and mycelium culture; D, fruiting body culture; and E, recovery. The deep fermented strains replace existing multistage shake flask strains and are high in hyphae vigor, mycelium pellets are fine in size uniformity, inoculation can be performed by an automatic inoculation machine or manual spraying in a sterile environment, assembly line operation is realized, inoculation amount is stable, each bottle is 40-60mL, spraying is uniform, hyphae rapidly sprout, contamination rate is reduced, fruiting body coming time is 3-5 days shorter than that of the prior art, the whole production cycle is shortened by about 10 days, and labor and raw material costs can be saved as compared with shake flask strain inoculation in the prior art.
Owner:韶关市星河生物科技有限公司 +2

Application of cordyceps guangdongensis sporocarp polysaccharide in preparation of medicine for treating and improving obesity and related diseases

The invention discloses application of cordyceps guangdongensis sporocarp polysaccharide in preparation of drugs for treating and improving obesity and related diseases. The invention provides application of cordyceps guangdongensis sporocarp polysaccharide in preparation of drugs for treating and improving obesity and related diseases. In the aspects of relieving obesity, reducing blood fat and protecting the liver, the cordyceps guangdongensis sporocarp polysaccharide plays a positive role and shows good development and application prospects, and the application field of the cordyceps guangdongensis sporocarp polysaccharide is greatly expanded.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

Characteristic nucleotide sequence, nucleic acid molecular probe, kit and method for identifying branched Cordyceps

The invention discloses a characteristic nucleotide sequence, nucleic acid molecular probes, a kit and a method for identifying branch caterpillar fungus. The characteristic nucleotide sequence is shown in SEQ ID No.1. The nucleic acid molecular probes are FZF:5'-AGCGGTGGGCGAATGA-3' and FZR:5'-ACTTTAGCTGCGGGCG-3'. The identifying method is characterized in that an ITS rDNA sequence gene of the branch caterpillar fungus is used as a template, the nucleic acid molecular probes FZF and FZR are used as PCR (polymerase chain reaction) primers, and a conventional PCR method is used for identifying. After proofed by experiments, only the branch caterpillar fungus can be amplified to a special segment, and the other cordyceps samples, such as cordyceps sinensis, cordyceps militaris, cordyceps guangdongensis and cordyceps sobolifera cannot be amplified to any segment, so the nucleic acid molecular probes FZF and FZR have very high specificity, the sequence testing procedure is not needed, and the genuineness of the branch caterpillar fungus (such as mycelium and sporocarp) can be quickly identified.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

Characteristic nucleotide sequence, nucleic acid molecular probes and method for identifying Cordyceps guangdongensis

The invention discloses a characteristic nucleotide sequence, nucleic acid molecular probes and a method for identifying Cordyceps guangdongensis. The characteristic nucleotide sequence is shown as SEQ ID NO.1. The nucleic acid molecular probes comprise GDF of 5'-CTGTTGGCATCTTCTGAGTCTC-3' and GDR of 5'-GGTGCGAGGTTGTGCTACTA-3'. In the method, the nucleic acid molecular probes of GDF and GDR are used as polymerase chain reaction (PCR) primers, and a PCR method is used for identifying the Cordyceps guangdongensis; and steps are carried out according to a conventional PCR method. The GDF and the GDR are used as primers, the genome DNA of the Cordyceps guangdongensis is taken as a template, PCR is carried out according to the conventional PCR method, and the Cordyceps guangdongensis is amplified to a specific segment. Through experiments, only the Cordyceps guangdongensis can be amplified to the specific segment, while other Cordyceps samples such as Cordyceps sinensis, Cordyceps gunnii, Cordyceps militaris, supergene Cordyceps and Cordyceps brasiliensis are not amplified to any segment, so that the nucleic acid molecular probes of GDF and GDR have high specificity and can be used for quickly identifying truth and false of the Cordyceps guangdongensis (a fruit body and a mycelium) and related products.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

A kind of preparation and recovery method of Guangdong Cordyceps grass biomass

ActiveCN109943490BSpeed up researchAdvancing research application progressFungiMicroorganism based processesMyceliumLyase
The invention discloses a preparation and recovery method of Guangdong Cordyceps grass biomass. The preparation method includes the steps of mycelium cultivation, protoplast preparation, protoplast purification, etc., using a mixed enzyme system containing lyase and collapse enzyme to enzymolyze the mycelia of Cordyceps guangdong to obtain the protoplasts of Cordyceps guangdong, and Use TB 3 The recovery medium was used to recover the protoplasts of Cordyceps prairie in Guangdong. The present invention establishes the preparation and recovery system of Guangdong Cordyceps protoplasts for the first time, which is beneficial to the implementation of technologies such as late genetic transformation research or protoplast fusion of Cordyceps cantonese, accelerates the research speed of its genetics and breeding, and helps to promote its in health care. The progress of research and application in the field of food and medicine, benefiting society.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

Cordyceps guangdongensis medicinal liquor capable of reducing uric acid and brewing method and application of Cordyceps guangdongensis medicinal liquor

The invention discloses Cordyceps guangdongensis medicinal liquor capable of reducing uric acid as well as a brewing method and an application thereof, and belongs to the technical field of traditional Chinese medicines. According to the present invention, Cordyceps guangdongensis is adopted as the raw material, and the Cordyceps guangdongensis medicinal liquor with the uric acid reducing effect is provided, and is prepared from 15 parts by weight of Cordyceps guangdongensis sporocarp powder, 3 parts by weight of pilose antler blood powder and 1500 parts by weight of Baijiu. The brewing method putting the cordyceps guangdongensis sporocarp powder and the pilose antler blood powder into a disinfected container, adding the white spirit, sealing the container, and storing for one month for taking. The animal experiment results show that the uric acid reducing effect of the product is close to that of a clinical medicine allopurinol tablet. The medicinal liquor raw material formula has good development and utilization prospects.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1

A high-yield and high-quality cultivated strain of Cordyceps guangdong and its identification primers and identification methods

The invention discloses a high-yield and high-quality cultivated strain of Cordyceps guangdong and its identification primers and identification method. The high-yield and high-quality Cordyceps guangdong is strain GDGM 30035, and the preservation number is: GDMCC No: 61208. The strain has the characteristics of short growth cycle and good fruiting body properties, and is suitable for large-scale promotion. The present invention also provides a kind of identification primer and identification method for identifying the above-mentioned Cordyceps guangdong cultivated strain GDGM 30035, which can be used to accurately identify the cultivated strain GDGM 30035 of Cordyceps guangdong, avoiding any comparison with other types of Cordyceps or production Confusion with other Cordyceps from Guangdong prevents consumers from misleading consumers by using other types of Cordyceps or other Cordyceps produced in Guangdong as Cantonese Cordyceps, so as to protect the rights and interests of consumers.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

Culture method for cordyceps guangdongensis T.H.Li,Q.Y.Lin&B.Song

The invention discloses a culture method for cordyceps guangdongensis T.H.Li,Q.Y.Lin&B.Song. The method comprises the processes of liquid culture, solid culture and harvest, wherein the solid culture process comprises the following steps of a, preparing a solid culture medium, specifically, preparing the solid culture medium according to a material-water ratio of 1:(1-1.2); b, carrying out inoculation, specifically, inoculating the solid culture medium with a seed solution obtained via liquid culture; and c, carrying out solid culture.
Owner:ZHEJIANG BIOASIA PHARMA CO LTD

High-yield and high-quality Tolypocladium guangdongense cultivation strain as well as identification primer and identification method thereof

The invention discloses a high-yield and high-quality Tolypocladium guangdongense cultivation strain as well as an identification primer and an identification method thereof. The high-yield and high-quality Tolypocladium guangdongense is a bacterial strain GDGM 30035, and the preservation number is GDMCC No: 61208. The strain has the characteristics of short growth period, good sporocarp characterand the like, and is suitable for large-area popularization. The invention also provides an identification primer and an identification method for identifying the Tolypocladium guangdongense cultivation strain GDGM 30035, and the Tolypocladium guangdongense cultivation strain GDGM 30035 can be accurately identified by adopting the identification primer and the identification method, so that the Tolypocladium guangdongense cultivation strain GDGM 30035 is prevented from being mixed with other types of cordyceps sinensis or other cordycepssinensis (mainly are cordyceps militaris) produced fromGuangdong; and misleading of consumers due to market behaviors of pretending Tolypocladium guangdongense by utilizing other types of cordyceps sinensis or other cordyceps sinensis (mainly are cordyceps militaris) produced from Guangdong is avoided, so that rights and interests of the consumers are guaranteed.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

Application of ethanol extract from fruiting bodies of Cordyceps guangdong in the preparation of drugs for preventing obesity and hyperlipidemia

The invention discloses the application of the fruiting body ethanol extract of Cordyceps guangdong in the preparation of medicines for preventing obesity and hyperlipidemia. The present invention finds for the first time that the ethanol extract of Cordyceps guangdong can alleviate the weight gain by reducing the fat content in the groin, epididymis and liver of obese mice with a high-fat diet; reduce serum triglyceride, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol. Density lipoprotein cholesterol content, thereby relieving the symptoms of hyperlipidemia. The fruiting body of Cordyceps guangdong has played an active and effective role in relieving obesity and hypolipidemic symptoms, has a good application prospect, and greatly expands its application field.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

A high-yield and high-quality cultivated strain gdgm 60 of Guangdong Cordyceps

The invention discloses a high-yield and high-quality cultivated strain of Cordyceps guangdongensis GDGM 60, and the preservation number is GDMCC No: 61278. The strain is screened and obtained by the following method: UV-induced mycelium of Cordyceps guangdong to produce conidia, and after isolation of single spores, high-yield and high-quality strains are screened through liquid shake flask culture, plate primordia induction and fruiting body development cultivation. The mycelium of this strain is white on PDA medium, and the mycelium grows faster in YMPT liquid medium, and light induces the rapid formation of primordia, and the color of mature fruiting body is light green or light yellow, which are significantly increased. Entity length and diameter averaged 8.9 cm and 2.6 mm. During large-scale cultivation, mycelium fermentation time is shortened by 10-15%; the time from primordium formation to fruiting body maturity is shortened by about 7-10%; fruiting body length is increased by about 19%; fruiting body diameter is increased by about 66%; Entity quality improved significantly.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

High-yield and high-quality cultivation strain GDGM 60 of cordyceps guangdongensis

The invention discloses a high-yield and high-quality cultivation strain GDGM 60 of cordyceps guangdongensis. The preservation number of the high-yield and high-quality cultivation strain GDGM 60 is GDMCC No: 61278. The strain is screened by the following method: inducing Guangdong cordyceps mycelia to generate conidia by ultraviolet, separating single spores, and screening the high-yield high-quality strain by liquid shake-flask culture, plate primordium induction and sporocarp development and cultivation. The hypha of the strain is white on a PDA culture medium, the hypha in a YMPT liquid culture medium grows faster, an illumination-induced primordium is rapidly formed, the color of a mature sporocarp is light green or light yellow and is obviously increased, and after 40 days of illumination culture, the average length and diameter of the sporocarp are 8.9 cm and 2.6 mm. During large-scale cultivation, the mycelium fermentation time is shortened by 10-15%; the time from primordium formation to sporocarp maturation is shortened by about 7-10%; the sporocarp length is increased by about 19%; the sporocarp diameter is increased by about 66%; the salient improvement of sporocarp quality is achieved.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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