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A high-yield and high-quality cultivated strain gdgm 60 of Guangdong Cordyceps

A technology of GDGM60 and Cordyceps guangdong, which is applied in the fields of cultivation, plant cultivation, mushroom cultivation, etc., and can solve the problems of further improvement in quality and yield

Active Publication Date: 2022-02-11
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the traits of the strains currently used for cultivation are good and the yield is relatively stable, their quality and yield still need to be further improved.

Method used

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  • A high-yield and high-quality cultivated strain gdgm 60 of Guangdong Cordyceps
  • A high-yield and high-quality cultivated strain gdgm 60 of Guangdong Cordyceps
  • A high-yield and high-quality cultivated strain gdgm 60 of Guangdong Cordyceps

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: Cordyceps guangdong monospore isolated strain obtains

[0049] Inoculate Guangdong Cordyceps standard strain GDGM 30035 into solid PDA medium (i.e. potato dextrose agar medium, the preparation method is to weigh 200g potatoes, wash, peel and chop them, add 1000mL of water to boil for half an hour, filter with gauze, and add 20g Glucose and 17g of agar, fully dissolved, filtered with hot gauze, put the filtrate on 121°C for about 20min, and cool it to get it), put it on 23±1°C for dark culture for 30d, the mycelium grows to about 90cm for cultivation 3 / 4 of the edge of the dish. Put the mycelium under the ultraviolet light for 30 minutes, then turn it to dark culture at 23±1°C, add appropriate amount of sterile water to the petri dish after spore production, gently scrape the medium with the tip of the pipette, so that the spores are scattered Filter the bacterial water with four layers of sterile gauze, collect the filtrate, centrifuge at 12000rpm / min for...

Embodiment 2

[0050] Example 2: Screening of Cordyceps guangdong high-yield and high-quality strains

[0051] Inoculate a single colony strain on a solid PDA plate and culture it in the dark at 23±1°C. When the colony grows to 2 / 3 of the culture dish, switch to 10L / 14D periodic irradiation under 500Lux light conditions to induce the formation of primordium, and observe the formation speed of primordium. The single-spore isolated strains capable of rapidly inducing primordia on the screening plate were obtained, and 100 single-spore isolated strains were obtained, and the single-spore isolated strains were inoculated into YMPT liquid medium (its formula is that every liter of medium contains 6 g of malt extract, pancreatic 10g of peptone, 20g of glucose, 6g of yeast extract, the solvent is deionized water; it is prepared by mixing the components in the medium with deionized water, stirring and dissolving, sterilizing at 121°C for 10min, and cooling to obtain), 120rpm / min shake flask cultur...

Embodiment 3

[0053] Example 3: Molecular biological characteristics of high-yield and high-quality strain GDGM 60 of Cordyceps guangdong and its application in large-scale cultivation

[0054] The fruiting body of Cordyceps guangdong GDGM 60 strain grows upwards, uniformly narrowed, columnar or rod-shaped, light green to light yellow ( figure 2 ), after 40 days of light, the length of the fruiting body was 6.9-11.9 cm, an average of 8.9 cm, an increase of 19% compared with the original strain GDGM 30035; the diameter of the fruiting body was 1.8-4.2 mm, with an average of 2.6 mm, an increase of 66 %. The ratio of its fruiting body diameter to length is 0.03, which is 50% higher than that of the original strain GDGM 30035 (Table 1), and the liquid fermentation culture mycelium fermentation time has been shortened by 10% to 15%; About 7% to 10% shortened; fruit body fresh state and after drying the appearance shape and color are better than the original strain ( figure 2 and image 3 ), t...

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Abstract

The invention discloses a high-yield and high-quality cultivated strain of Cordyceps guangdongensis GDGM 60, and the preservation number is GDMCC No: 61278. The strain is screened and obtained by the following method: UV-induced mycelium of Cordyceps guangdong to produce conidia, and after isolation of single spores, high-yield and high-quality strains are screened through liquid shake flask culture, plate primordia induction and fruiting body development cultivation. The mycelium of this strain is white on PDA medium, and the mycelium grows faster in YMPT liquid medium, and light induces the rapid formation of primordia, and the color of mature fruiting body is light green or light yellow, which are significantly increased. Entity length and diameter averaged 8.9 cm and 2.6 mm. During large-scale cultivation, mycelium fermentation time is shortened by 10-15%; the time from primordium formation to fruiting body maturity is shortened by about 7-10%; fruiting body length is increased by about 19%; fruiting body diameter is increased by about 66%; Entity quality improved significantly.

Description

technical field [0001] The invention relates to the technical field of edible fungi, in particular to a high-yield and high-quality cultured strain of Cordyceps guangdong GDGM60. Background technique [0002] Cordyceps guangdongensis T.H.Li,Q.Y.Lin&B.Song, now named Tolypocladium guangdongense(T.H.Li,Q.Y.Lin&B.Song)V.Papp, was discovered and successfully domesticated by researcher Li Taihui from Guangdong Institute of Microbiology. The Ministry of Health of the People's Republic of China (now the National Health Commission of the People's Republic of China) officially approved as "new resource food" (No. 1, Ministry of Health of the People's Republic of China in 2013). Cantonese Cordyceps and Cordyceps sinensis belong to the same family of Cordyceps fungi, and their active ingredients and contents are very similar. Studies have shown that Cordyceps guangdong has significant effects in preventing and treating chronic renal failure, and also has the effects of treating chroni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02C12N13/00A01G18/00C12R1/645
CPCC12N1/02C12N13/00A01G18/00
Inventor 李泰辉张成花黄浩邓旺秋李挺
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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