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Culture method for cordyceps guangdongensis T.H.Li,Q.Y.Lin&B.Song

A cultivation method and technology of Cordyceps in Guangdong, applied in the directions of botanical equipment and methods, cultivation, plant cultivation, etc., can solve the problems of less loading, small cultivation area, low degree of automation, etc., and achieve the effect of improving yield and easy operation.

Pending Publication Date: 2021-02-05
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bottle culture has disadvantages such as less material loading, small culture area, low degree of automation, and low harvesting efficiency.

Method used

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  • Culture method for cordyceps guangdongensis T.H.Li,Q.Y.Lin&B.Song
  • Culture method for cordyceps guangdongensis T.H.Li,Q.Y.Lin&B.Song
  • Culture method for cordyceps guangdongensis T.H.Li,Q.Y.Lin&B.Song

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Liquid culture (preparation of seed liquid):

[0029] The slant species or shake-flask species were expanded in sequence and then transferred to the fermenter. The slant species were cultivated at 24±1°C for 10-15 days, and the surface spores were scraped and transferred to shake-flask culture. The shake-flask culture conditions were 150-160rpm, 24± Cultivate at 1°C for 5 to 6 days; transfer 5% of the shake flask species into a fermenter with a capacity of 200 liters, and the liquid volume of the fermenter is 120 liters for cultivation. It was 1.5vvm, and cultivated for 3 days; the dilution of the seed liquid: in another capacity, 600 liters of drinking water were filled in a fermenter of one ton, after sterilization and cooling, the seed liquid (total 120 liters) in the 200 liter fermenter was Transfer to a one-ton fermenter, mix evenly with sterilized water, and set aside.

[0030] Slant medium formula: Each 1L medium contains 200g of potatoes, 50g of soybean sprouts...

Embodiment 2

[0041] It is basically the same as in Example 1, the only difference is that in step a of the solid culture process and the preparation of the solid medium, drinking water (ie 0.6 liters) is added according to the ratio of material to water 1:1.

Embodiment 3

[0043] It is basically the same as in Example 1, the only difference is that in step a of the solid culture process and the preparation of the solid medium, 0.72 liter of nutrient solution is added according to the ratio of material to water 1:1.2.

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PUM

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Abstract

The invention discloses a culture method for cordyceps guangdongensis T.H.Li,Q.Y.Lin&B.Song. The method comprises the processes of liquid culture, solid culture and harvest, wherein the solid culture process comprises the following steps of a, preparing a solid culture medium, specifically, preparing the solid culture medium according to a material-water ratio of 1:(1-1.2); b, carrying out inoculation, specifically, inoculating the solid culture medium with a seed solution obtained via liquid culture; and c, carrying out solid culture.

Description

technical field [0001] The invention relates to a method for cultivating Cordyceps guangdong, belonging to the field of artificial cultivation of Cordyceps. Background technique [0002] Cordyceps guangdongensis T.H.Li,Q.Y.Lin&B.Song= [0003] Tolypocladium guangdongense (T.H.Li,Q.Y.Lin&B.Song)V.Papp) is a rare Cordyceps resource found only in Guangdong. After nearly ten years of research and development, it was approved as a new food raw material by the state in 2013. Studies have shown that Cordyceps guangdong has various medicinal effects such as anti-avian flu virus, anti-fatigue, life extension, treatment of chronic renal failure, treatment of chronic bronchitis, regulation of immunity and anti-oxidation, etc. It is a Cordyceps resource with great industrial value. [0004] Although Cantonese Cordyceps was approved as a new food raw material in 2013, the industrialization process of Cantonese Cordyceps has been restricted due to its slow growth. Publication number is ...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 谭悠久崔俏俏孙长胜唐瑶朱冰舟江可
Owner ZHEJIANG BIOASIA PHARMA CO LTD
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