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Cordyceps guangdongensis polysaccharides CCG-PSW and CCG-PSS, preparation method and application

A technology of CCG-PSW and polysaccharides, which is applied in the field of cosmetics, medicine, and health food. It can solve the problems of weakened cell membrane elasticity, inelasticity, and accelerated aging of the body, and achieve the effects of clear polysaccharide structure, controllable quality, and simple operation.

Active Publication Date: 2018-12-18
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as the organism ages, the self-regulation function of the body gradually decreases, resulting in some factors produced by metabolic activities in the body, hydrogen peroxide, superoxide free radicals, hydroxyl free radicals and other free radicals cannot be removed in time, and these accumulated in the body Free radicals will attack the cell membrane in the body, snatch its electrons, weaken the elasticity of the cell membrane or even lose its function, and cause diseases in the body. Free radicals may also attack genes, destroy their molecular structure, cause mutations in genes, and cause The life system disorder of the body accelerates the aging of the body (Frankel et al., 2000)

Method used

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  • Cordyceps guangdongensis polysaccharides CCG-PSW and CCG-PSS, preparation method and application
  • Cordyceps guangdongensis polysaccharides CCG-PSW and CCG-PSS, preparation method and application
  • Cordyceps guangdongensis polysaccharides CCG-PSW and CCG-PSS, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of Guangdong Cordyceps polysaccharide CCG-PSW and CCG-PSS

[0036] 1) The powder of Guangdong Cordyceps fruit body dried at 50℃ to a constant weight is crushed with a universal high-speed mixer and passed through a 60-mesh sieve to obtain

[0037] 2) Add petroleum ether to the fruit body powder of step 1) according to the material-to-liquid ratio of 1:5, degrease at room temperature until the solution is colorless, wait for the remaining petroleum ether in the residue to volatilize and dry;

[0038] 3) Weigh the residue obtained in step 2), add distilled water according to the material-to-liquid ratio of 1:20, and extract twice in a constant temperature bath at 90°C. The second extraction is 5000rpm after the first extraction After centrifugation for 20 minutes to collect the remaining residues of the supernatant, extract for 2 hours each time, stirring every 30 minutes in the middle, and combine the supernatants after the second extraction to obtain the ...

Embodiment 2

[0042] Example 2 Structural analysis of Guangdong Cordyceps polysaccharide CCG-PSW and CCG-PSS

[0043] Further structural analysis of CCG-PSW and CCG-PSS obtained in Example 1 is as follows:

[0044] (1) GC-MS analysis of monosaccharide composition

[0045] Using GC / MS method, use 2M H in a sealed glass tube 2 SO 4 (5mL) 10mg of CCG-PSW and CCG-PSS were hydrolyzed at 100°C for 8h. With BaCO 3 After the neutralization reaction, the suspension was collected, concentrated to a dry residue with a rotary evaporator, and then reacted with 10 mg of hydroxylamine hydrochloride and 0.5 mL of pyridine at 90°C for 30 minutes, and then 0.5 mL of acetic anhydride at 90°C was added to the glass tube React for 30 minutes, and finally detect in the GC instrument, flame atomic detector (FID), the test condition is: N 2 (50mL / min), H 2 (38mL / min), air (370mL / min), the initial column temperature is 130℃, after maintaining for 5min, it will increase to 240℃, increasing by 4℃ per minute (1min / ℃), the s...

Embodiment 3

[0056] Example 3 Determination of Antioxidant Activity of Guangdong Cordyceps Polysaccharide CCG-PSW and CCG-PSS

[0057] (1) The scavenging ability of CCG-PSW and CCG-PSS on hydroxyl radicals

[0058] The test sample reaction solution includes 1mL of 0.15M PBS (pH 7.4), 1mL of 40μg / mL safranin solution, 1mL of 0.945mM EDTA-Fe(Ⅱ), 1mL of 3%(V / V)H 2 O 2 , 0.5mL of polysaccharide sample solution (CCG-PSW or CCG-PSS).

[0059] The reference substance reaction solution includes 1mL of 0.15M PBS (pH 7.4), 1mL of 40μg / mL safranin solution, 1mL of 0.945mM EDTA-Fe(Ⅱ), 1mL of 3%(V / V)H 2 O 2 , 0.5mL of Vc solution.

[0060] The reaction solution of the test sample and the reaction solution of the reference substance were reacted at 37°C for 30 minutes. After the reaction, the absorbance A value was measured at 560nm. The blank control group used double distilled water instead of the polysaccharide sample solution. The calculation formula is as follows:

[0061] Scavenging rate of hydroxyl radical...

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Abstract

The invention discloses cordyceps guangdongensis polysaccharides CCG-PSW and CCG-PSS, a preparation method and application. The invention for the first time identifies the structures of cordyceps guangdongensis polysaccharides CCG-PSW and CCG-PSS, makes the physicochemical properties and structures of the two polysaccharide components clear, and further explores the antioxidant activity. The result indicates that: in antioxidant activity experiments, the CCG-PSW and CCG-PSS polysaccharide pure products have the effect of scavenging hydroxyl radicals, DPPH radicals and ABTS radicals, ferrous ion chelating ability and reducing ability, and the action force is concentration-dependent. The cordyceps guangdongensis polysaccharides CCG-PSW and CCG-PSS can be used for preparation of antioxidant cosmetics, food, health products or drugs.

Description

Technical field [0001] The invention relates to the technical fields of health foods, cosmetics and medicines, in particular to Guangdong cordyceps polysaccharide CCG-PSW and CCG-PSS, and preparation methods and applications. Background technique [0002] Guangdong Cordyceps (Cordyceps guangdongensis THLi, QYLin&B.Song), its fruiting body has been approved by the Ministry of Health as a "new food raw material" (the Ministry of Health 2013 Announcement No. 1), is the second new Cordyceps food after Cordyceps militaris Resources, studies have shown that the fruiting bodies of Cordyceps guangdong have antioxidants, life extension, treatment of renal failure and bronchitis, etc. (Zeng Hongbin, 2009; Yan Wenjuan et al., 2010, 2014; Yan et al., 2013, 2014). Therefore, Guangdong Cordyceps fruiting bodies can not only become a delicacy on people's table, but also have medicinal value, which has great development and application prospects. [0003] Cordyceps polysaccharide is a complex org...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/0087
Inventor 李泰辉陈香女邓旺秋李挺张成花宋斌
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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