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Cordyceps guangdongensis protoplast preparation and recovery method

A protoplast, Guangdong Cordyceps technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., to achieve the effect of promoting research and application progress and speeding up research speed

Active Publication Date: 2019-06-28
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different fungi have their own enzymatic hydrolysis systems and hydrolysis conditions. At present, there have been studies on the preparation and recovery of protoplasts in the Cordyceps fungi Cordyceps sinensis, Cordyceps militaris, Paecilomyces cicadae, and Cordyceps kaohsiung. However, the protoplasts of Cordyceps guangdong The preparation method has not been established yet, and the method of preparing Cordyceps protoplasts using the mixed enzyme system of lyase and collapse enzyme has not been reported yet

Method used

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  • Cordyceps guangdongensis protoplast preparation and recovery method
  • Cordyceps guangdongensis protoplast preparation and recovery method
  • Cordyceps guangdongensis protoplast preparation and recovery method

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Embodiment 1

[0025] The preparation of embodiment 1 Guangdong Cordyceps grass protoplast

[0026] (1) Preparation of culture medium and KC buffer:

[0027] YMPT liquid medium: Take 6g of malt extract, 10g of tryptone, 20g of glucose, and 6g of yeast extract, mix them, add deionized water to make up to 1L, sterilize at 121°C for 20min.

[0028] TB 3 Regeneration medium: Take 3g of yeast extract, 3g of acid hydrolyzed casein, 200g of sucrose, 15g of low-melting point agarose, add distilled water to make up to 1L, and sterilize at 121°C for 20min.

[0029] KC buffer: take KCl 13.42g, CaCl 2 1.665g, add deionized water to make up to 300mL, and sterilize at 121°C for 20min.

[0030] (2) Preparation and recovery of Guangdong Cordyceps protoplasts:

[0031] Inoculate the mycelium of Cordyceps guangdong into 200mL YMPT liquid culture medium, 120rpm, 24 ℃ cultivate 12d, obtain the tender mycelium (mycelia) of Cordyceps guangdong( figure 1 ). To prepare an enzyme solution, add 0.15 g of a mix...

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Abstract

The invention discloses a cordyceps guangdongensis protoplast preparation and recovery method. The preparation method comprises the steps of mycelium culture, protoplast preparation, protoplast purification and the like, wherein a mixed enzyme system containing lyase and driselase is adopted to enzymolyse a mycelium to obtain a protoplast of cordyceps guangdongensis, and a TB3 recovery culture medium is used for recovering the cordyceps guangdongensis protoplast. The cordyceps guangdongensis protoplast preparation and recovery method has the advantages that a cordyceps guangdongensis protoplast preparation and recovery system is firstly established, thereby being conductive to the later genetic transformation research of the cordyceps guangdongensis or the implementation of protoplast fusion and other technologies, increasing the research speed in the genetics, breeding and other aspects, being conductive to promoting the progress of the research and application of the system in the field of healthcare food and medicine, and bringing benefits to the society.

Description

technical field [0001] The invention belongs to the biological field of fungi, and in particular relates to a method for preparing and recovering Guangdong Cordyceps protoplasts. Background technique [0002] Cordyceps guangdongensis is a new species published in 2008, which has been successfully domesticated and established a mature fruiting body cultivation system. Its fruiting body is safe and non-toxic, and was officially approved by the Ministry of Health in 2013 as "new resource food" (now renamed as "new food raw material"). Experiments have found that the fruiting body of Cordyceps guangdong has significant pharmacological effects, including anti-avian influenza virus, anti-fatigue and life extension, treatment of chronic renal failure, treatment of chronic bronchitis, and immune regulation. Previous studies also found that Cordyceps guangdong contains rich metabolites, including polysaccharides, fatty acids, cordycepic acid, amino acids, adenosine, trace elements, ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 邓旺秋黄虹李泰辉张成花李挺
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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