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High-yield and high-quality Tolypocladium guangdongense cultivation strain as well as identification primer and identification method thereof

A technology of Cordyceps sinensis and strains in Guangdong, applied in the field of high-yield and high-quality Guangdong Cordyceps sinensis cultivation strains and identification primers, can solve the problems of harming consumers' rights and interests, misleading consumers, etc., and achieves the effects of good fruiting body traits, protection of rights and interests, and short growth cycle.

Active Publication Date: 2021-01-15
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This market behavior of pretending to be Cordyceps guangdong has seriously misled consumers and damaged the rights and interests of consumers. Therefore, it is extremely necessary to rectify the name of Cordyceps guangdong products, and it is also necessary to explain the traits and molecular characteristics of high-quality production strains of Cordyceps guangdong

Method used

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  • High-yield and high-quality Tolypocladium guangdongense cultivation strain as well as identification primer and identification method thereof
  • High-yield and high-quality Tolypocladium guangdongense cultivation strain as well as identification primer and identification method thereof
  • High-yield and high-quality Tolypocladium guangdongense cultivation strain as well as identification primer and identification method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Mycelium growth and characteristics of Guangdong Cordyceps strain GDGM 30035

[0024] Recipe for SPDA (Sprout Potato Dextrose Agar Medium): Cut peeled 200g potatoes into a volume of 1cm 3 Mix small pieces of soybean sprouts with 100g of fresh soybean sprouts, add 800mL of distilled water to cook for 12-15min, filter through 8 layers of gauze, add 20g of glucose and 15g of agar to the filtrate, add water to 1000mL, sterilize at 115°C for 20min, and set aside.

[0025] Take the mycelial block of Cordyceps guangdong strain GDGM 30035 with a diameter of about 8mm and inoculate it to the central position of the medium on the SPDA plate. And the mycelial blocks inoculated with Cordyceps guangdong strains GDGM 33006 and GDGM 43414 were used as controls. During the growth stage of mycelia, the culture should be protected from light. The temperature of the cultivation room should be controlled at 24°C, the relative humidity of the air should be controlled at 65%, and...

Embodiment 2

[0026]Example 2: Fruiting body growth and characteristics of Cordyceps guangdong strain GDGM 30035

[0027] After the mycelium is overgrown with the culture medium, increase the scattered light irradiation and use LED light strips for supplementary light. The light quality can be white light or blue light, the light intensity is 220lux, the light time is 12 hours a day, and the relative air humidity is kept at 75%. After 3-4 days, the hyphae turned from white to dark green, and after 9 days, the primordia appeared. Ventilate for the first time when the primordium emerges, that is, puncture the sealing film on the cultivation container to ventilate, and the CO in the cultivation room 2 The concentration is controlled at 0.4% v / v, CO in the container 2 The concentration was controlled at 4% v / v. The temperature is controlled at 23°C and the relative air humidity is 85%. Regularly turn on the internal circulation ventilation and external circulation fan every day to keep the a...

Embodiment 3

[0029] Example 3: Molecular biological characteristics of Cordyceps guangdong strain GDGM 30035

[0030] Genomic DNA extraction of fruiting bodies of Cordyceps guangdong strain GDGM 30035: Weigh 0.15 g of fruiting bodies of Cordyceps guangdong strain GDGM30035 and place them in a sterile EP tube, pour liquid nitrogen into them and grind them quickly. HiPure Fungal DNA Mini Kit from Magen Company ( Guangzhou Meiji Biotechnology Co., Ltd. (No. D3171-03) extracted genomic DNA from Cordyceps guangdong strain GDGM 30035.

[0031] Using ISSR nucleic acid molecule primer UBC822, the nucleotide sequence is (TC) 8 A, namely: 5'-TCTCTCTCTCTCTCTCCA-3' (synthesized by Shanghai Sangon Bioengineering Co., Ltd.), PCR amplification was carried out using the genomic DNA of the Cantonese Cordyceps strain GDGM 30035 as a template. The details are as follows: PCR reaction was carried out by using Premix TaqTM (TaKaRa TaqTM Version 2.0) kit (Dalian Baosheng Biological Takara, No.: R004A). The re...

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Abstract

The invention discloses a high-yield and high-quality Tolypocladium guangdongense cultivation strain as well as an identification primer and an identification method thereof. The high-yield and high-quality Tolypocladium guangdongense is a bacterial strain GDGM 30035, and the preservation number is GDMCC No: 61208. The strain has the characteristics of short growth period, good sporocarp characterand the like, and is suitable for large-area popularization. The invention also provides an identification primer and an identification method for identifying the Tolypocladium guangdongense cultivation strain GDGM 30035, and the Tolypocladium guangdongense cultivation strain GDGM 30035 can be accurately identified by adopting the identification primer and the identification method, so that the Tolypocladium guangdongense cultivation strain GDGM 30035 is prevented from being mixed with other types of cordyceps sinensis or other cordycepssinensis (mainly are cordyceps militaris) produced fromGuangdong; and misleading of consumers due to market behaviors of pretending Tolypocladium guangdongense by utilizing other types of cordyceps sinensis or other cordyceps sinensis (mainly are cordyceps militaris) produced from Guangdong is avoided, so that rights and interests of the consumers are guaranteed.

Description

technical field [0001] The invention belongs to the technical field of edible fungi, and relates to a high-yield and high-quality cultivated strain of Cordyceps guangdong and its identification primer and identification method. Background technique [0002] Cordyceps guangdongensis T.H.Li, Q.Y.Lin&B.Song, now named Tolypocladium guangdongense(T.H.Li, Q.Y.Lin&B.Song) V.Papp, was discovered by researcher Li Taihui from Guangdong Institute of Microbiology and successfully domesticated and cultivated. The fruiting body of Cordyceps guangdong is dark green to gray green, thin columnar or rod-shaped. It was preserved in China Center for Type Culture Collection (CCTCC) in 2006, and the preservation number is: CCTCC No.M206051. In 2010, it was proved by the toxicology test of Guangdong Provincial Center for Disease Control and Prevention that the fruiting body of Cordyceps guangdong was safe and non-toxic, and it was released on January 2013. It was officially approved as "new reso...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N15/11C12Q1/6895C12Q1/686C12Q1/04C12R1/645
CPCC12Q1/6895C12Q1/686C12R2001/645C12N1/145C12Q2565/125
Inventor 李挺李泰辉张成花邓旺秋黄浩宋斌
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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