The invention discloses a nutritional biscuit eaten after running and a preparation method thereof. The nutritious biscuit is prepared from raw materials including 300-1,000 parts of wheat flour, 100-500 parts of white granulated sugar, 15-35 parts of corn starch, 20-100 parts of black rice flour, 10-80 parts of egg white, 50-200 parts of oil, 0.1-5 parts of table salt, 2-5 parts of ammonium bicarbonate, 1-5 parts of sodium bicarbonate, 0.005-0.05 part of citric acid, 2-10 parts of a burdock root extract, 2-10 parts of a radix fici simplicissimae extract, 0.2-3 parts of calcium carbonate, 2-10 parts of pollen pini, 0.2-10 parts of moringa leaf powder, 5-80 parts of dried fruits and 0.02-2.5 parts of edible essence through processes of ingredient preparation, dough production, forming, baking and the like. The nutritional biscuit eaten after running adopts a medicinal and edible food formula, has comprehensive nutrition, can quickly supplement energy, relieve fatigue after exercise, nourish qi and blood, strengthen the spleen and stomach, relax tendons, activate collaterals, dispel cold and promote eruption, is convenient to eat, tastes good and is suitable for people requiring quick energy supplement as well as people requiring energy supplement after exercise, particularly after running.