30 results about "Beta-farnesene" patented technology

The invention constructs a beta-farnesene producing bacterium through genetic engineering, and the beta-farnesene producing bacterium is preserved in the China General Microbiological Culture Collection Center, CGMCC, wherein the preservation number is CGMCC No.16709. The beta-farnesene producing bacterium can realize effective accumulation of beta-farnesene, the yield of the beta-farnesene is ashigh as 250mg/L, and the beta-farnesene producing bacterium has industrial prospects.

The invention discloses a (trans)-beta-farnesene analogue containing a jasmonic acid base group, as well as preparation and application of the (trans)-beta-farnesene analogue belonging to the field of organic chemistry synthesis. The preparation of the (trans)-beta-farnesene analogue comprises the following steps of adding alkali and a condensing agent to an organic solvent, and in the organic solvent, enabling compounds as shown in a formula III or a formula V to be subjected to a condensation reaction so as to obtain the (trans)-beta-farnesene analogue containing the jasmonic acid base group, as shown in a formula I or a formula II. A synthetic method is simple to operate, raw materials are easy to obtain, harmful by-products are not produced, and the obtained products are stabler than trans-beta-farnesene. The (trans)-beta-farnesene analogue containing the jasmonic acid base group shows multiple bioactivity; the (trans)-beta-farnesene analogue with high dosage has good killing activity on budworms, and the (trans)-beta-farnesene analogue with low dosage has expelling activity on the budworms, so that the (trans)-beta-farnesene analogue has a high application development value.

The invention provides a thrip attractant and insecticide combined trapping and killing method. An attractant is prepared by taking liquid paraffin as a solvent and mixing 100 [mu] g/ml of methyl nicotinate, trans-beta farnesene, caprylaldehyde, 3-carene, isoeugenol and beta-phenethyl alcohol with the same mass. The attractant disclosed by the invention contains specific host plant source volatilesubstances, is diverse in variety, wide in action target sites and strong in complementarity; a compound with a relatively strong trapping effect on thrips is prepared according to a certain proportion; thrips are trapped on an insect sticking color plate, and meanwhile, thrip injurious insecticide high-efficiency and low-residue pesticide is sprayed on the insect sticking color plate; the thripsare killed by utilizing the toxicity of the insecticide, so that the thrips are thoroughly killed, and secondary escape of thethrips is prevented from causing harm. The attractant disclosed by the invention has the characteristics of low cost, environmental protection, high efficiency, low possibility of generating drug resistance and the like; and the trapping substance is derived from plants, is safe and reliable, can be widely applied to vegetables, fruit trees and other crops, and has wide application prospects.

The invention discloses an attractant for Anoplophora glabripennis adults. The attractant comprises (E)-beta-Farnesene or Farnesene containing six isomers of the (E)-beta-Farnesene, (Z)-beta-farnesene, (E,E)-alpha-Farnesene, (Z,Z)-alpha-Farnesene, (E,Z)-alpha-Farnesene and (Z,E)-alpha-farnesene; the attractant can be used for attracting female and male Anoplophora glabripennis adults; besides, the attractant can be used for attracting female and male adults in field, and provides a practical nuisanceless monitoring and control technical means for monitoring the occurrence period and the occurrence amount of the adults or large-scale trap and kill in forest.

The invention discloses an efficient preparation method and application of poly beta farnesene with ultra-high molecular weight. The invention belongs to the field of polymer synthesis. The inventionaims to solve the technical problems of low molecular weight of poly beta farnesene obtained by existing methods and higher cost of adopted rare earth metal reactants. The method comprises the following steps: adding a solvent, a main catalyst, a cocatalyst and a beta-farnesene monomer into a reactor according to any sequence under the protection of inert gas, performing polymerizing for 10-240 minutes at 0-100 DEG C while stirring, adding a quenching agent and an anti-aging agent into the reaction system for quenching reaction, repeatedly performing washing with ethanol, and carrying out vacuum drying to obtain the poly beta-farnesene . The main catalyst is a bipyridine iron complex. The microstructure of the obtained poly beta-farnesene is composed of 3,4-poly beta-farnesene with the molar fraction of 50%-70% and 1,4-poly beta-farnesene with the molar fraction of 30%-50%, the number-average molecular weight of the obtained poly beta-farnesene ranges from 8.0 x 10<5> g/mol to 1.7 x 10<6> g/mol, and the molecular weight distribution ranges from 1.5 to 3.5.

The invention relates to a (cis)-beta-farnesene analog containing a 1,2,3-thiadiazole group, a preparation method and applications thereof, and belongs to the field of organic synthesis. The 1,2,3-thiadiazole group containing (cis)-beta-farnesene analogs with different substituents are obtained through the condensation reactions among geraniol, geranylamine/substituted geranylamine, and 4-R1-1,2,3-thiadiazole-5-formic acid; and then the analogs are separated and purified by silica gel column chromatography to obtain a compound represented by the formula I. The provided synthesis method has the advantages of simple operation, easily-available raw materials, and difficulty in generating harmful byproducts, and moreover, the target compound is more stable than cis-(beta)-farnesene. The provided analog has multiple bioactivities, analog with a high dosage can kill aphid, analog with a low dosage can repel aphid, at the same time the analog can prevent tobacco mosaic virus and kill bacteria, and thus the analog is worth to develop.

The invention discloses a plant aphid resistance realization method based on a 35S promoter. The method comprises the following steps: cloning to obtain EbetaF synthetase genes EbetaFS from peppermintMenthaXpiperita, constructing an expression vector, transferring the vector into agrobacterium, infecting wheat embryos with the agrobacterium, catalyzing FPP to synthesize trans-beta-farnesene in wheat, synthesizing aphid alarm toxin trans-beta-farnesene in the wheat, and transporting to transgenic wheat to release the trans-beta-farnesene, thereby achieving the effect of dispelling aphids.

The invention relates to a method for obtaining young winged aphids of macrosiphum avenae in a rapid and directional mode. The method comprises the steps that S1, adult wingless aphids of macrosiphum avenae are raised in a single mode, and propagation of three generation is carried out; S2, aphids of the fourth generation are raised to third instar in a single mode, and a plurality of raised aphids of the fourth generation are put into a same culture device; S3, E-beta-farnesene solutions are added into the culture device repeatedly, and after the E-beta-farnesene solutions are added, young winged pseudo embryos and/or aphids of macrosiphum avenae are collected. According to the method for obtaining the young winged aphids of macrosiphum avenae in a directional mode, pseudo embryos which can develop winged macrosiphum avenae, first instar aphids and second instar aphids which can develop winged macrosiphum avenae, third instar aphids of winged macrosiphum avenae, fourth instar aphids of winged macrosiphum avenae and adult aphids of winged macrosiphum avenae are obtained in a directional mode, and the basis of the aphid gene expression study of the pseudo embryo period, first instar period and second instar period is provided; the method is convenient, simple and easy to operate, and wide in application prospect.

The invention relates to a ubiquitin-1 promoter-based plant aphid resistance implementation method. An E-beta-farnesene synthase (EbetaFS) gene is cloned from peppermint by means of genetic engineering, and a ubiquitin-1 promoter is constructed for driving an EbetaF vector. An gene is transferred into wheat by using a gene gun transformation method; E-beta-farnesene is synthesized by catalyzing FPP in the wheat; the release of the E-beta-farnesene in transgenic wheat is realized by synthesizing an aphid alarm element E-beta-farnesene in the wheat, so that the aim of avoiding aphids is achieved.

The invention relates to the technical field of biological fermentation, in particular to a method for extracting beta-farnesene from fermentation broth. The method comprises the step S1 of dual-phaseextraction fermentation, the step S2 of flocculation removal, the step S3 of water vapor vacuum distillation and the step S4 of oil-water separation. According to the method, the loss of a target product in the fermentation process is greatly reduced by adding an organic solvent in the fermentation stage for duplex fermentation. A flocculant is added to remove residual bacteria, proteins and small molecular impurities existing in the downstream product extraction process, and the solvent extraction process and the water vapor distillation process are coupled to effectively increase the yieldand the purity of the beta-farnesene. The method breaks through the bottleneck of separation and purification technology of high-purity beta-farnesene products, achieves continuous production, significantly reduces the energy consumption of production, especially improves the recycling rate of the organic solvent, reduces the production of organic wastewater and achieves clean production.

The invention relates to a method for preparing beta-farnesene by fermentation of sugar sorghum stalks and sugarcane waste residues. The method comprises the following steps of: A, harvesting and squeezing mature and fresh sugar sorghum to obtain sugar sorghum juice; B, concentrating the sugar sorghum juice; C, uniformly mixing a sugar sorghum juice concentrated solution with the sugarcane waste residues which are by-products produced in a sugar refinery, and obtaining a mixed solution of the sugar sorghum and the sugarcane waste residues; D, taking the mixed solution of the sugar sorghum and the sugarcane waste residues as a carbon source of fermentation, and taking Saccharomyces cerevisiae of which the collection number is CICC No. 32475 as a strain to prepare a shake flask strain; E, inoculating the shake flask strain to a seed culture medium to be cultured; G, inoculating a seed culture fluid to a fermentation medium to be fermented for 30 to 50 hours, adding a flocculating agent to a fermentation broth, and obtaining a supernatant through siphoning after precipitation. According to the method for preparing beta-farnesene by the fermentation of the sugar sorghum stalks and the sugarcane waste residues disclosed by the invention, the adopted raw materials are low in cost, and the content of the beta-farnesene in the obtained supernatant is high, thereby being beneficial to reduction of the preparation cost of squalane derived from plants.

The invention relates to the technical field of biological mutagenesis breeding, and in particular, relates to a compound mutagenesis breeding method of a mutant strain for highly yielding beta-farnesene. The method includes the steps: preparation of a bacterial suspension, plasma-ultraviolet compound mutagenesis, flat plate primary screening, shake flask re-screening and fermentation tank verification. According to the compound mutation breeding method of the mutant strain for highly yielding beta-farnesene, through plasma-ultraviolet compound mutagenesis, flat plate primary screening and shake flask re-screening are adopted, and finally, the fermentation unit of beta-farnesene of the mutant strain for highly yielding beta-farnesene in the fermentation tank is 169.10% higher than that ofa starting strain, and the mutant strain still has good hereditary stability through 8 times of consecutive passage.