Method for extracting beta-farnesene from fermentation broth

A fermentation broth and farnesene technology, applied in the field of extracting β-farnesene, can solve the problems of unsuitability for industrial production, low yield, time-consuming, etc., achieve a breakthrough in the separation and purification technology bottleneck, high product yield, and reduce production cost effect

Inactive Publication Date: 2019-06-14
山东昆达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, steam distillation is one of the most commonly used extraction methods for β-farnesene, which has the characteristics of simple equipment, easy operation and low cost, but this method is time-consuming and low in yield, and is not suitable for industrial production
At present, there is no report on the method of separating and extracting high-purity β-farnesene from microbial fermentation broth with complex components

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 culture medium

[0026] A seed medium was prepared, and the components of the seed medium were: 10 g / L tryptone, 5 g / L yeast extract and 10 g / L NaCl.

[0027] Prepare the fermentation medium, the components of the fermentation medium are: 2.3g / L citric acid, 0.3g / L ferric amine citrate, 5g / L yeast extract, 20g / L glycerol, 2g / L glucose, 3.07g / L L MgSO 4 .7H 2 O, 7.24g / L KH 2 PO 4 .

Embodiment 2

[0028] Example 2 A method for extracting β-farnesene from fermentation broth

[0029] A method for extracting β-farnesene from fermentation broth, comprising the steps of:

[0030] S1, two-phase extraction fermentation

[0031] (1) Cultivation of primary seed bacteria: add the seed culture medium prepared in Example 1 to the seed tank, insert Escherichia coli K-12MG1655 seed solution according to the inoculum amount of 1% after sterilization, and control the pH to 6.5, Rotate and shake for 12 hours, and transplant to the breeding tank when the biomass is 2-4%;

[0032] (2) Cultivation of secondary seed bacteria: add 30L of the seed medium prepared in Example 1 to the breeding tank, insert the primary seed liquid of E. coli according to the inoculation amount of 1% after sterilization, control the pH to 6.5, and rotate Shake culture for 18 hours, and transplant to the fermenter when the biomass reaches 5-8%;

[0033] (3) Cultivation of strains in the fermenter: add 100L of t...

Embodiment 3

[0041] Example 3 A method for extracting β-farnesene from fermentation broth

[0042] A method for extracting β-farnesene from fermentation broth, comprising the steps of:

[0043] S1, two-phase extraction fermentation

[0044] (1) Cultivation of primary seed bacteria: add the seed culture medium prepared in Example 1 to the seed tank, insert Escherichia coli K-12MG1655 seed solution according to the inoculum amount of 1% after sterilization, and control the pH to 6.5, Rotate and shake for 12 hours, and transplant to the breeding tank when the biomass is 2-4%;

[0045] (2) Cultivation of secondary seed bacteria: add 30L of the seed medium prepared in Example 1 to the breeding tank, insert the primary seed liquid of E. coli according to the inoculation amount of 1% after sterilization, control the pH to 6.5, and rotate Shake culture for 18 hours, and transplant to the fermenter when the biomass reaches 5-8%;

[0046] (3) Cultivation of strains in the fermenter: add 100L of t...

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Abstract

The invention relates to the technical field of biological fermentation, in particular to a method for extracting beta-farnesene from fermentation broth. The method comprises the step S1 of dual-phaseextraction fermentation, the step S2 of flocculation removal, the step S3 of water vapor vacuum distillation and the step S4 of oil-water separation. According to the method, the loss of a target product in the fermentation process is greatly reduced by adding an organic solvent in the fermentation stage for duplex fermentation. A flocculant is added to remove residual bacteria, proteins and small molecular impurities existing in the downstream product extraction process, and the solvent extraction process and the water vapor distillation process are coupled to effectively increase the yieldand the purity of the beta-farnesene. The method breaks through the bottleneck of separation and purification technology of high-purity beta-farnesene products, achieves continuous production, significantly reduces the energy consumption of production, especially improves the recycling rate of the organic solvent, reduces the production of organic wastewater and achieves clean production.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a method for extracting β-farnesene from fermentation broth. Background technique [0002] β-Farnesene is a sesquiterpene volatile organic compound and an important chemical raw material widely used in agriculture, medicine, energy and other fields. At present, my country's β-farnesene production technology is under development and development. There are few manufacturers and backward production technology. The scale is small, the output is low, the cost is high, the raw materials are difficult to obtain and the quality is unstable, and it is difficult to meet the market demand. Competing with advanced countries such as Japan, Europe, and the United States will not allow products to enter the international market. Trace it to its cause, mainly be that we have not mastered a set of advanced separation and extraction technology yet. [0003] In the process of synth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P5/02C07C11/21C07C7/12C07C7/04C07C7/09C12R1/19
CPCY02E50/30
Inventor 寇凤雨马杰希刘伟刘瑞艳张彩霞康建兵
Owner 山东昆达生物科技有限公司
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