78 results about "Agalactosis" patented technology

The invention relates to immunogenic polysaccharide-protein conjugates comprising a capsular polysaccharide (CP) from Streptococcus agalactiae, commonly referred to as group B streptococcus (GBS), and a carrier protein, wherein the CP is selected from the group consisting of serotypes Ia, Ib, II, III, IV, V, VI, VII, VIII, and IX, and wherein the CP has a sialic acid level of greater than about 60%. The invention also relates to methods of making the conjugates and immunogenic compositions comprising the conjugates. The invention also relates to immunogenic compositions comprising polysaccharide-protein conjugates, wherein the conjugates comprise a CP from GBS serotype IV and at least one additional serotype. The invention further relates to methods for inducing an immune response in subjects against GBS and/or for reducing or preventing invasive GBS disease in subjects using the compositions disclosed herein. The resulting antibodies can be used to treat or prevent GBS infection via passive immunotherapy.

The invention aims at providing a traditional Chinese medicine for adjusting the insufficient lactation and the agalactosis of sow, wherein the traditional Chinese medicine is good in treatment effects, free from side effects, and selected from pure natural good-quality traditional Chinese medicine materials. The traditional Chinese medicine is characterized by comprising the following raw materials, i.e. phaseolus calcaratus, the seed of cowherb, tetrapanax papyriferus, sweetgum fruit, eucommia ulmoides, herba lycopi, leonurus, astragalus mongholicus, Chinese angelica, codonopsis pilosula, herba epimedii, semen cuscutae, the fruit of Chinese wolfberry, glossy privet fruit, dandelion, soybean, sesame and crucian. A preparation method of the traditional Chinese medicine comprises the following steps of: removing the entrails of the crucian, putting into a marmite with the raw materials, adding water of which the quantity is three times that of the mixture, boiling by big fire, stewing by soft fire for 30-40min, and removing slag of filter liquor; feeding the liquid medicine to the sow after the liquid medicine is cooled three times per day and 500g per time, and stopping feeding the liquid medicine after the traditional Chinese medicine becomes effective. The liquid medicine is fed to the sow after the method of the traditional Chinese medicine is adopted, so that the sow can quickly recover the bodily function, the fecundity and the premonition can be improved, the appetite can be enhanced, the digestion can be promoted, the traditional Chinese medicine has the function of promoting lactation, and the quality and the quantity of the milk of the sow can be improved, so that the sow health can be guaranteed, and the sow has strong disease resistance.

The invention discloses a preparation method and application of a tilapia source streptococcus agalactiae recombinant GroEL protein vaccine. The recombinant GroEL protein vaccine is prepared by tilapia source streptococcus agalactiae recombinant GroEL protein. An amino acid sequence of the tilapia source streptococcus agalactiae recombinant GroEL protein is as shown in SEQ ID NO.2. In the invention, tilapia source streptococcus agalactiae is taken as a research object, a GroEL gene is obtained from a tilapia source streptococcus agalactiae genome by cloning, the cloned GroEL gene is connected with a pET28a(+) carrier to construct an expression vector, and the vector is subjected to prokaryotic expression and purification in E.coli BL21(DE3) to obtain the recombinant GroEL protein vaccine with strong specificity and an immune protective efficacy of 68.61+/-7.39 percent, so that a foundation is laid for immune prevention and treatment of a tilapia streptococcus agalactiae disease.

The invention discloses an application of plectasin in inhibiting streptococcus agalactiae. The plectasin is found to be good at inhibiting the streptococcus agalactiae through bacteriostasis experiments; the plectasin is further prepared into corresponding agentia, such as plectasin gel and plectasin emulsion, the agentia achieve good curative effects for mastitis, wherein the antibacterial anti-inflammatory effect of the gel is better, the action time lasts longer, the moisturizing effect is better, action sites can be effectively isolated from making contact with air, and a wound is prevented from infection and inflammation. The plectasin can be used for preparing bacteriostat of the streptococcus agalactiae and further used for curing the mastitis, and especially for the mastitis caused by the streptococcus agalactiae which is difficult to cure at present, and a good curative effect is achieved.

The invention discloses a rapid separation and identification kit for streptococcus agalactiae. In the kit, the following reagents are included: sheep defibering blood plate, 3% H2O2, staphylococcus aureus strains, a specific PCR (polymerase chain reaction) solution, and positive control DNA (deoxyribonucleic acid). The rapid separation and identification kit for streptococcus agalactiae provided by the invention has the following beneficial effects that bacterial morphological observation, catalase test, CAMP (cyclic adenylic acid) reaction and PCR detection are used together, so that identification procedures can be simplified, the specificity is strong, and the sensitivity is increased by more than 15%; and moreover, the identification cost is low, the time consumption is short (only needing 3 days), and streptococcus agalactiae strains can be obtained by separation and accurately identified. The kit can be used for separation and identification for the samples of the milk samples of human or dairy cows, cervical mucus, vaginal swabs and the like.

The invention provides a LAMP (Loop-Mediated Isothermal Amplification) primer group, a micro-fluidic chip and a kit for detecting reproductive tract pathogenic microorganisms, and belongs to the technical field of reproductive tract infection detection. The LAMP primer group comprises a streptococcus agalactiae detection primer group, an enterococcus faecalis primer group, a gardnerella vaginosisprimer group, a Candida albicans primer group and a Chlamydia trachomatis primer group. The above LAMP primer group is fixed on the micro-fluidic chip. The LAMP primer group, the micro-fluidic chip and the kit can quickly, sensitively and accurately detect streptococcus agalactiae, enterococcus faecalis, gardnerella vaginosis, Candida albicans and Chlamydia trachomatis by high repeatability.

The invention relates to a culture-independent semiquantitative detection kit of tilapia streptococcus agalactiae, which consists of a reagent A, a reagent B, a reagent C, a reagent D, a reagent E, a product specification and a test result record card. The kit can be used for directly detecting whether blood of tilapia carries the streptococcus agalactiae without pure culture by a method provided by the invention, and semiquantitatively detect the quantity of the streptococcus agalactiae in the blood.

The invention relates to a loop-mediated isothermal amplification primer for detecting streptococcus agalactiae of tilapias. The primer is designed according to a conserved sequence of a surface immunogenic gene of the streptococcus agalactiae of the tilapias and comprises sip-F3, sip-B3, sip-FIP and sip-BIP respectively shown by SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. A loop-mediated isothermal amplification method for detecting the streptococcus agalactiae of the tilapias can be established according to the designed primer. In the method, cross reactions with several common pathogenic bacteria of aquatic animals and several common pathogenic bacteria of streptococcus cannot happen. The detection method provided by the invention does not need expensive instruments, and is short in time, simple in operation and high in sensitivity.

The invention discloses a preparation method and application of a micro-capsule coated egg yolk antibody IgY resistant to main pathogenic bacteria of dairy cow mastitis. A compound mycoprotein antigen is prepared by selecting streptococcus agalactiae, streptococcus dysgalactiae, staphylococcus aureus and escherichia coli and is mixed with an equal quantity of freund's adjuvant to prepare a multivalence antigen immune complex, laying hens are immune by adopting repeatedly alternative immune procedures and a muscular and subcutaneous multipoint injection means, a specific yolk immunoglobulin IgY resistant to the main pathogenic bacteria of dairy cow mastitis, namely DP-BM-IgY, is separated and purified from collected egg yolk, then homogeneous emulsification is conducted on the specific yolk immunoglobulin and a sodium alginate water solution and an emulsifying agent soya bean salad oil containing span-80, the emulsified solution is dropwise added into an encystation solution prepared from CaCl2 and chitosan for coating and curing, and the coated micro-capsules can effectively protect the activity of the DP-BM-IgY and have enteric solubility. The preparation method utilizes a micro-capsule technology and selects the chitosan and sodium alginate as coating materials (natural polysaccharides), and the egg yolk antibody is internally taken and free of any toxic or side effect.

The invention belongs to the technical field of aquaculture preparations, and discloses a feed additive for preventing streptococcus agalactiae, being prepared from the following components by mass percent: 10-15 percent of coptis chinensis, 7-15 percent of honeysuckle, 7-15 percent of isatis root, 10-20 percent of Houttuynia cordata, 20-40 percent of black soya bean and 0-20 percent of dendrobe. The additive is added into tilapia feed, has a highest immune protective rate of 95.21 percent for tilapia, and has practical and wide application values; the survival rate of the tilapia is 93.67 percent.

The invention discloses a health medicined diet for the treatment of postparturient agalactia, and the health medicined diet is prepared from the following main raw materials in parts by weight: 1.5-2.5 parts of seven-hole pig trotters, 1.0-2.0 parts of crucian, 0.4-0.8 part of chickweed, 1.0-1.5 parts of luffa, 0.8-1.2 parts of astragalus membranaceus, 0.3-0.5 part of juggins mandshurica fruit nuts, 0.1-0.2 part of eguanshi, 0.2-0.5 part of artemisia anomala, 0.8-1.5 parts of angelica sinensis, 0.3-0.5 part of pleione bulbocodioides, and 0.2-0.4 part of chastetree. The health medicined diet has the functions of conducing circulation and promoting circulation of human milk, eliminating stagnation to stop pain, clearing heat and dispersing blood stasis, and can be used for effectively enhancing the secretion of milk.

The invention provides a nursing agent for balancing a gynecological micro-ecological flora structure. The nursing agent comprises water, a surfactant, a humectant, a preservative and a thickening agent, and core functional components comprise a composition of micromolecular organic acid and a composition of saccharides and saccharide derivatives. The care agent realizes an obvious sterilization effect on escherichia coli, staphylococcus aureus and streptococcus agalactiae and an obvious bacteriostasis effect on candida albicans, effectively inhibits the growth of harmful bacteria, also can promote the growth of probiotic lactobacillus in the genital tract, and really plays a role in balancing healthy micro-ecological flora.

The invention discloses a drug for preventing and treating sow agalactosis and breast distending pain and a preparation method thereof. The drug is prepared from, by mass, 120-180 parts of akebiaquinata, 70-100 parts of safflower carthamus, 190-230 parts of dandelion, 110-180 parts of snow lotus herbs, 120-200 parts of leonurus, 130-210 parts of schizonepeta, 200-250 parts of herba lycopi, 120-190 parts of herba patriniae, 200-280 parts of ricepaper pith, 100-140 parts of ginseng, 200-250 parts of astragalus membranaceus and 250-320 parts of polygonum orientale. According to the drug, the postpartum anti-infection ability of sows can be enhanced, the sow lactation yield is increased, the lactating sow feed intake can be significantly increased, the reproductive performance of the sows is improved, and the piglet growth performance is improved.

The invention discloses a functional food for treating women's mastopathy, wherein the food is prepared from deer horn cap through steps of extracting, heating to 50-115 deg C and cooling down, husking and removing tendon, carrying out biological mildew, disintegrating and breaking cell wall, obtaining powdered antler with grain size below 180 meshes, disintegrating sika deer venison and mixing homogeneously with deer horn cap powder, proportioning with wild chrysanthemum flower, honeysuckle flower and pagodatree flower. The food can be used for treating women's lactiferous gland, mastadenitis, mammary cancer and agalactosis.