LAMP (Loop-Mediated Isothermal Amplification) primer group, micro-fluidic chip and kit for detecting reproductive tract pathogenic microorganisms

Abstract

The invention provides a LAMP (Loop-Mediated Isothermal Amplification) primer group, a micro-fluidic chip and a kit for detecting reproductive tract pathogenic microorganisms, and belongs to the technical field of reproductive tract infection detection. The LAMP primer group comprises a streptococcus agalactiae detection primer group, an enterococcus faecalis primer group, a gardnerella vaginosisprimer group, a Candida albicans primer group and a Chlamydia trachomatis primer group. The above LAMP primer group is fixed on the micro-fluidic chip. The LAMP primer group, the micro-fluidic chip and the kit can quickly, sensitively and accurately detect streptococcus agalactiae, enterococcus faecalis, gardnerella vaginosis, Candida albicans and Chlamydia trachomatis by high repeatability.

Description

technical field [0001] The invention belongs to the technical field of reproductive tract infection detection, and in particular relates to a LAMP primer set, a microfluidic chip and a kit for detecting reproductive tract pathogenic microorganisms. Background technique [0002] Reproductive tract infection (RTI) refers to a group of infectious diseases that occur in the reproductive system. Female RTI is a global social and public health problem. It is a general term for the infection of the female reproductive system by pathogenic microorganisms such as bacteria, viruses, Candida, Trichomonas, Chlamydia, and Mycoplasma. Wide range of features. [0003] Infection is the source of many diseases. If the reproductive tract infection is not timely diagnosed and treated correctly, it can increase the risk of AIDS and cervical cancer, and cause infertility, ectopic pregnancy, miscarriage, stillbirth, and death. Complications such as childbirth, premature birth, congenital infect...

AI Technical Summary

Problems solved by technology

The culture method itself has many defects and cannot meet the clinical needs

First of all, the culture method takes a long time to detect, and the culture time is related to the type of bacteria infected. It takes at least 2-3 days. When an acute disease occurs clinically, the culture method cannot be applied; secondly, the culture method has low accuracy, especially for batch identification. It is easy to infect each other; and it is difficult to detect bacteria that require special culture conditions; once again limited by the safety protection conditions of domestic laboratories, laboratory staff have a high risk of infection when isolating and cultivating pathogenic bacteria; the final culture The method has high requirements on the experimental conditions and requires many types of culture medium. The detection method of bacterial culture cannot be used in township hospitals, community medical units and outdoors, which is easy to cause delays to patients.

[0007] For the common PCR (Polymerase Chain Reaction, Polymerase Chain Reaction) method for identifying pathogenic microorganisms, because of its high requirements for the laboratory, special equipment is required, and it is easy to appear Disadvantages such as false positives and cross-contamination are also not conducive to the development of its application

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