75results about How to "Wide substrate adaptability" patented technology

The invention provides short-chain dehydrogenase obtained from Empedobacter brevis, a gene of the short-chain dehydrogenase, recombinant short-chain dehydrogenase, a recombinant expression vector with the gene, a genetically engineered bacterium, a preparation method of the recombinant short-chain dehydrogenase and application of the short-chain dehydrogenase or the genetically engineered bacterium containing the short-chain dehydrogenase to asymmetric reduction of prochiral ketones for preparation of optical pure chiral alcohols. The short-chain dehydrogenase for preparation of the chiral alcohols by asymmetric reduction has remarkable advantages and can be used for synthesis of high-optical-purity chiral alcohols (ee>99%). Moreover, easiness in preparation of catalysts, mild reaction conditions, high substrate adaptability and environment friendliness are realized, asymmetric reduction of the prochiral ketones can be efficiently catalyzed by recombinant cells in an isopropanol-containing reaction system without any coenzymes, and industrial application and development prospect is promising.

The invention provides a method for synthesizing a 2-fluoro-N-substituted aryl acetamide compound. The method comprises the following steps that a N-substituted aryl acetamide compound reacts at 20 to150 DEG C under the condition of existence of palladium catalysts, fluorinated reagents and additives; TLC tracking detection is performed until the reaction is thoroughly performed; through aftertreatment, the compound shown as a formula II is obtained; the amide substituent ortho-position high-selectivity aryl hydrocarbon bond direct fluorination can be mildly realized. The method has the advantages that the reaction conditions are mild; the operation is simple; the substrate applicability is high; the fluoridation selectivity is high, and the like. High application study values are realized.

The present invention provides a method for synthetizing a 2-fluoro phenol compound shown in a formula IV. The phenol compound shown in the formula I is prepared into a 2-pyridine oxygroup arene compound shown in a formula II through an Ullmann reaction, the 2-pyridine oxygroup arene compound shown in the formula II is mixed with a palladium catalyst, a fluorinating reagent, an additive and an organic solvent, the mixture is stirred under the temperature of 30-160 DEG C to perform a fluorination reaction to obtain an ortho-position fluoridated 2-pyridine oxygroup arene compound shown in a formula III, and the ortho-position fluoridated 2-pyridine oxygroup arene compound shown in the formula III is prepared into the 2-fluoro phenol compound shown in the formula IV through the action of alkali. The method provided by the present invention has the advantages of mild reaction conditions, simplicity in operations, good substrate adaptability, high fluorination selectivity and the like. The 2-fluoro phenol compound is shown in the figure below.

The invention discloses a synthesis method of a 2-aryl benzoxazole derivative, wherein the method comprises the steps: carrying out ultrasonic reaction on an o-nitrophenol derivative and an aryl formaldehyde derivative in the presence of a pyridine accelerator and an elemental sulfur reducing agent to obtain the 2-aryl benzoxazole derivative. The method has the advantages of one-pot reaction synthesis, simple steps, easily available accelerant, low cost, high yield and environmental friendliness, and is beneficial to industrial production and application.

The invention discloses a Rhodococcus (Rhodococcus sp.ECU0066) and the use thereof, and the culture collection number of the strain is CGMCC No.2547. The resting cells of the strain is taken as a biological catalyst, and prochiral phenyl alkyl sulfide and a derivative thereof are carried out catalytic oxidation asymmetrically to obtain benzoylate sulfoxide and a derivative thereof with optical activity. The strain and the stereoselective biological oxidation process have the advantages of better catalysis effect, simple and safe operation and low cost, the product is easily purified and is friendly to environment.

The invention discloses a synthesis method of GalNAc<alpha>1, 3Gal or Gal<alpha>1, 3Gal glycosidic bond oligosaccharide. The method comprises the following steps: a non-reductive tail end Gal of target oligosaccharide is subjected to <alpha>1, 2-fucosylation modification, so that a substrate can be recognized by <alpha>1, 3-N-acetylaminogalatose glycosyltransferase or <alpha>1, 3-galactose glycosyltransferase to synthesize GalNAc (Fuc<alpha>1, 2)<alpha>1, 3GAL or Gal (Fuc<alpha>1, 2)<alpha>1, 3Gal glycosidic, fucose is removed to synthesize target oligosaccharide containing a GalNAc<alpha>1, 3Gal or Gal<alpha>1, 3Gal glycosidic bond. The method combines high region selectivity and high efficiency of enzymatic synthesis together and realizes synthesis of a target molecule with a higher yield. The glycosyltransferase, glycoribosidase and glucokinse used in the method are all derived from prokaryotes, and the method has the advantages of high protein expression quantity, wide substrate adaptability, high catalysis efficiency and the like and can be applied to mass preparation.