The invention provides a 3D printing method and device for manufacturing a micro-fluidic
chip by utilizing a nanometer
fiber paper base laminate, relating to micro-fluidic chips. The 3D printing method comprises the following steps: 1) depositing a nanometer
fiber paper base on a collecting plate; 2) moving the collecting plate into a hydrophobic material jet printing area, heating for maintaining the hydrophobic material in a
sprayer to be in a
molten state, adding into an
electric field, and downward jetting the hydrophobic material
fiber, thereby forming a hydrophobic material fiber pattern; 3) designing each layer of hydrophobic material fiber pattern, and constructing the structure of the micro-fluidic
chip in a 3D printing manner of continuously overlapping the nanometer fiber paper base and the hydrophobic material fiber pattern; and 4) heating the collecting plate, permeating each layer of hydrophobic material into the nanometer fiber paper base, constructing a micro-fluidic control structure, and finally forming the micro-fluidic
chip with the 3D structure on the nanometer fiber paper base. The printing device is provided with a
direct current power supply, a two-dimension jet printing motion platform, a feeding pump, a
spinning needle, a
liquid storage needle drum, a jet, an auxiliary power supply, a
temperature control device, a conductive substrate, an insulating plate, the collecting plate and a lifting platform.