33results about How to "Suitable for separation and purification" patented technology

The invention relates to a method for extracting purified chlorogenic acid from jerusalem artichoke leaves. The method comprises the following steps: by adopting jerusalem artichoke leaves as raw materials, performing heating reflux or ultrasonic extraction, and performing solid-liquid separation to obtain a chlorogenic acid extracting liquid, then performing membrane separation and membrane concentration, passing through a macroporous resin column, feeding an impurity washing liquid for impurity washing, performing elution by using an eluent until high performance liquid chromatography (HPLC) detects that no chlorogenic acid flows out, and collecting the eluent, performing reduced pressure concentration on the eluent, performing decoloration by using activated carbon, and then performing spray-drying to obtain high-purity chlorogenic acid with the chlorogenic acid purity of more than 94%. The method disclosed by the invention is large in sample treatment quantity and simple to operate, and is suitable for industrial production; the defects that traditional Chinese medicine resources such as honeysuckl and the like are used as chlorogenic acid extraction raw materials can be overcome, the purpose of changing waste into wealth can be achieved, and a new source for extracting chlorogenic acid can be provided; and the method has relatively good economic, social and environmental benefits.

The invention provides a multi-channel comprehensive two-dimensional high-performance liquid chromatograph with a multifunctional switching valve. The multi-channel comprehensive two-dimensional high-performance liquid chromatograph comprises a one-dimensional chromatographic separation system and a multi-channel two-dimensional chromatographic separation system and has the advantages that by the aid of a multi-channel two-dimensional separation system, two-dimensional separation is achieved by the aid of multiple external chromatographic columns, selectable types of stationary phases are more, separation efficiency is high, separation effect is good, total components of complicated samples can be separated and analyzed, mobile phases of each channel are independently controlled, separation processes can be accurately controlled, separation effect is improved, two-dimensional separation is achieved by the aid of a plurality of two-dimensional chromatographic columns, limit of the size of one-dimensional chromatographic columns and flow speed of the mobile phases is omitted, and the chromatograph can be used for preparative separation of large samples.

The invention relates to a dynamic purification regenerator of a harmful solvent. The regenerator can carry out gas-liquid-solid three-phase separation. The dynamic purification regenerator of the harmful solvent comprises a tower head condenser, an upper fractionation section, a tower middle feed section, a lower fractionation section and a tower kettle which are sequentially in ground joint from top to bottom, wherein the tower head condenser is connected with a pressure stabilizing opening communicated with the atmosphere, a condenser pipe combining a ball shape and a snake shape is arranged in the tower head condenser, the pipe wall of the condenser is connected with a condensed water outlet and a condensed water inlet, a vapour-liquid distributor enabling ascending vapour to contact with descending condensate is arranged below the condenser pipe combining the ball shape and the snake shape, the pipe wall of the condenser is connected with an electromagnetic valve of the condensed water outlet and a tower top discharge hole used for extracting a light component at the tower top, the electromagnetic valve controls the flow rate of the reflux ratio, a speed reducer drives an evaporation bottle to rotate, the evaporation bottle is connected with the feed section by flexible connection and is heated by adopting a constant-temperature water bath, and the tower kettle is heated by adopting an electric heating sleeve. The invention has the characteristics of small equipment height, simple and convenient operation, high efficiency, high separation speed, energy saving and the like and is suitable for the separation and the purification of a conventional mixed solvent.

The invention discloses a recycling preparative HPLC (high performance liquid chromatograph) provided with a multi-ported valve as well as a method used for preparation, separation and purification. The recycling preparative HPLC provided with the multi-ported valve comprises a liquid accumulator M, a high-pressure pump P, a sampler S, chromatographic columns C1 and C2, the multi-ported valve, detectors D1 and D2, three-way switching valves T1 and T2, liquid pipelines and the like. The recycling preparative HPLC has the outstanding effects as follows: 1, to-be-separated mixed components are circularly separated only in the two chromatographic columns and are not required for circulation through the high-pressure pump, chromatographic peak broadening is light, the separation efficiency is high, and the separation effect is good; 2, a system is provided with two detectors, peak cutting and circular separation can be accurately controlled, and the separation effect is improved; 3, eluent without the separated components is completely recycled, the consumption of eluent is greatly reduced, and the separation cost is very low.

The invention belongs to the technical field of separation and purification of effective components of plant leaves, and in particular relates to a method for separating and purifying plant leaf anthocyanins with mixed adsorption resins. The present invention mixes non-polar and medium-polar resins according to a certain mass ratio, and adjusts the hydrophobic force of the resin by regulating the content of different polar components in the resin skeleton, thereby weakening the adsorption force of the resin and anthocyanin, and at the same time Without affecting the adsorption capacity of the resin to impurities, a mixed macroporous resin filter body suitable for the separation and purification of plant leaf anthocyanins is finally formed.

The invention relates to an online low-pressure, middle-pressure and high-pressure combined two-dimension preparation liquid chromatographic system. Two one-dimension low-pressure, middle-pressure and high-pressure preparation liquid chromatographic systems are organically combined through a six-way valve and software. Pressure used by a second dimension system is not lower than pressure used by a first dimension separation system. When the first dimension system and the second dimension system are low-pressure or middle-pressure preparation liquid chromatographic systems, through switching the six-way valve, target compound directly enters a second dimension chromatographic column for second dimension separation after first dimension separation; and when pressure used by a second dimension separation system is higher than pressure used by the first dimension separation system, a trapping column is connected on the six-way valve, the target compound is adsorbed on the head of the trapping column after the first dimension separation, and then the target compound enters the second dimension chromatographic column for second dimension separation. The invention has the advantages thatthe contradiction between sample injection volume and separation capacity is solved, the large sample injection volume is ensured and the separation capacity of the preparation chromatographic systemis greatly improved; the system structure is simple and the establishment is easy; the operation is simple and convenient and the automation can be easily realized; and the application scope is wide.

This invention relates to extraction process of camel colostrum immunoglobulin IgA and IgG, belongs to biochemistic product field. The camel colostrum is the most prolific natural source of immunoglobulin, and is one of the biotic resources that has most concentrated immune factor. This invention supplies a craft of using gradient elution to extract IgA and using ultrafiltration and metal chelatechromatography to extract IgG. By test has show that in product IgA basicly has no other impurity, purity of IgG can reach 97 percent and above.

The invention discloses a polymer microsphere with super large pores and a high cross-linking degree and a preparation method thereof, comprising: dissolving a stabilizer and a surfactant in deionized water to prepare an aqueous phase solution. The vinyl monomer and the divinyl crosslinking agent are dissolved in the porogen solution, and after nitrogen filling and oxygen exhausting, an atom transfer radical polymerization initiator system is added to prepare an oil phase solution. Under the conditions of filling nitrogen and keeping stirring, the oil phase solution is added to the water phase solution, and the temperature rise polymerization reaction is carried out. The reaction temperature is 50-100 ° C, and the reaction time is 13-24 hours; Filter and wash to remove unreacted residues to obtain polymer microspheres. The invention can not only make the average pore size of the polymer microspheres controllable within 980-3900nm, but also does not need to use a large amount of surfactant, and does not need complicated cleaning process after the preparation, and has good application prospects in the field of rapid separation and purification of biological macromolecules. It is especially suitable for the separation and purification of large-sized vaccine particles.

The invention provides a preparation supercritical fluid chromatographic instrument with a multi-way valve and a separation and purification method of the preparation supercritical fluid chromatographic instrument. Complicated mixed components to be separated are efficiently separated through on / off switching of the multi-way valve by adopting a separation mode including pre-separation performed by a pre-separation column, capturing performed by a plurality of capturing columns and separation performed by a plurality of main separation columns; the separated components are collected in a collector through the on / off switching of the valve, and a mobile phase without the separated components is returned back to a system for cyclic utilization. The supercritical fluid chromatographic instrument provided by the invention is simple in design, good in separation effect and extremely high in practicability, and is applicable to separation and purification of various types of complicated mixtures.

The invention discloses a method for extracting, purifying, and inspecting astragalus in Beiqi mushrooms, and relates to the field of extraction, purification, and inspection of active substances in the Beiqi mushrooms. The method comprises the following steps of: taking 70% of ethanol as an extracting agent, and carrying out auxiliary treatment through a microwave ultrasonic auxiliary extractor to obtain an astragalus crude extracting solution; taking a mixed solution of chloroform, methanol, ethyl acetate, and water as a developing solvent, and separating and purifying astragaloside, astragalus saponin I, II, and III through thin-layer chromatography; using chromatography methanol and ultrapure water as a mobile phase, and accurately determining sample molecular mass information (a mass-to-charge ratio of molecular ions or fragment ions) with a high resolution mass spectrometer; detecting a functional group of the sample by infrared, and analyzing the molecular structure of the sample; and determining a rotation degree, a specific rotation degree, and a melting point of the sample. According to the method for extracting, purifying, and inspecting astragalus in Beiqi mushroom, microgram amount of separation analysis to gram amount of separation preparation can be achieved by using TLC-MS-IR series technology to detect astragalus in the Beiqi mushrooms; and the method is simpleto operate, short in time and high in efficiency.

The invention discloses a separation and purification preparation chromatograph and a method for preparation, separation and purification.By means of switching-on and switching-off combination of multiple valves, a complex mixture to be separated is effectively separated through the separation mode that the complex mixture is preseparated by a preseparation column, captured by multiple capturing columns and then separated by multiple main separating columns, separated components are collected by a collector through switching-on and switching-off switching of the valves, and a mobile phase which does not contain the separated components returns back to systemic circulation for use.