Multichannel two-dimensional chromatograph and method for two-dimensional chromatographic separation

Abstract

The invention discloses a multichannel two-dimensional chromatograph and a method for two-dimensional chromatographic separation. The multichannel two-dimensional chromatograph consists of a one-dimensional chromatographic separation system and a multichannel two-dimensional chromatographic separation system, wherein the one-dimensional chromatographic separation system consists of a one-dimensional chromatographic flowing phase liquid accumulator M, a one-dimensional chromatographic high-pressure pump P, a one-dimensional chromatographic sample valve V, a one-dimensional chromatographic column C and a one-dimensional chromatographic detector D; the multichannel two-dimensional chromatographic separation system consists of multiple two-dimensional chromatographic flowing phase liquid accumulators M1 to M8, multiple two-dimensional chromatographic high-pressure pumps P1 to P8, multiple two-dimensional chromatographic columns C1 to C8, multiple two-dimensional chromatographic detectors D1 to D8 and a fraction collector. The multichannel two-dimensional chromatograph and the method are high in separation efficiency and practicability, and particularly applied to all-component separation of complex systems such as traditional Chinese medicaments and body fluids and separation of difficult-to-separate substances such as structurally similar compounds.

Description

technical field [0001] The application relates to a multi-channel two-dimensional chromatographic instrument and a method for two-dimensional chromatographic separation, belonging to the technical field of instrument and equipment manufacturing, and is a high-efficiency chromatographic separation and analysis device. Background technique [0002] The chromatographic method has the characteristics of high efficiency, rapidity and wide application. It has developed into a key technology to solve various separation and analysis problems in the research of organic substances. It is widely used in the fields of pharmacy, life science, medicine, food science, petrochemical industry and environmental protection. be widely used. The usual chromatograph is composed of mobile phase storage, mobile phase pump, sample injector, chromatographic column, detector, recorder, etc. During the application process, a chromatographic column is often used for separation, that is, one-dimensional ...

AI Technical Summary

Problems solved by technology

In existing instruments, limited by the interface mode, the one-dimensional flow rate is required to be very small, and the length of the two-dimensional separation column must be very short. It is difficult to use a conventional chromatographic column as a two-dimensional chromatographic column for separation. restricted

For large-capacity preparative separation, the existing technology cannot meet the demand, and cannot be directly applied to the separation and preparation of components

In addition, the existing two-dimensional technology uses a one-dimensional chromatographic column and a two-dimensional chromatographic column for separation, and at most two stationary phase chromatographic columns can be used for separation at a time, which is difficult to meet the separation needs of complex samples

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