A kind of high cross-linking degree polymer microspheres with super macropores and preparation method thereof

Abstract

The invention discloses a polymer microsphere with super large pores and a high cross-linking degree and a preparation method thereof, comprising: dissolving a stabilizer and a surfactant in deionized water to prepare an aqueous phase solution. The vinyl monomer and the divinyl crosslinking agent are dissolved in the porogen solution, and after nitrogen filling and oxygen exhausting, an atom transfer radical polymerization initiator system is added to prepare an oil phase solution. Under the conditions of filling nitrogen and keeping stirring, the oil phase solution is added to the water phase solution, and the temperature rise polymerization reaction is carried out. The reaction temperature is 50-100 ° C, and the reaction time is 13-24 hours; Filter and wash to remove unreacted residues to obtain polymer microspheres. The invention can not only make the average pore size of the polymer microspheres controllable within 980-3900nm, but also does not need to use a large amount of surfactant, and does not need complicated cleaning process after the preparation, and has good application prospects in the field of rapid separation and purification of biological macromolecules. It is especially suitable for the separation and purification of large-sized vaccine particles.

Description

technical field [0001] The invention relates to the field of polymer microsphere materials, in particular to a polymer microsphere with super large pores and a high crosslinking degree and a preparation method thereof. Background technique [0002] Polymer microspheres refer to polymer particles with a spherical shape and a particle size ranging from tens of nanometers to hundreds of microns. As a biochemical separation medium, polymer microspheres have the advantages of high mechanical strength, stable chemical properties, good acid and alkali resistance, and can be operated under high pressure. Therefore, polymer microspheres have good prospects in the application field of chromatographic media. [0003] Since the separation objects in biochemical separation applications are biomacromolecules, and these biomacromolecules have the characteristics of large molecular size and complex structure, etc., they are easily inactivated and denatured during separation and purification...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the existing suspension polymerization method is difficult to prepare polymer microspheres above the micron scale, and other methods need to use a large amount of surfactants, and a complicated cleaning process is required after the preparation, and the obtained polymer microspheres have a low degree of crosslinking and other technical problems, the present invention provides a highly cross-linked polymer microsphere with super macropores and a preparation method thereof, which not only can make the average pore diameter of the prepared polymer microsphere controllable within the range of 980-3900nm , the mass transfer rate of large-sized biomolecules is greatly improved, and the polymerization temperature range is wide, the preparation time is short, there is no need to use a large amount of surfactant in the preparation process, and no complicated cleaning process is required after the preparation. At the same time, the prepared polymer micro The ball has a high degree of cross-linking, can withstand the operating pressure of 3-10MPa, and can continue to trigger ATRP polymerization (Atom Transfer Radical Polymerization, atom transfer radical), which is convenient for further derivation into various functional media, so it can be used in the rapid separation and purification of biological macromolecules The field has good application prospects, especially suitable for the separation and purification of large-sized vaccine particles

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