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A separation and purification preparative chromatograph and method for preparation, separation and purification

A technology of separation and purification and chromatograph, which is applied in the field of equipment manufacturing, can solve the problems of unsatisfactory product purity and increased separation cost, and achieve the effect of improved separation effect, low cost and good separation effect

Inactive Publication Date: 2017-12-26
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

When performing sample separation, it is difficult to obtain the ideal separation effect for complex samples, and the purity of the product obtained by one-dimensional separation is sometimes unsatisfactory. It is necessary to collect chromatographic fractions and recover the mobile phase before performing a second sample separation.
In addition, when the sample is separated, a large amount of eluent needs to be used, which also increases the cost of separation

Method used

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  • A separation and purification preparative chromatograph and method for preparation, separation and purification
  • A separation and purification preparative chromatograph and method for preparation, separation and purification

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Embodiment Construction

[0025] The present invention will be described in detail below in conjunction with the embodiments and drawings, but the scope of protection is not limited thereto.

[0026] Examples such as figure 1 As shown, a separation and purification preparation chromatograph and a method for preparation, separation and purification, the separation and purification preparation chromatograph consists of fluid delivery pumps P1, P2, P3, P4, sampling system Cs, pre-separation column C1, detector D1, Valve V (1-32), trapping column system C2, detector D2, back pressure valve B1, separation column system C3, detector D3, component collection system S, back pressure valve B2, flow pipeline, etc.

[0027]Fluid 1 is transported by delivery pump P1, and fluid 2 is delivered by delivery pump P2. After the two are combined and mixed, they pass through the sample injection system Cs, and the samples carried in Cs enter the pre-separation column C1 for pre-separation, and the effluent of the pre-sepa...

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Abstract

The invention discloses a separation and purification preparation chromatograph and a method for preparation, separation and purification.By means of switching-on and switching-off combination of multiple valves, a complex mixture to be separated is effectively separated through the separation mode that the complex mixture is preseparated by a preseparation column, captured by multiple capturing columns and then separated by multiple main separating columns, separated components are collected by a collector through switching-on and switching-off switching of the valves, and a mobile phase which does not contain the separated components returns back to systemic circulation for use.

Description

technical field [0001] The invention relates to a separation and purification preparation chromatograph and a method for preparation, separation and purification, which belong to the technical field of instrument and equipment manufacturing, and are a chromatographic separation and purification device. Background technique [0002] At present, chromatography has developed into an important separation technology widely used in the fields of chemistry, life science and pharmaceuticals. Preparative chromatography refers to the use of chromatographic techniques to prepare pure substances, that is, to separate and collect one or more chromatographically pure substances. The concept of "preparation" in preparative chromatography refers to obtaining sufficient quantities of a single compound for research and other purposes. The emergence of preparative chromatography has established a link between chromatography technology and economic interests. The amount of preparation and the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 柳仁民孙爱玲
Owner LIAOCHENG UNIV
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