A multi-channel comprehensive two-dimensional high-performance liquid chromatograph with a multi-functional switching valve and its application

Abstract

The invention provides a multi-channel comprehensive two-dimensional high-performance liquid chromatograph with a multifunctional switching valve. The multi-channel comprehensive two-dimensional high-performance liquid chromatograph comprises a one-dimensional chromatographic separation system and a multi-channel two-dimensional chromatographic separation system and has the advantages that by the aid of a multi-channel two-dimensional separation system, two-dimensional separation is achieved by the aid of multiple external chromatographic columns, selectable types of stationary phases are more, separation efficiency is high, separation effect is good, total components of complicated samples can be separated and analyzed, mobile phases of each channel are independently controlled, separation processes can be accurately controlled, separation effect is improved, two-dimensional separation is achieved by the aid of a plurality of two-dimensional chromatographic columns, limit of the size of one-dimensional chromatographic columns and flow speed of the mobile phases is omitted, and the chromatograph can be used for preparative separation of large samples.

Description

technical field [0001] The invention relates to a multi-channel comprehensive two-dimensional high-performance liquid chromatograph and a method for comprehensive two-dimensional chromatographic separation, belongs to the technical field of instrument and equipment manufacturing, and is a high-performance liquid chromatographic separation and analysis device. Background technique [0002] High performance liquid chromatography has the characteristics of high efficiency, rapidity and wide application, and has been widely used in the fields of pharmacy, life science, medicine, food science, petrochemical industry and environmental protection. The usual high-performance liquid chromatography is composed of a liquid reservoir, a high-pressure pump, a sampler, a chromatographic column, a detector, a recorder, etc. During the application process, a chromatographic column is often used for separation, that is, one-dimensional chromatographic separation. In the one-dimensional chrom...

AI Technical Summary

Problems solved by technology

Limited by the interface mode, the one-dimensional flow rate is required to be small, and the two-dimensional separation column should be short, which limits the entire separation effect

For large-capacity preparation and separation, the existing technology cannot meet the demand, and cannot be directly applied to the separation and preparation of components

In addition, the existing comprehensive two-dimensional technology uses one one-dimensional chromatographic column and one two-dimensional chromatographic column for separation, and can only use at most two stationary phase chromatographic columns for combined separation at a time, which is difficult to meet the separation needs of complex samples

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