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A method for extracting and purifying chlorogenic acid from Jerusalem artichoke leaves

A technology of chlorogenic acid and Jerusalem artichoke, which is applied in chemical instruments and methods, preparation of organic compounds, separation/purification of carboxylic acid esters, etc., can solve the problems of unsecured safety, destruction of ecological environment, limited raw materials, etc. Achieve the effect of shortening post-processing time, less control parameters, and fast adsorption rate

Active Publication Date: 2016-03-16
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, China mainly extracts chlorogenic acid from high-priced medicinal resources such as honeysuckle and Eucommia ulmoides. The raw materials are limited and expensive, so it is urgent to find alternative sources for extracting chlorogenic acid
Chinese patents 200710098997.9 and 200810244181.7 extract and separate chlorogenic acid from Eupatorium adenophorum and Solidago, but Eupatorium adenophorum and Solidago have strong environmental invasiveness and are easy to cause damage to the ecological environment
Chinese patent 201210531489.6 extracts and separates chlorogenic acid from tobacco, but tobacco contains many harmful substances, and its safety cannot be guaranteed

Method used

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  • A method for extracting and purifying chlorogenic acid from Jerusalem artichoke leaves
  • A method for extracting and purifying chlorogenic acid from Jerusalem artichoke leaves
  • A method for extracting and purifying chlorogenic acid from Jerusalem artichoke leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Accurately weigh the above 100g of Jerusalem artichoke leaf coarse powder into the extractor, add 500mL of methanol aqueous solution with a volume fraction of 10%, and heat and reflux at 100°C for extraction twice, each time for 1h. Filter and combine the extracts. The extract is first passed through a hollow fiber ultrafiltration membrane with a molecular weight cut off of 1000 to remove macromolecular impurities, and then the extract is concentrated to 10% of the original volume fraction with a nanofiltration membrane with a molecular weight cut off of 200. The concentrated solution is put on the ADS-7 macroporous resin column at a flow rate of 5BV / h. After the dynamic adsorption is saturated, the macroporous resin column is rinsed with an aqueous hydrochloric acid solution with pH=5-6 at a flow rate of 5BV / h until the effluent is colorless. , and then elute with 40% ethanol at a flow rate of 5BV / h, collect the ethanol eluate, concentrate it under reduced pressure to ...

Embodiment 2

[0034] Accurately weigh 500g of Jerusalem artichoke leaf meal and put it into an extractor, add 3000mL of 30% ethanol aqueous solution, heat and reflux at 80°C to extract twice, each time for 2.5h. Filter and combine the extracts. The extract is first passed through a hollow fiber ultrafiltration membrane with a molecular weight cut off of 1000 to remove macromolecular impurities, and then the extract is concentrated to 10% of the original volume fraction with a nanofiltration membrane with a molecular weight cut off of 200. The concentrated solution is put on the DM130 macroporous resin column at a flow rate of 4BV / h. After the dynamic adsorption is saturated, the macroporous resin column is rinsed with a hydrochloric acid aqueous solution with pH=5-6 at a flow rate of 4BV / h until the effluent is colorless, and then Use ethanol with a volume fraction of 60% to elute at a flow rate of 6BV / h, collect the ethanol eluent, concentrate it under reduced pressure to a paste, and then...

Embodiment 3

[0036] Accurately weigh 1000g of Jerusalem artichoke leaf coarse powder and put it into an extractor, add 8000mL of 60% ethanol aqueous solution, heat and reflux at 50°C to extract twice, each time for 3h. Filter and combine the extracts. The extract is first passed through a hollow fiber ultrafiltration membrane with a molecular weight cut off of 1000 to remove macromolecular impurities, and then the extract is concentrated to 10% of the original volume fraction with a nanofiltration membrane with a molecular weight cut off of 200. The concentrated solution is applied to the HPD-600 macroporous resin column at a flow rate of 3BV / h. After the dynamic adsorption is saturated, the macroporous resin column is rinsed with an aqueous hydrochloric acid solution with pH=5-6 at a flow rate of 3BV / h until the effluent is colorless. , and then eluted with 60% ethanol at a flow rate of 8BV / h, collected the ethanol eluate, concentrated it under reduced pressure to a paste, and then dissol...

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Abstract

The invention relates to a method for extracting purified chlorogenic acid from jerusalem artichoke leaves. The method comprises the following steps: by adopting jerusalem artichoke leaves as raw materials, performing heating reflux or ultrasonic extraction, and performing solid-liquid separation to obtain a chlorogenic acid extracting liquid, then performing membrane separation and membrane concentration, passing through a macroporous resin column, feeding an impurity washing liquid for impurity washing, performing elution by using an eluent until high performance liquid chromatography (HPLC) detects that no chlorogenic acid flows out, and collecting the eluent, performing reduced pressure concentration on the eluent, performing decoloration by using activated carbon, and then performing spray-drying to obtain high-purity chlorogenic acid with the chlorogenic acid purity of more than 94%. The method disclosed by the invention is large in sample treatment quantity and simple to operate, and is suitable for industrial production; the defects that traditional Chinese medicine resources such as honeysuckl and the like are used as chlorogenic acid extraction raw materials can be overcome, the purpose of changing waste into wealth can be achieved, and a new source for extracting chlorogenic acid can be provided; and the method has relatively good economic, social and environmental benefits.

Description

technical field [0001] The invention relates to a method for extracting and purifying chlorogenic acid, in particular to a method for extracting and purifying chlorogenic acid from Jerusalem artichoke leaves. Background technique [0002] Jerusalem artichoke (Helianthus tuberosus L.) is commonly known as Jerusalem artichoke, devil ginger. Native to North America, it is a perennial herbaceous plant of the genus Sunflower in the Compositae family. Because Jerusalem artichoke has strong environmental adaptability, has the characteristics of cold and drought resistance, wind and sand resistance, strong reproductive ability, and easy planting and management, it can be planted in beaches, deserts and other areas. It is a kind of plant that can be used to improve land desertification and salinization. As an economic plant, the extensive planting and comprehensive development and utilization of Jerusalem artichoke are of great value to improving the ecological environment and increa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/732C07C67/48C07C67/56
CPCC07C67/48C07C67/56C07C2601/14C07C69/732
Inventor 夏传海孙鹏程方圣涛
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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