The invention belongs to the technical field of
gene detection, and particularly relates to a low-frequency
mutation detection method based on a
snapback primer technology. The method comprises: designing a
snapback primer, preparing
a DNA template, preparing a detection
system, setting a reaction procedure and determining a Ts value, judging a result, and other steps. According to the present invention, the detection method is the
snapback ARMS and snapback primer HRM combined low-frequency
mutation detection technology, such that the detection sensitivity of 1:10000 or even higher is achieved, clinical requirements of
pollution resistance, high sensitivity, strong specificity, easy performing and result judgment on the
mutation detection technology are met, and the method can be used for
gene screening,
genotyping (SNP,
deletion mutation,
insertion mutation) and other fields of various types of tissues including
fresh tissue samples,
whole blood, serum, tissue slices and other samples.