75results about How to "Shorten the enrichment time" patented technology

The invention discloses a germinated whole grain and a preparation method thereof, and belongs to the technical field of deep processing of grains. The whole grain is subjected to ultrasonic washing, high-voltage pulsed electric field treatment, biological enzymolysis or combined high-voltage pulsed electric field and biological enzymolysis treatment in a soaking process, and a segmented soaking and germination manner for germination is adopted, so that the sensory quality, germination yield and quality of a germinated whole grain product are improved, and germination loss is reduced. The preparation method is simple, low in soaking and germination temperature, short in cycle, energy-saving and environment-friendly. The content of functional, nutritional and bioactive substances in the germinated whole grain product is remarkably increased, and food safety is remarkably improved. For example, for brown rice, germinated brown rice prepared by the preparation method has gamma-aminobutyric acid content of 303.8 to 312.6 mg / 100 g, glutathione content of 16.8 to 18.3 mg / 100 g, IP6 content of 460.8 to 484.4 mg / 100 g and dietary fiber content of 3.5 to 3.6 mg / 100 g.

The invention discloses a method for synchronously detecting various pathogens in raw milk and a detection reagent kit, and relates to a method for detecting bacteria in the raw milk and a detection reagent kit. The invention solves the problems that the prior method for detecting the pathogens in the raw milk fails to synchronously detect the pathogens, has long time and high cost, or is not suitable to detect cow milk. The method for synchronously detecting the various pathogens in the raw milk comprises the following steps: 1. removing the fat in the raw milk to be detected; 2. filtering a sample which is subjected to thermal insulation in step 1; 3. collecting an eluent; and 4. performing the multiplex PCR, and analyzing a gel imaging system to obtain a detection result. The reagent kit consists of a Taq enzyme solution, a multiplex PCR reaction liquid, a negative control solution, and a positive control solution. The method has the advantages of short time, low cost, adaptability of detecting the cow milk and accurate detection results. The reagent kit is convenient to use.

The invention discloses a bio-floating bed for in-situ reduction of water body heavy metal pollution. The bio-floating bed comprises a housing (2), a filler bed (3) accommodated in the housing (2), hyper-accumulator plants (1) which grow on the filler bed (3) and magnetotactic bacteria accumulated in the filler bed (3). The invention also discloses a method for in-situ reduction of water body heavy metal pollution by virtue of the bio-floating bed. The method comprises the following steps: a, arranging the bio-floating bed in the water body polluted by heavy metal; b, accumulating the heavy metal by virtue of chlorella, magnetotactic bacteria and the hyper-accumulator plants; c, regularly harvesting the hyper-accumulator plants to remove the accumulated heavy metal. By the bio-floating bed and the method disclosed by the invention, the heavy metal in the water body can be cheaply, efficiently and harmlessly removed.

The invention relates to a method for enriching vitamin K2 in natto bacillus fermentation liquid. The method comprises the following steps of step 1, performing centrifugation on the natto bacillus fermentation liquid, and collecting supernatant; step 2, adding a flocculant and a filter aid to the supernatant obtained in the step 1, then performing stirring, performing centrifugation, and collecting supernatant; step 3, adding Fe3O4@SiO2-G nanometer microspheres which are reduced to the supernatant obtained in the step 2, regulating the pH to 6.0-8.0, then performing stirring treatment under the condition of 20-35 DEG C for 10-30min, and then collecting magnetic materials after adsorption; and step 4, adding hexane to the magnetic materials after adsorption, performing ultrasonic parsing for 5-20min, then externally applying a magnet, collecting magnetic nanometer materials, pouring out organic phase, and performing filtering, wherein filtrate is a hexane solution rich in vitamin K2. According to the method for enriching vitamin K2 in natto bacillus fermentation liquid, the VK2 in the natto bacillus fermentation liquid can be quickly and efficiently enriched. The method has the advantages that the specific steps are simple, the materials are low in price, and can be reused, and the enrichment effect is efficient.

The invention discloses high-solubility compound protein powder and a preparation method thereof. Soybean protein isolate, whey protein concentrate and phospholipid are taken as main raw materials, stevia rebaudiana is processed into a stevia rebaudiana extract at low temperature, the effective ingredients and the bioactive ingredients of the stevia rebaudiana can be maximumly retained, the extraction rate is high, the flavor is pure, the color and cluster are natural, no licorice taste exists, the stevia rebaudiana extract is scientifically compounded with main materials, antifreeze polypeptides, dietary fiber flour, bee pupa hydrolysates and wheat malt powder are added, and then the compound protein powder which is appropriate in mouthfeel, good in solubility, comprehensive in nutrition, high in bioactivity and wide in suitable crowd is prepared. According to the preparation method, a non-thermal processing technique is adopted, the working efficiency is high, energy consumption is little, environmental friendliness is achieved, no pollution is generated, introduction of food additives and chemical auxiliaries is reduced, the food safety is effectively improved, the product quality is improved, the production cost is reduced, the low-carbon production objective is achieved, and large-scale and industrialized development can be achieved.

The invention discloses a functional compound protein composition and a preparation method thereof. According to the functional compound protein composition and the preparation method thereof, soybean protein isolate, whey protein concentrate and phospholipids are utilized as main raw materials; low-temperature processing is performed on stevia rebaudiana so as to prepare a stevia rebaudiana extract to preserve active ingredients and biologically active ingredients of the stevia rebaudiana to the maximum extents; the prepared stevia rebaudiana extract is high in extraction rate, pure in flavor, natural in color and luster, and free of liquorice smell; the stevia rebaudiana extract and the main raw materials are scientifically compounded, and antifreeze polypeptides, dietary fiber powder, bee pupae enzymatic hydrolysate and wheat malt flour are added, so that the functional compound protein composition is prepared; and the prepared functional compound protein composition is appropriate in taste, good in solubility, whole in nutrition, good in biological activities and suitable for a wide range of people. The preparation method adopts a non-thermal processing technology, so that the preparation method is high in work efficiency, small in energy consumption, friendly to the environment and free of pollution; moreover, the preparation method reduces introductions of food additives and chemical additives, so that safety of the foods is effectively improved, qualities of the foods are improved and production costs are reduced. The preparation method of the functional compound protein composition realizes low-carbon production target, and is capable of realizing scale development and industrialized development.

The invention discloses a wheat malt composite powder and a preparation method thereof.Wheat malt powder with a natural color, a natural flavor and a natural taste is scientifically compounded with functional products such as lactobacillus plantarum powder, modified dietary fibers, ferment powder and oligosaccharide, so that the trophism, the food safety and the healthcare function of the wheat malt composite powder are effectively improved, and the probiotic property and the quality stability of the wheat malt composite powder are also significantly improved.The wheat malt composite powder is prepared from simple components and is natural in color, flavor and taste, high in nutritive value, strong in healthcare function, high in food safety and long in shelf life, the content of bioactive substances is high, the viable count of probiotics is high, the probiotic property is high, and complete varieties of functional metabolites of probiotics are provided with a high content.Ultrasonic cleaning, instant microwave puffing and germ killing, high-voltage pulse processing, combined biological enzymolysis and soaking and germination accelerating are adopted in the wheat malt preparation process.The soaking and germination time is short, the germination rate is high, the content of bioactive substances is high, the production efficiency is high, environmental pollution is avoided, and environmental friendliness is achieved.

The present invention discloses a silurus asotus feed phagostimulant and a preparation method thereof, and belongs to the technical field of feed additive preparations. Based on nutritional requirements of silurus asotus and water body environment, and combining food intake characteristics, a formula of raw materials is optimized. The silurus asotus feed phagostimulant scientifically combines compound flavor peptides greatly improving palatability and feed utilization rate, having a relatively strong anti-oxidation effect and eliminating free radicals; an accharomyces cerevisiae culture having functional characteristics of being strong in nutrition, biological activity, oxidation resistance, etc.; a high-calcium cerasus humilis extract effectively supplementing calcium, iron and soluble fibers, having a strong embedding effect and prolonging a shelf life; artemisia carvifolia seed powder significantly improving antifreeze effects and stability, improving water resistance and gloss, and enhancing and promoting palatability; and wheat malt powder providing rich nutrients, bioactive substances and plant enzyme sources. Finally, the prepared silurus asotus feed phagostimulant is strong in phagostimulant and good in production performances.

The invention discloses a sprouting whole grain with high sprouting rate and a preparation method of the sprouting whole grain, belonging to the technical field of deep processing of grains. A soaking process is subjected to ultrasonic cleaning, as well as high-pressure pulsed electric field treatment or biological enzymolysis or the combination of the high-pressure pulsed electric field treatment and the biological enzymolysis, and a manner of soaking and sprouting in different stages is adopted, so that the sensing quality and the sprouting yield and quality of the sprouting whole grain product are improved, and the sprouting loss is reduced. The preparation method is simple, the temperature for soaking and sprouting is low, and the period is short, so that energy source is saved, and the preparation method is environment-friendly. The contents of functional, nutritious and biological active substances in the sprouting whole grain product as well as the food safety are significantly improved. For example, in sprouting coarse rice prepared through the preparation method disclosed in the invention, the content of gamma-aminobutyric acid is 303.8-312.6 mg / 100g, the content of glutathione is 16.8-18.3 mg / 100g, the content of inositol pexaphosphate (IP6) is 460.8-484.4 mg / 100g, and the content of dietary fiber is 3.5-3.6 mg / 100g.

The invention discloses a composite protein powder capable of improving immunity and a preparation method thereof. Using soybean protein isolate, concentrated whey protein and phospholipids as main raw materials, stevia is processed into stevia extract at low temperature, which can retain Active ingredients and bioactive ingredients of stevia, high extraction rate, pure flavor, natural color, no licorice taste, scientifically compounded with main ingredients, and added antifreeze polypeptide, dietary fiber powder, bee chrysalis enzymatic hydrolyzate, wheat germ Powder, to prepare a compound protein powder with suitable taste, good solubility, comprehensive nutrition, strong biological activity, suitable for a wide range of people, and capable of improving immunity. The preparation method adopts non-thermal processing technology, which has high work efficiency, low energy consumption, environmental friendliness, no pollution, reduces the introduction of food additives and chemical additives, effectively improves food safety, improves product quality and reduces production costs , achieved the goal of low-carbon production, and can be scaled and industrialized.

The invention discloses a catfish feed compound enzyme and a preparation method thereof. The preparation method of the catfish feed compound enzyme is characterized by taking nutritional requirement of the catfish and the water environment as the basis, optimizing the raw material formula, combining digestion characteristics of the catfish and scientifically compounding a wine making yeast culture material with functional characteristics such as high nutrition performance, high biological activity, high oxidation resistance and the like, a calcium fruit extract capable of effectively supplementing calcium, iron and soluble fiber, providing powerful embedding effect and prolonging the quality guarantee period of the feed compound enzyme, wormwood seed powder capable of obviously improving anti-freezing effect and stability of the feed compound enzyme, improving water resistance and glossiness of the catfish feed and reducing powder content in the feed, a palatable agent capable of greatly improving palatability and digestion rate of the feed, and wheat malt flour capable of providing rich nutrient materials, biological active materials and plant enzyme sources to finally obtain the catfish feed compound enzyme capable of obviously enhancing digestion rate and parturition performance of the catfish, reducing the feed coefficient, preventing water pollution and maintaining high water quality.

The invention discloses a high-yield catfish feed attractant and a preparation method thereof and belongs to the technical field of feed additive preparation. The high-yield catfish feed attractant and the preparation method thereof have the advantages that the nutritional requirements of catfish and water environments are combined with the ingestion features of the catfish to optimize a raw material formula, raw materials are scientifically compounded to greatly increase palatability and feed utilization rate, and the attractant comprises compound flavor peptide which has high anti-oxidation effect and is capable of removing free radicals; the high-yield catfish feed attractant contains saccharomyces cerevisiae culture with functional features such as high nutrition, high bioactivity and high oxidation resistance, Prunus Humilis Bunge extract which can effectively supplement calcium, iron and soluble fibers, has a powerful embedding effect and can prolong shelf life, wormwood seed powder which can evidently increase an anti-freezing effect and stability, increase water resistance and glossiness, enhance and promote palatability and lower powder content, and wheat malt powder which can provide rich nutritional substances, bioactive substances, fragrant substances and plant enzyme sources; the prepared high-yield catfish feed attractant is high in palpability and good in production performance.

The invention discloses a sprouted whole grain with strong inoxidizability and a preparation method for the sprouted whole grain, and belongs to the technical field of grain deep processing. Ultrasonic cleaning is conducted in the soaking process, a high voltage pulse electric field, biological enzymolysis, or combination of the high voltage pulse electric field and the biological enzymolysis is adopted for treatment, a segmented soaking and sprouting manner is adopted for sprouting, the sensory quality, the sprouting yield and quality of the sprouted whole grain are improved, and the sprouting loss is reduced. The preparation method is simple, the soaking and sprouting temperature is low, the period is short, the energy is saved, environment can be protected, the content of functional, nutritional and bioactive materials in the sprouted whole grain is obviously increased, and the food safety is obviously improved. Husked rice can be taken as an example, and according to the sprouted husked rice prepared by the method, the content of gamma-aminobutyric acid is 303.8-312.6 mg / 100 g, the content of glutathione is 16.8-18.3 mg / 100g, the content of inositol hexaphosphate (IP6) is 460.8-484.4 mg / 100 g, and the content of dietary fiber is 3.5-3.6 mg / 100g.

The invention discloses a germinant whole grain with high bioactivity and a preparation method thereof. Ultrasonic cleaning is performed on the whole grain during the soaking process, high-voltage pulsed electric field treatment or biological enzymolysis, or both high-voltage pulsed electric field treatment and biological enzymolysis is / are performed, and the sectionalized soaking and germinant manner is adopted for germination, so that the sensory quality, germinant yield and quality of a germinant whole grain product are improved, and the germinant loss is reduced. The preparation method is simple, the soaking and germinant temperature is low, the cycle is short, energy is saved, and environmental friendliness is realized. The functionality, trophism, content of a bioactivity substance, and food safety of the germinant whole grain product are remarkably improved. Taking brown rice as an example, 100 g of the germinant brown rice prepared through the preparation method disclosed by the invention contains 303.8-312.6 mg of gamma-aminobutyric acid, 16.8-18.3 mg of glutathione, 460.8-484.4 mg of IP6 and 3.5-3.6 mg of dietary fiber.

The invention discloses Argentine cream pumpkin powder. The Argentine cream pumpkin which is rich in microelement cobalt and pectin is taken as a raw material, by-products, including pumpkin seeds, pumpkin leaves and pumpkin bee pollen, in deep processing of the pumpkin are fully used as raw materials, a low-temperature processing technique is adopted in the whole process, and any essences, spices, food additives or chemical substances are not added, so that natural flavor, quality and taste of the pumpkin are extremely maintained, and the food safety and extraction efficiency of the pumpkin powder ingredient materials are effectively promoted; various functional extracts are scientifically compounded and synergistically act, so that the nutrition, functionality, instant dissolving property, liquidity, healthcare effect and guarantee period of the pumpkin powder are obviously enhanced; a new efficient method is developed for pumpkin planting and deep processing; the economic income of farmer households is increased; the problem of environmental pollution caused by abandoned by-products is solved; the issues of agriculture are effectively solved; and the Argentine cream pumpkin powder has certain economic and social values.

The invention relates to the technical field of microbial denitrogenation, in particular to a denitrification microbe enrichment method. The enrichment method comprises the steps that an inoculation source is ground under an anaerobic condition, screened and washed with an anaerobic culture medium, and inocula are obtained; the inocula are subjected to enrichment culture, wherein the concentrationof the inocula is 1-3 g VSS / L, and the initial concentrations of NO3<->-N, NO2<->-N and NH4<+>-N are 30-80 mg / L, 5-10 mg / L and 30-80 mg / L respectively. According to the method, methane is used as anelectron donor, no carbon source needs to be additionally added, aeration for oxygen supplementation is not needed, the cost is saved, the excess sludge output is reduced, and the greenhouse effect isretarded. The nitrification-denitrification denitrogenation process is simplified, and multiple nitrogen sources are removed. The microbe enrichment time is obviously shortened, the enrichment time is only one twelfth to one quarter of the original time, the denitrogenation speed is obviously increased, and the speed is increased by 5-10 times.

The present invention provides a method for enriching nitrite nitrogen oxidizing bacteria granule sludge through process control. According to the method, a SBR apparatus is mainly used, influent nitrite nitrogen concentration (150-2000 mg NO2<->-N / L) is gradually increased, a precipitation time is adopted as a selection element (a precipitation time is 1-10 min), and a DO-MSC (dissolved oxygen-mobile slope change) process control method is adopted to enrich nitrite nitrogen oxidizing bacteria granule sludge. According to the present invention, the DO-MSC process control is applied in the enrichment process of nitrite nitrogen oxidizing bacteria granule sludge so as to save a reaction time, save aeration, and achieve rapid enrichment of nitrite nitrogen oxidizing bacteria granule sludge, wherein the enriched sludge concentration can be more than 10 g / L, the amount of NOB is more than 80% of the total amount of bacterial, and waste water with an influent concentration of 2000 mg NO2<->-N / L can be completely treated.

The invention discloses a high-digestion high-utilization-rate catfish feed, and belongs to the technical field of processing of catfish feeds. Based on nutritional requirements of catfish and water body environment, the digestion characteristics of the catfish are combined, the formula of a conventional catfish feed is optimized, and a feed complex enzyme, wormwood seed powder, a prunus humilis bunge extract and a phagostimulant are scientifically compounded, so that the catfish feed can be prepared, wherein the feed complex enzyme can effectively degrade anti-nutritional factors in the catfish feed, increase the utilization rate of nutrient substances of starch, protein, minerals and the like, increase the protein level, the organism antioxidative activity and the digestive absorption rate of the catfish, significantly increase the digestibility and the production properties of the catfish, reduce the feed coefficient, prevent the water bodies from being polluted and maintain good water quality is scientifically compounded; the wormwood seed powder can significantly improve the caking property, the water resistance, the glossiness and the frost resistance of the catfish feed and reduce the powder containing rate of the feed; the prunus humilis bunge extract can effectively complement calcium, ferrum and soluble fibers; the phagostimulant can substantially improve the palatability of the feed; and therefore, the catfish feed is good in feed coefficient, high in digestibility, good in production properties, high in propagation properties, high in disease resistance, high in palatability and low in cost.

The invention discloses a method for diluting an aluminum grain deoxidizing agent. The method comprises the following steps: before aluminum-contained steel enters an LF furnace for refining, aluminumgrains and a carburant are uniformly mixed according to a mass ratio of 2-3: 1; and after molten steel enters a heating station, the aluminum grain and carburant mixture of 0.75-1.0 kg / t steel is fedin the center position of a ladle along an electrode descending inlet. The aluminum grains are mixed with the carburant, so that the number of the aluminum grains in unit volume is reduced, the combustion loss of the aluminum grains on the surface of slag is reduced, the absorption rate of the aluminum grains is increased, and the reduction of the production cost is achieved.

The invention discloses a grape composite powder and a preparation method thereof. The grape composite powder is prepared from grape powder with natural color and flavor, lactobacillus plantarum powder, wheat malt powder, modified dietary fiber, enzyme powder, papaya powder and other functional products through scientific compounding, the nutrition, the food safety and the health care function of the grape composite powder are improved, and the probiotic property and the quality stability of the grape composite powder are remarkably improved. Compared with existing grape composite powder, the grape composite powder contains simple components and has natural color, flavor and taste, high nutritional value and a good healthcare function, the content of bioactive substance is high, the number of viable bacteria of probiotics is high, the probiotic property is good, the functional metabolin variety of probiotics is complete, the content is high, the food safety is high, and the guarantee period is long.

The invention discloses an anaerobic ammonium oxidation bacterium culture device. The anaerobic ammonium oxidation bacterium culture device comprises a biological culture reactor and a heat preservation device for heat preservation of the biological culture reactor. The biological culture reactor comprises a reactor body and nonwoven fabric filler; the reactor body is a columnar cavity with two ends sealed by flanges, the bottom flange of the reactor body is connected with a water inlet, the top flange of the reactor body is connected with an exhaust port, and the upper portion of the reactor body is provided with a water outlet; the nonwoven fabric filler is filled in the reactor body. Compared with the prior art, the anaerobic ammonium oxidation bacterium culture device has advantages that the nonwoven fabric filler high in biocompatibility is suspended in the reactor and large in water contact area due to bunchy arrangement, anaerobic ammonium oxidation bacteria are prone to adhesion to the filler and less prone to loss along with water, and accordingly bacterium enrichment time is greatly shortened, the concentration of precipitated suspended solid is reduced, and slurry generation quantity is effectively decreased.

The invention provides a device and method for rapidly enriching complete ammonia oxidizing bacteria from activated sludge and application, and belongs to the technical field of feed water treatment. The device mainly comprises a main reactor, a water inlet bucket, a waste liquid bucket, a water inlet peristaltic pump, a water outlet peristaltic pump, a water inlet pipe, a water outlet pipe, a stirring motor, polyurethane filler, Kaldness3 and a membrane assembly. According to the method, the activated sludge is cultured in a continuous water inlet and outlet mode, ammonium chloride is used as the only ammonia nitrogen source, water is fed under the ammonia nitrogen oligotrophic condition, the optimal culture medium and filler are used in a matched mode, the q-PCR detection means is adopted, and finally the highest proportion of complete ammonia-oxidizing bacteria in the Copy number of all ammonia-oxidizing microorganism genes is obtained. The beneficial effect of quickly enriching the complete ammonia oxidizing bacteria from the activated sludge is achieved. According to the method, the enrichment time of the complete ammonia oxidizing bacteria is greatly shortened, the enrichment effect of the complete ammonia oxidizing bacteria is comprehensively improved, and the method has the advantages of accuracy, high efficiency, rapidness and the like.

The present invention discloses probiotics containing grape compound powder and a preparation method thereof. The grape powder with natural color and luster, flavor and taste are scientifically matched with functional products of lactobacillus plantarum powder, wheat malt powder, modified dietary fibers, enzyme powder, papaya powder, etc. The preparation method effectively improves the nutrition, food safety and health-care function of the grape compound powder and significantly improves the probiotic property and quality stability of the grape compound powder. Compared with the existing grape compound powder, the probiotics containing grape compound powder is simple in components, natural in color and luster, flavor and taste, high in nutritional value, strong in health-care function, high in contents of bioactive substances, high in number of viable probiotics, strong in probiotic properties, full in types and high in contents of probiotic functional metabolites, high in food safety, and long in shelf life.

The invention discloses compound protein powder long in storage life and a production method thereof. The production method is characterized in that isolated soybean protein, concentrated whey protein and phospholipid are used as main raw materials; stevia rebaudiana is subjected to low-temperature processing to obtain stevia rebaudiana extract, so that the effective components and the bioactive components of the stevia rebaudiana can be kept to the maximum extent, high extraction rate is achieved, and the stevia rebaudiana extract is pure in flavor, natural in color and free of radix glycyrrhizae flavor; the stevia rebaudiana extract is scientifically compounded with the main raw materials and anti-freeze peptides, dietary fiber powder, honey bee pupae insect enzymatic hydrolysate and wheat malt powder are added to produce the enzymatic hydrolysate which is appropriate in taste, good in solubility, comprehensive in nutrition, high in bioactivity, widely applicable to people and long in storage life. The production method has the advantages that the non-thermal processing technology is used, and the method is high in working efficiency, low in energy consumption, environmentally friendly, free of pollution, capable of reducing the introduction of food additives and chemical auxiliaries, capable of effectively increasing food safety, capable of increasing product quality and lowering production cost, capable of achieving a low-carbon production goal and capable of achieving large-scale and industrial development.

The invention discloses a device and a method for converting ammonia nitrogen in domestic sewage into N2O. The device comprises a hollow fiber membrane biofilm reactor which microorganisms are attached to and grow on, and the biofilm reactor is respectively communicated with a water inlet pipe, an N2O collecting pipeline and an integrated activated sludge reactor; the integrated activated sludge reactor is provided with a microporous aerator and an inclined plate, and part of effluent is communicated with a return pipe to the biofilm reactor; and N2O generated in the biofilm reactor is separated and collected in real time through the negative pressure of the inner cavity of the hollow fiber membrane. The separation efficiency of the low-concentration N2O is improved, the enrichment time ofthe N2O is shortened, and meanwhile, liquid in the reactor is prevented from entering the inner cavity. According to the invention, low-concentration ammonia nitrogen in domestic sewage can be converted into N2O, and the conversion rate is higher than 85%.

The invention discloses nutritive compound protein powder and a preparation method thereof. Soybean protein isolate, a whey protein concentrate and phosphatide are used as main raw materials of the nutritive compound protein powder. Stevia rebaudiana is processed into a stevia rebaudiana extract at low temperature, so that effective components and bioactive components in the stevia rebaudiana can be reserved to the maximum extent, the extraction rate is high, the flavor is pure, the color is natural, and taste of licorice roots does not exist; and the stevia rebaudiana extract is scientifically compounded with the main materials, and antifreeze polypeptide, dietary fiber powder, a bee pupa enzymatic hydrolysate and wheat malt flour are added, so that the compound protein powder which is appropriate in mouth feel, good in solubility, comprehensive in nutrients, high in bioactivity and suitable for broad crowds is prepared. According to the preparation method, a non-thermal processing technique is adopted, so that the working efficiency is high, the energy consumption is low, and the nutritive compound protein powder is environmentally friendly and pollution-free; the introduction quantity of food additives and the introduction quantity of chemical addition agents are reduced, so that the food safety is effectively improved, the product quality is improved, and the production cost is reduced; and therefore, the low-carbon production target is realized, and scale and industrialized development can be realized.

The present invention relates to a method for depletion of undesired molecules and / or enrichment of desired molecules from a sample comprising high abundant as well as low abundant molecules, comprising the following steps: a) providing a separation material comprising a solid phase (beads) comprising an inner porous core material comprising magnetic particles and an outer porous shell with a porosity equal or denser than that of the shell; b) adding the sample to the separation material; c) adsorbing a first fraction of molecules with a molecular weight of 500-50000 Da in the core and simultaneously excluding a second fraction of molecules from binding to the core and the shell, wherein the molecular weight of the second fraction molecules is at least 5 preferably 10 times higher than the molecular weight of the first fraction and d) eluting the desired molecules from the separation material, wherein step d) and optionally step c)is performed using an oscillating power / field applied over the separation material. The first fraction of molecules are for example drugs with a mw of about 700 Da, small proteins / peptides with an mw of about 7000 Da or proteins with a mw of about 40000 Da.

The invention discloses a radioactive nuclide enrichment method and device, and a system with same. The method comprises steps of collecting to-be-tested liquid, and controlling flow of the to-be-tested liquid; in the process of concentration and enrichment, heating and stirring to evaporate the to-be-tested liquid according to a preset target temperature difference and a target boiling heat transfer requirement, so as to enable evaporation speed to meet preset speed; after cleaning, collecting concentrated solution after enrichment of the radioactive nuclide, and obtaining a radioactive nuclide enrichment result of the to-be-tested liquid according to the concentrated solution. In the prior art, enrichment takes long time and is slow in speed, enrichment relies on technical level of an operator, and data of the same batch of processing is less consistent. The method solves the above technical problems. The vacuum, low-temperature enrichment method shortens enrichment time greatly, enables automatic operation, makes enriched samples more consistent, and is easy to implement.

The invention relates to the field of molecular biology, and in particular relates to a circulating tumor cell negative enrichment method. The method comprises the following steps that pretreatment is carried out on a blood sample by adopting density gradient centrifugation on the basis of a lymphocyte separating tube, so that the operating step of separating leukocytes is simplified, the enrichment time of the circulating tumor cell is shortened, and the whole operation is competed within 2.5h; the number of the left leukocytes is 30-50 % of that of original leukocytes of the blood sample, self-made negative enrichment immunomagnetic beads CD45 are adopted for marking the leukocytes and removed, so that the used number of the immunomagnetic beads is reduced, and the cost for negative enrichment of the circulating tumor cells is greatly saved. The lymphocyte separating tube is adopted for carrying out pretreatment on the blood sample, the negative enrichment method of the circulating tumor cell is improved, the stability of the enrichment operation is effectively improved, and the recovery ratio of the circulating tumor cell achieves 85% or above. The circulating tumor cell negative enrichment method further has the advantage of high sensitivity, and the circulating tumor cells can be stably detected within a few samples.

The invention discloses an Argentine butternut squash health-care food. The Argentine butternut squash health-care food takes byproducts, namely squash seeds and squash leaves, in a deep processing process of squashes as raw materials; a whole process adopts a low-temperature processing technology, and any essence, spice, food additive and chemical substance are not added; the natural flavor, quality and mouth feel of the squashes are kept to the greatest extent; the food safety and the extraction rate of squash powder component raw materials are effectively improved; a plurality of types of functional raw materials including squash fermented powder, polyglucan, squash malt powder, aloe gel lyophilized powder, squash leaf powder, winter rye peptide, resistant dextrin, beta-glucan and oligosaccharide are scientifically compounded and have synergistic effect so that the nutrition property, functionality, instant solubility, flowability, health-care effect and shelf life of squash powder are remarkably enhanced. Meanwhile, a new way is explored for planting and deep processing of the squashes; the economic income of farmers is increased; environment pollution caused by the fact that the byproducts are wasted is solved, three problems about farmers, rural society and agriculture are effectively solved, and the Argentine butternut squash health-care food has certain economic and social values.