Method for enriching vitamin K2 in natto bacillus fermentation liquid
A technology of Bacillus natto and fermented liquid, which is applied in the field of bioengineering and bioseparation engineering, can solve the problems of high difficulty in purifying vitamin K, complicated overall process, and high cost, and achieves cheap and easy-to-obtain raw materials, fast separation rate, The effect of reducing dosage
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Embodiment 1
[0033] Embodiment 1: Vitamin K in a kind of enrichment Bacillus natto fermented liquid 2 Methods
[0034] (1) Obtaining the supernatant of the fermentation broth
[0035] The fermented liquid processed in this embodiment is 69.6g / L by glycerol, glucose is 34.5g / L, K 2 HPO 4 4.0g / L peptone, 20g / L peptone, 25g / L yeast extract, 250rpm, cultured at 37°C for five days to obtain Bacillus natto fermentation broth.
[0036]The above fermented liquid of Bacillus natto was centrifuged and filtered to remove bacteria and sediment to obtain a supernatant.
[0037] (2) Flocculant purification of fermentation broth
[0038] First add acetic acid to water to form a 0.18%wt acetic acid solution, then add chitosan to the 0.18%wt acetic acid solution to form a 0.4%wt chitosan acetic acid solution.
[0039] Take 100m of the above-mentioned supernatant from the removal of bacteria, add 9g of the above-mentioned chitosan acetic acid solution to it, stir for 30min, finally add filter aid, disc...
Embodiment 2
[0052] Embodiment 2 A kind of vitamin K enriched in Bacillus natto fermented liquid 2 Methods
[0053] A kind of vitamin K enriched in Bacillus natto fermented liquid 2 method, including the following steps:
[0054] Step 1: Centrifuge the fermented liquid of Bacillus natto, and collect the supernatant;
[0055] Step 2: Add flocculant and filter aid to the supernatant obtained in step 1, then stir, then centrifuge, and collect the supernatant;
[0056] Step 3: Add reduced Fe to the supernatant from Step 2 3 o 4 @SiO 2 -G nano-microspheres, adjust the pH value to 6.0, then carry out stirring treatment at 20°C for 10minmin, and then collect the magnetic material after adsorption; the reduced Fe 3 o 4 @SiO 2 -The preparation method of G nano microspheres comprises the following steps:
[0057] S1, the Fe 3 o 4 The magnetic microspheres were added to the mixed solution composed of deionized water, isopropanol ammonia water and tetraethyl orthosilicate, stirred evenly an...
Embodiment 3
[0067] Embodiment 3: Vitamin K in a kind of enrichment Bacillus natto fermented liquid 2 Methods
[0068] A kind of vitamin K enriched in Bacillus natto fermented liquid 2 method, including the following steps:
[0069] Step 1: Centrifuge the fermented liquid of Bacillus natto, and collect the supernatant;
[0070] Step 2: Add flocculant and filter aid to the supernatant obtained in step 1, then stir, then centrifuge, and collect the supernatant;
[0071] Step 3: Add reduced Fe to the supernatant from Step 2 3 o 4 @SiO 2 -G nano-microspheres, adjust the pH value to 8.0, then carry out stirring treatment at 35°C for 30min, and then collect the magnetic material after adsorption; the reduced Fe 3 o 4 @SiO 2 -The preparation method of G nano microspheres comprises the following steps:
[0072] S1, the Fe 3 o 4 The magnetic microspheres were added to a mixed solution composed of deionized water, isopropanol ammonia and tetraethyl orthosilicate, stirred evenly and reacte...
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