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Denitrification microbe enrichment method

A microbial and enrichment technology, applied in the field of microbial denitrification, can solve the problems of prolonged microbial enrichment time, small mass transfer coefficient, and long enrichment time, so as to reduce excess sludge production, promote research and application, and shorten enrichment set time effect

Inactive Publication Date: 2018-09-21
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since DAMO microorganisms are autotrophic microorganisms, they grow slowly and their doubling time is several weeks or even months, so the problems of long enrichment time and low activity have always been the main factors that limit the research of DAMO microorganisms and the development and application of related technologies.
[0004] At present, the biological denitrification technology using methane as an electron donor is still in the theoretical research stage, and the main factors that limit its practical application are the very long enrichment time of microorganisms and the limited denitrification capacity.
Due to the low solubility of methane in the water phase and the small mass transfer coefficient, the mass transfer process of methane from the gas phase to the liquid phase and then to the microorganisms is greatly restricted, resulting in the prolongation of the enrichment time of microorganisms, and the continuous aeration causes CH 4 Leakage and waste, there are also potential safety hazards
Therefore, the existing DAMO microorganism culture method is not conducive to industrial application
Generally, microbial enrichment takes 4.5 months to more than 1 year, and can only remove NO 3 - -N or NO 2 - -N, the denitrification rate is usually 10-20mg / L / d, which cannot meet the needs of practical applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 NO 3 - , NO 2 - , NH 4 + and CH 4 Enrichment of denitrification microorganisms under methane as electron donor conditions

[0053] Select freshwater river sediment at a depth of 0.2-0.4m as the inoculation source, grind it in an anaerobic glove box, pass through a 280 μm sieve to remove bulky impurities, and then wash and centrifuge with deaerated anaerobic mineral salt medium for 3 Second, the dissolved organic matter is removed to obtain the inoculum.

[0054] Adjust the inoculum concentration to 1g VSS / L, NO 3 - -N concentration is 50mg / L, NO 2 - -N concentration is 5mg / L, NH 4 + -N concentration is 50mg / L, pH is 7.5, temperature is 35°C, headspace gas is CH 4 and CO 2 (95 / 5), the pressure is 1.1atm. In a continuous stirred tank reactor, the enrichment culture is carried out with anaerobic mineral salt medium.

[0055] The formula of the mineral salt medium used is: every liter of medium contains KHCO 3 0.5g, KH 2 PO 4 0.05g, MgSO 4 ·7...

Embodiment 2

[0058] Example 2 NO 3 - , NO 2 - , NH 4 + and CH 4 Enrichment of denitrification microorganisms under methane as electron donor conditions

[0059] Select freshwater river sediment at a depth of 0.2-0.4m as the inoculation source, grind it in an anaerobic glove box, pass through a 280 μm sieve to remove bulky impurities, and then wash and centrifuge with deaerated anaerobic mineral salt medium for 3 Second, the dissolved organic matter is removed to obtain the inoculum.

[0060] Adjust the inoculum concentration to 2g VSS / L, NO 3 - -N concentration is 30mg / L, NO 2 - -N concentration is 10mg / L, NH 4 + -N concentration is 30mg / L, pH is 7.0, temperature is 36°C, headspace gas is CH 4 and CO 2 (90 / 10), the pressure is 1.2atm. In a continuous stirred tank reactor, the enrichment culture is carried out with anaerobic mineral salt medium.

[0061] The formula of the mineral salt medium used is: every liter of medium contains KHCO 3 0.5g, KH 2 PO 4 0.05g, MgSO 4 ...

Embodiment 3

[0064] Example 3 NO 3 - , NO 2 - , NH 4 + and CH 4 Enrichment of denitrification microorganisms under methane as electron donor conditions

[0065] Select freshwater river sediment with a depth of 0.2-0.4m as the inoculation source, grind it in an anaerobic glove box, pass through a 280 μm sieve to remove bulky impurities, and then wash and centrifuge with deaerated anaerobic mineral salt medium for 3 Second, the dissolved organic matter is removed to obtain the inoculum.

[0066] Adjust the inoculum concentration to 3g VSS / L, NO 3 - -N concentration is 80mg / L, NO 2 - -N concentration is 8mg / L, NH 4 + -N concentration is 80mg / L, pH is 8.0, temperature is 34°C, headspace gas is CH 4 , the pressure is 1.0atm. In a continuous stirred tank reactor, the enrichment culture is carried out with anaerobic mineral salt medium.

[0067] The formula of the mineral salt medium used is: every liter of medium contains KHCO 3 0.5g, KH 2 PO 4 0.05g, MgSO 4 ·7H 2 O 0.2g, C...

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Abstract

The invention relates to the technical field of microbial denitrogenation, in particular to a denitrification microbe enrichment method. The enrichment method comprises the steps that an inoculation source is ground under an anaerobic condition, screened and washed with an anaerobic culture medium, and inocula are obtained; the inocula are subjected to enrichment culture, wherein the concentrationof the inocula is 1-3 g VSS / L, and the initial concentrations of NO3<->-N, NO2<->-N and NH4<+>-N are 30-80 mg / L, 5-10 mg / L and 30-80 mg / L respectively. According to the method, methane is used as anelectron donor, no carbon source needs to be additionally added, aeration for oxygen supplementation is not needed, the cost is saved, the excess sludge output is reduced, and the greenhouse effect isretarded. The nitrification-denitrification denitrogenation process is simplified, and multiple nitrogen sources are removed. The microbe enrichment time is obviously shortened, the enrichment time is only one twelfth to one quarter of the original time, the denitrogenation speed is obviously increased, and the speed is increased by 5-10 times.

Description

technical field [0001] The invention relates to the technical field of microbial denitrification, in particular to a method for enriching denitrification microorganisms. Background technique [0002] Methane (CH 4 ) is an energy source and a strong greenhouse gas, which can be produced in sewage treatment plants, and CH in the gas phase 4 Easy recovery and utilization, but CH dissolved in the liquid phase 4 Recycling is difficult, and slow release into the air will increase the greenhouse effect. The commonly used denitrification process in sewage treatment plants is the activated sludge method. The denitrification is achieved through the nitrification-denitrification process. Sometimes additional carbon sources are required to achieve the ideal denitrification effect. Additional carbon sources will lead to increased excess sludge and microbial metabolism. Product increase, cost increase and other issues. Therefore, using CH 4 Nitrogen removal technology as a carbon sou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02
CPCC12N1/02
Inventor 曾建雄付亮陆勇泽丁静丁兆威
Owner UNIV OF SCI & TECH OF CHINA
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