The invention relates to an online low-pressure, middle-pressure and high-pressure combined two-dimension preparation liquid chromatographic
system. Two one-dimension low-pressure, middle-pressure and high-pressure preparation liquid chromatographic systems are organically combined through a six-way valve and
software. Pressure used by a second dimension
system is not lower than pressure used by a first dimension
separation system. When the first dimension
system and the second dimension system are low-pressure or middle-pressure preparation liquid chromatographic systems, through switching the six-way valve, target compound directly enters a second dimension
chromatographic column for second dimension separation after first dimension separation; and when pressure used by a second dimension
separation system is higher than pressure used by the first dimension
separation system, a
trapping column is connected on the six-way valve, the target compound is adsorbed on the head of the
trapping column after the first dimension separation, and then the target compound enters the second dimension
chromatographic column for second dimension separation. The invention has the advantages that the contradiction between sample
injection volume and separation capacity is solved, the
large sample injection volume is ensured and the separation capacity of the preparation chromatographic system is greatly improved; the
system structure is simple and the establishment is easy; the operation is simple and convenient and the
automation can be easily realized; and the application scope is wide.