53results about How to "Reduce cell damage" patented technology

The invention provides a novel method of dissociating anchorage independent and dependent cell aggregates. The invention also includes the cells resulting from the methods of the invention and the use of the cells in various applications requiring the generation of a single cell suspension.

Sperm, oocyte, and embryo survival and function is improved in vivo or in vitro by the use of a polysaccharide containing arabinose, galactose and/or hexuronic acid. In particular, a nonspermicidal lubricant containing such a polysaccharide (e.g., gum arabic, pectin, or galacturonic acid) increases the fertilization potential of the sperm during coitus, artificial insemination or sperm collection. Similarly, a freezing medium containing a polysaccharide containing arabinose, galactose and/or hexuronic acid enhances sperm, oocyte, or embryo viability.

Provided herein are compounds of Formula I, compositions comprising an effective amount of a compound of Formula I, and methods for preventing, reducing or treating retinal ganglion cell damage comprising administering an effective amount of a purine derivative to a subject in need thereof.

The present invention relates to a one-photon and/or two-photon fluorescent probe for selectively detecting hydrogen sulfide in the human body using a compound including an α,β-unsaturated carbonyl group and an acedan (2-acyl-6-dimethyl-amino-naphthalene) fluorescent material; to an imaging method of hydrogen sulfide in cells using the same; and to a manufacturing method of the fluorescent probe. More specifically, in the fluorescent probe of the present invention, the α,β-unsaturated carbonyl group of the compound selectively binds to hydrogen sulfide, inducing an increase in fluorescence of the acedan fluorescent material. The fluorescent probe according to the present invention can be conveniently synthesized, enables two-photon excitation, and corresponds to a small-molecule probe having stability and low toxicity in the body. In addition, the fluorescent probe according to the present invention can exhibit a fluorescent change by selectively reacting with hydrogen sulfide, thereby imaging the distribution of hydrogen sulfide in cells or tissues, and thus can be useful for a composition for imaging and an imaging method.

The present invention relates to cryopreserved cell cultures, methods for cryopreserving cells and methods for conducting cellular assays on such cells. A cryopreserved cell culture of the invention comprises a container having at least a surface which is coated with poly-lysine and frozen cells supported on the surface.

The present invention relates to a method for preparing a garlic-fermented composition and a garlic-fermented composition prepared by the method, and the purpose of the present invention is to provide a garlic-fermented composition which is easy to take, has no side effect, and improves excellent pharmacological and physiological efficacies of garlic. To achieve this, the method for preparing a garlic-fermented composition according to an aspect of the present invention, comprises: a first fermentation step of mixing garlic, water, and a ferment strain, followed by fermentation, thereby decomposing and converting sugars and proteins of the garlic; and a second fermentation step of additionally supplying a carbon source to a filtrate obtained through solid-liquid separation of the fermented product, followed by fermentation, thereby removing ammonia of the filtrate and decomposing and converting the additionally supplied carbon source.

A device for cardiocirculatory assistance constituting a pump for a blood flow includes a body, a pair of covers, and a pair of membranes. The device is provided with a circuit for the passage of a gas or gaseous fluid alternatively under pressure and depressurization so that a reciprocating pumping motion of the membranes is established.

The present invention provides a method for cell rejuvenation, including contacting cells with a composition containing a Rosa roxburghii fruit extract obtained by extraction of a Rosa roxburghii fruit using water, alcohol, or mixtures of water and alcohol as solvents. Said extract improves DNA repair, reduces cell damage caused by oxidative stress or inflammation, maintains the normal functions of genes, proteins, and mitochondria, facilitates telomerase reaction and reduces telomere damage by chemicals or UV radiation, thereby improving cell rejuvenation.