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Culture medium for separating actinomycetes in high-salt environment and application thereof

A technology of culture medium and actinomycetes, which is applied in the direction of isolating microorganisms and bacteria, etc., can solve the problems that there are no reports on the culture medium of various actinomycetes and no breakthrough in pure culture technology

Inactive Publication Date: 2013-11-06
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, the isolation method has always been the "bottleneck" that restricts people's development and utilization of this kind of microbial resources, especially the pure culture technology of actinomycetes in high-salt environment has not been broken, especially for actinomycetes in high-salt environment, there is no report A culture medium suitable for various actinomycetes

Method used

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  • Culture medium for separating actinomycetes in high-salt environment and application thereof
  • Culture medium for separating actinomycetes in high-salt environment and application thereof
  • Culture medium for separating actinomycetes in high-salt environment and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment one: the preparation of the culture medium that separates high-salt environment actinomycetes

[0018] In terms of weight percentage, the culture medium consists of 1% mannitol, 0.1% alanine, K 2 HPO 4 0.1%, MgSO 4 ·7H 2 O 0.1%, CaCO 3 0.02%, FeSO 4 ·7H 2 O 0.001%, agar 1.8%, trace salt 0.0003%, multivitamin 0.0004%, biotin 0.000025%, potassium dichromate 0.005%; among them, trace salt is composed of FeSO 4 ·7H 2 O, MnCl 2 4H 2 O and ZnSO 4 ·7H 2 O is composed of 0.0001% each; multivitamins are composed of B1, B2, B6, niacin, inositol, pantothenic acid, p-aminobenzoic acid, and folic acid each of 0.00005%; pH 7.2-7.4.

Embodiment 2

[0019] Embodiment two: the soil sample for testing of the culture medium of separating high-salt environment actinomycetes

[0020] The salt lake samples were collected from Nierkule Lake in Artush City, Xinjiang. The lake is distributed in a northeast-southwest direction, with a length of 18-20 km, a width of 2-4 km, and an area of ​​45 km 2 , the altitude is 1585 m ( 76°53' E, 40°10' N ). Immediately after collection, the samples were packed into sterile ziplock bags and placed in the car refrigerator. Store at -20°C after delivery to the laboratory for future use. One of the sediment samples was selected in the experiment to study the separation model.

[0021] 1. Determination of sample ion composition

[0022] Mainly measure CO in the sample 3 2- , Cl - , SO 4 2- , Ca 2+ , Mg 2+ 、Na + 、K + The content and total soluble salt content were completed by Xinjiang Production and Construction Corps Tarim Basin Biological Resources Conservation and Utilization Key ...

Embodiment 3

[0028] Embodiment three: the influence of classical culture medium on actinomycetes isolation

[0029] Choose 6 kinds of classical substratum better of the isolated actinomycetes that have been reported in the prior art, classic substratum can represent the common representative substratum of actinomycetes field, the result is shown in Table 2 as can be seen, obtains 6 altogether A genus of 16 different species of actinomycetes. TP medium and F6 medium had the best separation effect, both of which could obtain 3 genera and 5 different species of actinomycetes. GW1 medium, HV medium and SCK medium have the second best separation effect. GW1 medium can obtain 3 genera and 4 different species of actinomycetes, and HV medium and SCK medium can obtain 3 genera and 3 different species. of actinomycetes. From the perspective of medium composition, the carbon source used in TP medium is trehalose, the nitrogen source is proline; the carbon source of F6 medium is chitosan, the nitro...

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Abstract

The invention discloses a culture medium for separating actinomycetes in a high-salt environment and application thereof. The culture medium is composed of 1% of mannitol, 0.1% of alanine, 0.1% of K2HPO4, 0.1% of MgSO4.7H2O, 0.02% of CaCO3, 0.001% of FeSO4.7H2O, 1.8% of Agar, 0.0003% of trace salt, 0.0004% of compound vitamin, 0.000025% of biotin and 0.005% of potassium dichromate, wherein the trace salt is composed of 0.0001% of FeSO4.7H2O, 0.0001% of MnCl2.4H2O and 0.0001% of ZnSO4.7H2O; and the compound vitamin is composed of 0.00005% of vitamin B1, 0.00005% of vitamin B2, 0.00005% of vitamin B6, 0.00005% of nicotinic acid, 0.00005% of inositol, 0.00005% of pantothenic acid, 0.00005% of paraaminobenzoic acid and 0.00005% of folic acid. The culture medium is suitable for separating actinomycetes in an oligotrophic high-salt environment which has a high salt content, and has wide application values.

Description

technical field [0001] The invention relates to the technical field of industrial microorganisms, in particular, the invention specifically relates to a culture medium for isolating actinomycetes in a high-salt environment and the technical field of application thereof. Background technique [0002] According to the prior art, the pure culture of microorganisms obtained from environmental samples by general isolation methods has a very high reproducibility, and it is difficult to isolate new species. Especially actinomycetes, because they produce a variety of biologically active secondary metabolites, they have attracted widespread attention from microbiologists. However, most of the strains screened by common isolation methods are known and common bacteria. This brings great difficulty to the screening of current active substances. Therefore, it has become a long-term research task and difficulty in the field of microbiology to explore effective microbial isolation methods...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02
Inventor 张利莉陈正军李艳宾关统伟夏占峰张瑶张娇吕玲玲贾晓宇
Owner TARIM UNIV
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