The invention relates to a method for industrially producing porcine reproductive and respiratory syndrome (PRRS) vaccines by utilizing a
bioreactor. The method comprises the following steps: (1) sterilizing a
microcarrier and the
bioreactor, then adding
cell growth liquid, inoculating cells for vaccine preparation for culturing, inoculating PRRS viruses when dense monolayers are formed on the cells on the
microcarrier and continuing culturing to reproduce the viruses; (2) stopping culturing and harvesting
virus suspension when cytopathy reaches more than 80%; and (3) adding a freeze-
drying protective agent to the harvested
virus suspension to prepare freeze-dried attenuated live vaccines or carrying out ultrafilter concentration as well as
virus inactivation on the harvested
virus suspension, purifying the inactivated viruses by adopting
column chromatography, and adding adjuvants to prepare inactivated vaccines. Compared with the traditional spinner
bottle production process, the method has the following advantages: the
automation control degree is high, so production can be monitored in real time; the labour is saved, thus reducing the cost; the land for production is few, thus being easy to enlarge the scale of production; and the produced viruses have
high titer, so the batch-to-batch variation is small, the product quality is stable and the
side reaction is low.