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Method for industrially producing animal rabies vaccine by utilizing bioreactor

A technology of bioreactor and rabies vaccine, which is applied in the field of production of animal rabies vaccine, achieves the effects of cost reduction, easy expansion of scale, and small batch-to-batch difference

Inactive Publication Date: 2011-05-04
WUHAN CHOPPER BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above documents all relate to the preparation process of human rabies vaccine, but there are no reports on the production process of animal rabies vaccine. The present invention utilizes a stirred bioreactor and adopts a step-by-step enlarged inverted tank type operation technology to carry out cell High-density culture for the preparation of animal rabies vaccine

Method used

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  • Method for industrially producing animal rabies vaccine by utilizing bioreactor
  • Method for industrially producing animal rabies vaccine by utilizing bioreactor
  • Method for industrially producing animal rabies vaccine by utilizing bioreactor

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Embodiment 1

[0023] Bioreactor: 14L and 40L bioreactors from NBS Company of the United States;

[0024] Microcarrier: Cytodex1 (General Electric Healthcare Life Sciences);

[0025] Rabies virus: the adapted virus 10aGV strain of aG strain domesticated by Vero cells;

[0026] Cell growth medium: M199 (Beijing Qingda Tianyi Biotechnology Co., Ltd.) containing 8% calf serum by volume;

[0027] Virus maintenance solution: M199 (Beijing Qingda Tianyi Biotechnology Co., Ltd.) containing 1% calf serum by volume;

[0028] Cell culture: In 14L bioreactors, add Cytodex-1 at a concentration of 10g / L. After hydration, wash with pH7.2 phosphate buffered saline PBS twice, sterilize, add cell growth medium, and inoculate Vero cells were cultured; the parameters of the culture method were: pH 7.2, temperature 37°C, dissolved oxygen 50%, stirring speed 30-100rpm; samples were regularly taken every day to observe the growth of the cells, and the cells were counted to determine the consumption of glucose. ...

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Abstract

The invention provides a method for industrially producing an animal rabies vaccine by utilizing a bioreactor, comprising the following steps of: (1) sterilizing a micro-carrier and the bioreactor, adding a cell growth solution, inoculating Vero cells for culturing, inoculating rabies viruses after the cell on the micro-carrier forms a compact single layer, and continuously culturing to propagate the viruses; (2) inoculating for 18h and then continuously harvesting a virus solution; (3) carrying out ultrafiltration concentration and virus inactivation on the harvested virus solution; and (4) purifying and inactivating the viruses through a column chromatography method to prepare the vaccine. In the invention, the high density culturing of the cells is carried out by utilizing a bioreactor micro-carrier culturing technology to produce the animal rabies vaccine. Compared with the traditional rotating bottle production method, the automation control degree is high, the production can be monitored in real time, the labor power and the cost are reduced, the production land is less, the scale is easy to expand, the produced virus titer is high, the difference among batches is less, the product quality is stable, and the side reaction is less.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to a method for industrially producing animal rabies vaccine using a bioreactor. Background technique [0002] Rabies is a severe zoonotic infectious disease caused by Rabies Virus (RV). About 5,000 to 8,000 people die of rabies in my country every year. In my country, sick dogs are the main source of infection of rabies, and the infection of rabies patients is mainly caused by the bite of rabid dogs (mad dogs and infected dogs), and can also be infected through the digestive tract, respiratory tract mucosa and contact. [0003] my country is a big country in the dog industry, and dogs are getting closer to people. How to prevent and control dog rabies is an important issue in preventing human rabies. [0004] Injecting live rabies vaccines to dogs has a good preventive effect, but it is potentially dangerous. It is of great practical significance to develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/245C12N7/00A61P31/22C12R1/93
Inventor 刘汉平李伟秦红刚温文生漆世华朱薇靖志强廖园园
Owner WUHAN CHOPPER BIOLOGY
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