58results about How to "Realize quantitative determination" patented technology

The invention discloses a magnetic particle-based quantitative chemiluminescent assay kit for an anti-PM-Scl antibody IgG. The kit comprises an anti-PM-Scl antibody IgG calibrator, an anti-PM-Scl antibody IgG quality control product, a Tris buffer containing biotin-labeled PM-Scl antigen and bovine serum albumin, a Tris buffer containing an alkaline phosphatase-labeled goat anti-human polyclonal antibody and bovine serum albumin, a Tris buffer containing streptavidin-labeled magnetic particles and bovine serum albumin, and a rinsing solution. The detection method using the kit improves sensitivity and a linearity range by 3 to 5 orders of magnitudes on the basis of a traditional membrane strip immunization method and a traditional enzyme linked immunosorbent assay method, realizes real quantitative determination, is rapid in reaction and reliable in results, can be automatically used in cooperation with an automatic chemiluminescence immunity analyzer and is of irreplaceable important value to clinical diagnosis.

The invention discloses cysteine hydrochloride in acetaminophen injection and a test method of degradation product cystine. The method adopts efficient liquid chromatography, a flowing phase adopts a phosphate buffer containing ion pairs and an organic solvent, and a gradient evaluation method is adopted. The method can be used for testing the content of cysteine hydrochloride in the acetaminophen injection, controlling the content of the content of cysteine degradation product cystine and testing the content of cysteine hydrochloride raw materials, the cysteine and the cystine have good resolution, the specificity and the sensitivity are high, and the operation is simple and convenient.

The invention discloses a method for determining silicone oil in cosmetics, which comprises the following steps that a test sample is dispersed with water in a color comparison tube; after being dispersed, the test sample is transferred to a separating funnel with water to be fully shaken; silicone oil in the test sample is extracted with normal hexane; after standing, moisture is removed through filtering, and a filtrate is obtained; and then the content of the silicone oil in the filtrate is determined by using the Fourier transform infrared spectroscopy. According to the method, the test sample matrix is dispersed with the water, the silicone oil is fully extracted with the normal hexane, the pretreatment is easy and quick, the dissolving capacity of the normal hexane to the silicone oil is strong, and the self infrared light does not interfere with the quantification of the silicone oil.

The invention discloses a method and device for measuring coal wettability under high pressure through low-field nuclear magnetic resonance. The method comprises the following steps: performing low-field nuclear magnetic resonance measurement on water in pulverized coal under a standard condition to obtain a T2 spectrum of the water in the coal, and according to the position and the amplitude of afirst peak on the right side of the T2 spectrum, acquiring the geometric mean value T2g of the relaxation time T2 of the spectral peak; in combination with a measurement result theta of the water contact wetting angle of the conventional moulded coal surface in a parallel experiment, establishing a relational expression of a water wetting angle by using normalized T2g and NT2g: theta=84.376*NT2g+52.612; according to the relational expression, in combination with the T2g value of the water in the coal under a high-pressure condition, working out the water wetting angle of the coal under a setgas pressure. The device comprises a gas source, a sealed constant-pressure system and a nuclear magnetic magnet, wherein the nuclear magnetic magnet is connected with a low-field nuclear magnetic resonance instrument. According to the method, through establishment of a scaling relation between the coal wettability and a nuclear magnetic resonance T2 spectrum signal, quantitative determination ofthe coal wettability under different gas pressures is achieved; the method is new method for measuring the coal wettability instantly, in situ and dynamically.

The invention discloses a method of measuring isocarbophos by the fluorescent spectrometry of aptamer mediating nitrogen-doped carbon spots catalyzing H2O2 reaction with TMB. The method comprises thefollowing steps that (1), an isocarbophos standard solution system with preset concentration is prepared; (2), a blank comparison solution system is prepared; (3), delta F=F-F0 is calculated; (4), thedelta F is used for conducting a working curve for the concentration of the isocarbophos; (5), a tested sample solution is prepared, and delta F sample=F sample-F0 is calculated; and (6), the isocarbophos concentration in the tested sample is calculated. The catalysis of isocarbophos aptamer reacting mediating nitrogen-doped carbon spots (CD) is adopted, that is, the CD catalyzes the oxidation ofTMB by H2O2 to produce fluorescence products. The CD is combined with the catalysis, and therefore sensitivity is greatly improved. The fluorescence products are used as a fluorescence probe and arein linear relationship with the concentration of the tested isocarbophos. Quantitative measuring of the isocarbophos by nitrogen-doped carbon spots catalytic reacting fluorescent spectrometry is realized. The method is simple and quick, is excellent in selectivity and is high in sensitivity.

The invention discloses a time-varying radiation heat flow experiment system and measurement method based on distance control, and relates to an experiment device and a test system capable of realizing time-varying radiation heat flow (time-varying heat flow). The experiment system comprises a radiation source, a radiation source bearing frame, a radiation source moving bracket, a radiation sourcecontroller, a controller, a driver, a direct-current power supply and an experiment table base. The radiation source moving bracket adopts a guide rail sliding table. A guide rail sliding block is arranged on the guide rail sliding table. A radiation source bearing frame is installed on the guide rail sliding block, and a radiation source is fixedly installed below the radiation source bearing frame. A fixing frame is installed on the lower portion of the guide rail sliding block, a baffle rotating rod is installed on the fixing frame through a bolt, the baffle rotating rod is fixedly connected with a baffle, the baffle is located under the radiation source, and the motor is installed above the guide rail sliding table and drives the lead screw to rotate. The controller and the direct-current power supply are respectively connected with the driver, and the driver is connected with the motor. The direct-current power supply provides power for the motor. The radiation source controlleris connected with the radiation source.

The invention discloses a chemiluminescence immunoassay kit for quantitative detection of a soluble intercellular adhesion molecule-1 and a detection method. The kit contains a 96-well plate which is pre-coated with an anti-intercellular adhesion molecule-1 monoclonal antibody and the following bottled reagents: a phosphate wash solution, a blocking solution, a sample dilution solution, an intercellular adhesion molecule-1 standard solution, a horseradish peroxidase-labeled anti-intercellular adhesion molecule-1 polyclonal antibody IgG and a luminescent substrate, namely a luminal luminescent solution and quality control serum. The kit disclosed by the invention has the advantages of accurate and objective determination result of the soluble intercellular adhesion molecule-1 in human serum, simple and convenient method and easiness in popularization and application. The kit can reflect the autoimmune state in a patient with autoimmune thyroid disease can be used as a reference index for diagnosis of the autoimmune thyroid disease, and further has important significance for understanding the autoimmune active degree, predicting the occurrence of Graves disease and the recurrence of Graves hyperthyroidism, and determining reasonable treatment source, drug withdrawal time and other aspects.

The invention discloses a gas chromatography detecting method for the content of nonylphenol polyoxyethylene ether in a tanning agent, and relates to the field of analysis and testing. The method includes the steps of sample dissolution, chemical degradation treatment of sample liquid, purification by a solid-phase extraction column, gas chromatography detecting and comparison with a standard working curve to obtain the concentration value of nonylphenol polyoxyethylene ether. The method is convenient to operate, low in cost, high in reproducibility and accurate in qualitative process, and thecontent of nonylphenol polyoxyethylene ether in the tanning agent can be accurately detected without the aid of expensive gas chromatography-mass spectrometers or liquid chromatography-mass spectrometers. Compared with an existing testing technology, the method is low in cost and accurate and reliable in result when the concentration of nonylphenol polyoxyethylene ether in the tanning agent is detected.

The invention discloses a quick detection method for protein in milk. The method comprises the following steps: (1) preparing a sunset yellow solution as a detecting reagent; (2) preparing a series of standard protein aqueous solutions in concentrations, mixing the standard protein aqueous solutions with the detecting reagent, oscillating, centrifuging, taking supernate and diluting with water, preparing a standard color gradation card according to the solution color or using an ultraviolet and visible spectrophotometer for determining absorbancy at 483nm and drawing a standard curve; (3) mixing a to-be-detected milk sample with the detecting reagent, oscillating, centrifuging, taking supernate and diluting with water, comparing with the standard color gradation card or determining absorbancy at 483nm using the ultraviolet and visible spectrophotometer and calculating the content of the protein in the to-be-detected milk sample according to the standard curve. According to the method disclosed by the invention, the quick detection for the content of the protein in milk can be completed within 15-20min. When the method is adopted together with an ultraviolet spectrophotometer, the quantitative determination for the content of the protein in milk can be realized.

The invention relates to a coal / shale adsorption capacity measuring device, which comprises an adsorption cylinder and a reference cylinder, wherein cylinder bottoms of the two cylinders are respectively fixed on the top surface and the bottom surface of a cylindrical steel block; the adsorption cylinder and the reference cylinder are communicated through a communicating pipe which penetrates through the two cylinder bottoms and the cylindrical steel block; a first control valve is arranged on the communicating pipe; outer side walls of the adsorption cylinder, the reference cylinder and the cylindrical steel block are provided with heating jackets, and heat insulation jackets are arranged at outer sides of the heating jackets; all the combined members, namely the adsorption cylinder, the reference cylinder, the cylindrical steel block, the heating jackets and the heat insulation jackets, are placed in a vacuum heat insulation tank; a sample hanging basket which is filled with a test sample is arranged in the adsorption cylinder; the adsorption cylinder is provided with an exhaust pipe and a first pressure sensor; and the reference cylinder is provided with an air inlet pipe and a second pressure sensor, wherein the air inlet pipe is connected to one end of a gas boosting pump, and the other end of the gas boosting pump is communicated with experiment gas and helium through a second control valve and a third control valve which are communicated in parallel. The coal / shale adsorption capacity measuring device can be used for simulating the process of adsorbing natural gas by coal / shale under actual geological conditions and evaluating the adsorption capacity of the coal / shale.