The invention discloses a preparation method of copper foil electricity-conducting, heat-conducting, anti-shock and shielding adhesive tape. The preparation method includes the following steps that S1, copper foil is selected and prepared into a base material layer; S2, 20-30 parts by weight of nano-scale metal powder and 10-15 parts by weight of nano-scale graphite powder are mixed, then 40-60 parts by weight of methylbenzene is added, after the materials are stirred for 5-10 min, 10 wt% to 15 wt% of electricity-conducting and heat-conducting liquid is added, the materials are stirred for 15-20 min, and an electricity-conducting and heat-conducting adhesive is prepared; S3, a release material is selected and prepared into a release material layer, the release material layer is coated with the electricity-conducting and heat-conducting adhesive through a coating machine, then the release material layer is cured in an oven at the temperature of 80-100 DEG C for 5-10 min, the electricity-conducting and heat-conducting adhesive is cured into electricity-conducting and heat-conducting adhesive layers, the electricity-conducting and heat-conducting adhesive layers are transferred to the two faces of the base material layer, and the copper coil double-faced adhesive tape with the two sides provided with the release material layers is prepared. The structure is simple, an omni-bearing electricity-heating sponge layer and the electricity-conducting and heat-conducting adhesive layers are arranged, and the electricity-conducting, heat-conducting, anti-shock and shielding effects can be achieved.