55results about How to "Prevent excessive aggregation" patented technology

The present invention provides: a modified FcRn-binding domain having an enhanced affinity for the Fc Receptor neonatal (FcRn) at neutral pH; an antigen-binding molecule comprising said FcRn-binding domain, which has low immunogenicity, high stability and form only a few aggregates; a modified antigen-binding molecule having an increased FcRn-binding activity at neutral or acidic pH without an increased binding activity at neutral pH for a pre-existing anti-drug antibody; use of the antigen-binding molecules for improving antigen-binding molecule-mediated antigen uptake into cells; use of the antigen-binding molecules for reducing the plasma concentration of a specific antigen; use of the modified FcRn-binding domain for increasing the total number of antigens to which a single antigen-binding molecule can bind before its degradation; use of the modified FcRn-binding domain for improving pharmacokinetics of an antigen-binding molecule; methods for decreasing the binding activity for a pre-existing anti-drug antibody; and methods for producing said antigen-binding molecules.

The present invention relates to an oxynitride phosphor comprising an α-sialon as the main component, which is represented by the general formula: MxSi12−(m+n)Al(m+n)OnN16−n:Lny (wherein 0.3≦x+y<1.5, 0<y<0.7, 0.3≦m<4.5, 0<n<2.25, and assuming that the atomic valence of the metal M is a and the atomic valence of the lanthanide metal Ln is b, m=ax+by) and in which the aggregation index, A1=D50 / DBET≦3.0 or the aggregation index A2=D50 / Dparticle≦3.0; and a production method and usage of the phosphor.The phosphor of the present invention has less aggregation and a narrow particle size distribution, and therefore is easy to uniformly mix with a resin or the like, and a high-brightness white LED can be easily obtained.D50 [μm]: The median diameter in the grain size distribution curve.DBET [μm]: The equivalent-sphere diameter calculated on the basis of a BET specific surface area.Dparticle [μm]: The primary particle diameter measured by the image analysis of a scanning electron micrograph.

To provide a sialon-based phosphor capable of forming a thin film by mixing it with a resin, and giving a uniform photoluminescent light with high light emission intensity, and to provide a method for producing a sialon-based phosphor not having excessively large agglomerated particles and a wide particle size distribution, there is provided a method for producing an α-sialon based phosphor represented by the formula:MxLnySi12−(m+n)Al(m+n)OnN16−nwherein M is at least one metal selected from Ca, Mg, Y and Li, Ln is at least one lanthanide metal selected from Eu, Dy, Er, Tb, Yb and Ce, ax+by=m (assuming that the valence of metal M is a and the valence of lanthanide metal Ln is b), 0<x≦1.5, 0.3≦m≦4.5, and 0<n<2.25), wherein the silicon nitride raw material used as the raw material is a silicon nitride raw material powder comprising amorphous silicon nitride and crystalline silicon nitride.

A cell culture product is provided for propagating embryonic stem cells, and maintaining their self-renewal and pluripotency characteristics for extended periods of time in culture. The cell culturing, product includes a substrate; and a coating thereon deposited, from a coating solution. The coating solution includes a mixture of extracellular matrix proteins and an aqueous solvent, wherein the total protein concentration in the coating solution is about 10 μg / ml to about 1 mg / ml.

This invention is a method of minimizing the variation of cell growth and production through homogeneous cell line development. To be more specific, it is the method of isolating and proliferating single cell clone from cambium to promote the stability of the plant-derived biologically active substances production by solving the problems of decrease in cell growth and the productivity during the long term culture.

A cell culture medium with high content of choline chloride is provided. The cell culture media further comprise only moderate amounts of amino acids, in particular the amount of glutamine in the cell culture media is limited. The cell culture media can be used for large scale production of polypeptides using cell cultures. The cell culture media with high content of choline chloride are particularly suitable for fed-batch cell culture whereby cell viabilities stay at a higher level for a longer time and high polypeptide titers although limited amounts of amino acids are used.

A dispersion of metal oxide fine particles, containing metal oxide fine particles, a strong acid and an aqueous solution, wherein the metal oxide fine particles and the strong acid are dispersed in an aqueous solution containing alcohol, and the dispersion of metal oxide fine particles has a light transmittance at 800 nm wavelength of 90% or more.

This invention is a method of minimizing the variation of cell growth and production through homogeneous cell line development. To be more specific, it is the method of isolating and proliferating single cell clone from procambium to promote the stability of the plant-derived biologically active substances production by solving the problems of decrease in cell growth and the productivity during the long term culture.

The object is to provide an enriched liquid food which comprises a soybean protein material, which has a low viscosity, which produces little aggregates, and which has good physical properties. An enriched liquid food can be prepared by using a soybean protein material having the following physical properties a) to d): a) an aqueous solution containing the soybean protein material in an amount of 5 g in terms of solid content relative to 100 g of the aqueous solution and also containing calcium lactate in an amount of 30 mg in terms of calcium content has a viscosity of 10 mPa·s or less at 20° C. after the aqueous solution is heated at 121° C. for 10 minutes, and produces precipitates in an amount of 1 vol % or less after the aqueous solution is centrifuged at 1,300×g for 5 minutes; b) a 12-wt % aqueous solution of the soybean protein material has a viscosity of 1,000 mPa·s or less at 5° C.; c) the soybean protein material can be solubilized in a 0.22 M TCA at a solubilization ratio of less than 11%; and d) the soybean protein material has a PSI (protein solubility index) value of 70 or more. The soybean protein material can be produced by heating an aqueous solution or an aqueous dispersion solution of a soybean protein raw material at pH 5.7 to 7.4 and at a temperature of 110 to 160° C. for 15 to 70 seconds.

The present invention concerns antibody-conjugate having general structure (2):AB-[(L6)-{Z-(L1)n-(L2)o-(L3)p-(L4)q-D}xx]yy   (2)wherein AB is an antibody capable of targeting Trop-2-expressing tumours and D is selected from the group consisting of taxanes, anthracyclines, camptothecins, epothilones, mytomycins, combretastatins, vinca alkaloids, maytansinoids, enediynes such as calicheamicins, duocarmycins, tubulysins, amatoxins, bleomycins, dolastatins and auristatins, pyrrolobenzodiazepine dimers, indolinobenzodiazepine dimers, radioisotopes, therapeutic proteins and peptides (or fragments thereof), kinase inhibitors, MEK inhibitors, KSP inhibitors, and analogues or prodrugs thereof. These antibody-conjugates exhibit an improved therapeutic index. The invention further concerns a process for preparing the antibody-conjugate according to the invention, a method for targeting Trop-2-expressing cells, medical uses of the antibody-conjugates according to the invention.

Platinum particles have been formed as porous, hollow tubular dendrites by using silver dendrite particles in a galvanic replacement reaction conducted in an aqueous solution of a platinum compound. The dendritic platinum particles have been found useful as catalysts and particularly useful as a hydrogen-oxidation electrocatalyst and / or an oxygen-reduction catalyst in a polymer electrolyte membrane fuel cell.

A cell culture medium with high content of choline chloride is provided. The cell culture media further comprise only moderate amounts of amino acids, in particular the amount of glutamine in the cell culture media is limited. The cell culture media can be used for large scale production of polypeptides using cell cultures. The cell culture media with high content of choline chloride are particularly suitable for fed-batch cell culture whereby cell viabilities stay at a higher level for a longer time and high polypeptide titers although limited amounts of amino acids are used.