The invention provides a preparation method of
sunflower seed polypeptide, comprising the following steps: a,
processing of raw materials: uniformly mixing water and a
sunflower seed cake crushed material, which is obtained after a low-temperature cold-pressed
sunflower seed cake is crushed, so as to obtain a
sunflower seed cake suspension; b, homogenization of the suspension: homogenizing the
sunflower seed cake suspension to prepare a
sunflower seed cake mixed liquor; c, thermal-insulation
enzymatic hydrolysis: adding a mixed the sunflower seed cake mixed
enzyme liquid of AlcaLase
enzyme and
papain into the sunflower seed cake mixed liquor to carry out
enzymatic hydrolysis at the temperature of 50-60 DEG C at pH of 7.0-8.5 for 6-8h so as to prepare a sunflower seed cake enzymatic
hydrolysate; d, high temperature sterilization: rapidly heating the sunflower seed cake enzymatic
hydrolysate to 90-95 DEG C; e, liquid-
solid separation: carrying out liquid-
solid separation on the sunflower seed cake enzymatic
hydrolysate which has undergone high-temperature
enzyme deactivation and removing insoluble solids so as to prepare a sunflower seed polypeptide liquid; f, condensation: condensing the sunflower seed polypeptide liquid to prepare a sunflower seed polypeptide concentrate; and g,
drying:
drying the sunflower seed polypeptide concentrate so as to obtain a sunflower seed polypeptide
powder.