The invention discloses a sensitive probe as well as a preparation method and a method for detecting
salmonella enteritidis by virtue of the sensitive probe, relates to a probe, a preparation method and application of the probe, and aims to solve the problems of high cost,
instability, high deactivation rate of an
antibody and poor sensitivity of an existing probe. The sensitive probe is prepared from
antibiotics and a
signal carrier. The preparation method comprises the following steps: 1, preparing yellowish-brown
emulsion; 2, preparing a carboxylated
immunomagnetic bead; 3, preparing an activated magnetic
microsphere; 4, adding a resuspension solution for storage. The method for detecting the
salmonella enteritidis by virtue of the sensitive probe comprises the following steps: 1, preparing an
immunochromatographic test strip for the
salmonella enteritidis; 2, mixing liquid to be detected and the sensitive probe for incubation, adding dropwise a
mixed solution onto a sample pad of the
immunochromatographic test strip for the
salmonella enteritidis, performing reaction for 10 minutes, and reading a result. According to the sensitive probe, the preparation method and the detection method, only one
antibody is struck on a
nitrocellulose membrane for direct detection, so that the cost is greatly reduced, and simplicity and convenience are achieved; the sensitive probe, the preparation method and the detection method are applied to detection of the
salmonella enteritidis.