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Sensitive probe as well as preparation method and method for detecting salmonella enteritidis by virtue of sensitive probe

A Salmonella Enteritidis and probe detection technology, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of expensive probes, easy inactivation of antibodies, sensitivity, instability, etc., and achieves simple structure, good optical properties, and cost-effectiveness. high effect

Active Publication Date: 2017-12-15
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of expensive and unstable existing probes, easy inactivation of antibodies and poor sensitivity, and provide a sensitive probe and its preparation method and its method for detecting Salmonella enteritidis

Method used

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  • Sensitive probe as well as preparation method and method for detecting salmonella enteritidis by virtue of sensitive probe
  • Sensitive probe as well as preparation method and method for detecting salmonella enteritidis by virtue of sensitive probe
  • Sensitive probe as well as preparation method and method for detecting salmonella enteritidis by virtue of sensitive probe

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specific Embodiment approach 1

[0060] Embodiment 1: This embodiment is a sensitive probe, which is prepared from an antibiotic and a signal carrier; the antibiotic is ampicillin; and the signal carrier is carboxylated immunomagnetic beads.

specific Embodiment approach 2

[0061] Embodiment 2: This embodiment differs from Embodiment 1 in that: the particle size of the carboxylated immunomagnetic beads is 150nm-200nm. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0062] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the carboxylated immunomagnetic beads are specifically prepared according to the following steps:

[0063] 1. FeCl 3 ·6H 2 Add O and trisodium citrate dihydrate into ethylene glycol, then dissolve in an air-bath shaker at a temperature of 36°C to 37°C and a speed of 200r / min to 300r / min for 30min to 60min, then add sodium acetate, Dissolve in an air bath shaker at a temperature of 36°C-37°C and a rotation speed of 200r / min-300r / min for 30min-60min to obtain a yellow-brown emulsion;

[0064] FeCl as described in step 1 3 ·6H 2 The volume ratio of the mass of O to ethylene glycol is (0.5g~1g):(10mL~30mL);

[0065] FeCl as described in step 1 3 ·6H 2 The mass ratio of O and trisodium citrate dihydrate is (0.5~1):(0.1~0.4);

[0066] FeCl as described in step 1 3 ·6H 2 The mass ratio of O and sodium acetate is (0.5~1):(1~2);

[0067] 2. Transfer the yellow-brown emulsion obtained in ...

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Abstract

The invention discloses a sensitive probe as well as a preparation method and a method for detecting salmonella enteritidis by virtue of the sensitive probe, relates to a probe, a preparation method and application of the probe, and aims to solve the problems of high cost, instability, high deactivation rate of an antibody and poor sensitivity of an existing probe. The sensitive probe is prepared from antibiotics and a signal carrier. The preparation method comprises the following steps: 1, preparing yellowish-brown emulsion; 2, preparing a carboxylated immunomagnetic bead; 3, preparing an activated magnetic microsphere; 4, adding a resuspension solution for storage. The method for detecting the salmonella enteritidis by virtue of the sensitive probe comprises the following steps: 1, preparing an immunochromatographic test strip for the salmonella enteritidis; 2, mixing liquid to be detected and the sensitive probe for incubation, adding dropwise a mixed solution onto a sample pad of the immunochromatographic test strip for the salmonella enteritidis, performing reaction for 10 minutes, and reading a result. According to the sensitive probe, the preparation method and the detection method, only one antibody is struck on a nitrocellulose membrane for direct detection, so that the cost is greatly reduced, and simplicity and convenience are achieved; the sensitive probe, the preparation method and the detection method are applied to detection of the salmonella enteritidis.

Description

technical field [0001] The invention relates to a probe, a preparation method and an application of the probe. Background technique [0002] Bacterial infectious diseases account for one-third of all deaths worldwide and remain a major public health concern. Therefore, early identification of bacterial infections through point-of-care detection (POC) is crucial for the safety of water, food and clinical diagnosis. Immunochromatography test strips, due to their advantages of convenient preparation and operation, low cost, one-time use, short detection time, intuitive and reliable results, etc., do not require special equipment, and are the most common instant detection and diagnostic tools for various Detection of proteins, antibiotics, metal ions, toxins, etc. Typical sandwich test strips use specific biological components, such as antibodies, based on the principle of antigen-antibody specific binding, the target molecule in the sample reacts with the gold-labeled antibod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/543
Inventor 王建龙张道宏补彤闫灵芝黄琼窦磊娜赵兵欣赵东阳
Owner NORTHWEST A & F UNIV
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