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30results about How to "Maintain sustainable development" patented technology

Indirect competition enzyme linked immunoreagent kit for detecting lead ions and manufacturing method thereof

InactiveCN103472230AResolving the immunogen <u>sex</u> questionHigh potencyMaterial analysisAbzymeAntigen
The invention relates to an indirect competition enzyme linked immunoreagent kit for detecting lead ions and a manufacturing method of the kit. An elisa plate enveloped by lead ion envelope antigens, lead-ion monoclonal antibodies, elisa second antibodies, a substrate color developing solution, a stop solution, a lead ion standard solution, a washing liquor concentrated solution and a sample treating liquid are arranged in the kit. The indirect competition enzyme linked immunoreagent kit can detect the lead irons in a trace mode, is used for detecting the lead iron contamination residual in the environment, the soil, water, foods and containers, and has the advantages of being rapid, easy and convenient to use, sensitive, peculiar, economical and the like. The kit is few in detection step and high in timeliness, saves detection time, reduces operation errors and can perform field detection. The kit not only can be used for screening samples in large batch, but also can perform rapid detection on samples in small batch, and provides effective means for food import and export inspection, food inspection, monitoring and evaluation of environmental pollution and the like.
Owner:HENAN INST OF SCI & TECH

Brown planthopper N1MLP gene, encoded protein and application of brown planthopper NIMLP

ActiveCN106754948AFunctions that affect growth and developmentReduced survival rateBiocideAnimal repellantsBrown planthopperObserved Survival
The invention discloses a brown planthopper sialaden secretor gene N1MLP and an application OF brown planthopper NIMLP in paddy resisting against brown planthopper after the gene N1MLP is restrained. A complete cDNA sequence of the brown planthopper sialaden secretor gene N1MLP is shown as SEQ ID No.1; ds RNA of the N1MLP enters the brown planthopper in the manner of micro-injection, so that the N1MLP gene expression quantity is reduced, the feeding of the brown planthopper is affected and the survival rate of the brown planthopper is reduced; the ds RNA of the N1MLP is stably expressed in the paddy plant; after the brown planthopper eats the paddy, the N1MLP gene expression quantity is reduced, so that the brown planthopper weight is reduced and the survival rate of the brown planthopper is reduced. According to the invention, the N1MLP, of which the expression is restrained, has significance in researching the paddy resisting against brown planthopper and preventing and treating the brown planthopper in agricultural production.
Owner:WUHAN UNIV

Indirect competition enzyme linked immunoreagent kit for detecting mercury ions and manufacturing method thereof

InactiveCN103472231AHigh potencyHigh water titerMaterial analysisAbzymeAntigen
The invention relates to an indirect competition enzyme linked immunoreagent kit for detecting mercury ions and a manufacturing method of the kit. An elisa plate enveloped by mercury ion envelope antigens, mercury-ion-resisting monoclonal antibodies, elisa second antibodies, a substrate color developing solution, a stop solution, a mercury ion standard solution, a washing liquor concentrated solution and a sample treating liquid are arranged in the kit. The kit can detect the mercury irons in a trace mode, is used for detecting the mercury irons in the environment, the soil, water, foods, medicines, cosmetics and the like, and has the advantages of being rapid, easy and convenient to use, sensitive, peculiar, economical and the like. The kit is few in detection step and high in timeliness, saves detection time, reduces operation errors and can perform field detection. The kit is low in requirement for pretreating samples, simple in treatment process, not only can be used for screening the samples in large batch, but also can perform rapid detection on the samples in small batch, and not only provides technical support for environment and food safety, but also provides effective technological means for food import and export inspection, food inspection, monitoring and evaluation of environmental pollution and the like.
Owner:ZHENGZHOU UNIV

Streptomyces hygroscopicus capable of producing salicylic acid and rapamycin and application of streptomyces hygroscopicus to prevention and control of plant oomycetes and fungal diseases

The invention discloses streptomyces hygroscopicus capable of producing salicylic acid and rapamycin and application of the streptomyces hygroscopicus to prevention and control of plant oomycetes andfungal diseases. The invention also discloses the streptomyces hygroscopicus capable of producing the salicylic acid and the rapamycin, which has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 15518, and the application of the streptomyces hygroscopicus to the prevention and control of the plant oomycetes and fungal diseases. The invention identifiesa biocontrol bacterium aiming at the plant oomycetes and fungal diseases, especially phytophthora infestans and fusarium oxysporum; the bacterium can be used for remarkably inhibiting spore germination and mycelium growth of the phytophthora infestans; an effective method is provided for biologically preventing and controlling the plant oomycetes and fungal diseases, especially the phytophthora infestans and fusarium wilt. The method disclosed by the invention can be used for effectively preventing and controlling the phytophthora infestans, the fusarium wilt and other plant diseases and reducing the economic loss; the method has important meaning of reducing the utilization of pesticide and alleviating environment pollution, and also has significant application value of keeping the balance and sustainable development of an ecological system.
Owner:CHONGQING UNIV

Indirect competitive enzyme linked immunosorbent assay kit for detecting chromium ions as well as preparation and detection methods thereof

The invention relates to an indirect competitive enzyme linked immunosorbent assay (elisa) kit for detecting chromium ions as well as preparation and detection methods thereof. A trivalent chromic ion envelope antigen-enveloped elisa plate, a trivalent chromic ion-resistant monoclonal antibody, a second enzyme-labeled antibody, a substrate color-developing solution, a stop solution, a trivalent chromic ion standard solution, a lotion concentrated solution and a sample processing solution are arranged in the kit. Samples are processed before detection so that Cr3<+> in the samples forms Cr<3+>-EDTA (ethylene diamine tetraacetic acid), and then the chromium ions are detected by the kit. The chromium ion enzyme linked immunosorbent assay kit can be used for detecting the trace amount of chromium ions, has the Cr<3+> detection sensitivity of 2ng / ml, has the characteristics of quickness, simplicity and convenience, sensitivity, specificity, economy and the like, has few detection steps, saves the detection time, and reduces the operation error; the detection cost is less than 1 / 20 of that of a physical and chemical analysis method, the timeliness is high, the field detection can be performed, and the kit is mainly used for screening large-batch samples of chromium ion pollution residues in environments, soil, water and foods.
Owner:HENAN INST OF SCI & TECH

Tomato production method and antimicrobial composition

The invention relates to a tomato production method and an antimicrobial composition. The tomato production method comprises the following points: (a) ecological restoration is carried out, wherein ecological restoration comprises one or more of the following steps: (a1) an organic fertilizer and a biological microbial fertilizer are applied; (a2) crop straws are used for covering between lines; (a3) grass is allowed to grow during slack periods; and (a4) crop rotation is carried out; (b) water and fertilizer applications are controlled, wherein fertilizer types and amounts are determined according to different tomato growth and development stages; and (d) pest and disease control is carried out, wherein herbivorous creature avoidance plants are intercropped in the field, and beneficial creatures are cultured. With the method, farmland ecosystem stability and sustainable development can be maintained; and commercial tomato appearance quality, flavor quality and safety quality can be improved.
Owner:郭强

Big fixed net cage for cultivating seawater fishes and using method thereof

The invention belongs to the field of seawater cultivation engineering and particularly discloses a big fixed net cage for cultivating seawater fishes and a using method thereof. The big fixed net cage for cultivating the seawater fishes and the using method thereof solve the problems of poor anti-typhoon capability, more diseases, high making cost and the like of the current net cages. Piles of the net cage are distributed along the periphery; a net encloses the piles to form a cage structure; the upside and the lower side of the net are coiled by an upper net cable and a lower net cable; the tops of the piles are connected with diagonal tension cables II; the upper parts of the piles are connected with diagonal tension cables I; and the tops of the corresponding piles are connected by transverse cables. By piling piles under the sea and providing the transverse cables, the diagonal tension cables, the upper and lower cables of the nets to fix the big nets, the cultivating capacity of the net cage is improved, the cultivating cost is reduced, the quality of the cultivated fishes is improved and the cultivating environment is protected. The big fixed net cage for cultivating the seawater fishes and the using method thereof make a full use of and maintain a good ecological environment for cultivation, produce the highly qualified seawater fishes, prevent the cultivating area from being polluted and prevent the occurrence of diseases in the aquaculture, improve the living environment for the cultivated fishes and reduce losses due to natural disasters.
Owner:宁德市登月水产食品有限公司

New application of sirolimus in inhibition of plant alternaria solani

The invention discloses application of sirolimus in inhibition of plant alternaria solani and further discloses a fungicide. The fungicide contains rapamycin or a mixture of the rapamycin and a chemical fungicide. A TOR protein inhibitor rapamycin used in clinical medicine is used for prevention and control research of plant early blight for the first time, it is found through the research that the rapamycin and the mixture of the rapamycin and the chemical fungicide can effectively inhibit the plant alternaria solani, a chemical rapamycin pesticide can play a synergistic effect to inhibit the plant alternaria solani, a very significative base is provided for research of a novel drug for preventing and controlling the early blight, the novel fungicide is wide in application prospect and market prospect, a new choice is provided for early blight prevention and control, the using amount of the chemical pesticide can be remarkably decreased, and the fungicide has the great significance on reduction of pesticide usage amount and delay of the resistance of insect pests and also has the great application value on maintenance of ecosystem balance and sustainable development.
Owner:CHONGQING UNIV

Method for purifying reverse osmosis concentrated water with active carbon-membrane separation countercurrent absorption

The invention discloses a method for purifying reverse osmosis concentrated water with an active carbon-membrane separation accumulation countercurrent absorption technology. The method comprises the following steps of: extracting concentrated drain water produced in the running process of a reverse osmosis device, and adding into a membrane reactor for undergoing an adsorption reaction; dissolving powder active carbon (PAC) into the membrane reactor for continually adsorbing, wherein the adding amount of the PAC is determined according to the content of organic matters in the reverse osmosis concentrated water, and the adding amount of the PAC can be added appropriately if a treatment effect is poor; and performing aerated stirring and circulating reflux, adsorbing for 10-15 minutes, uniformly dispersing the PAC, fully mixing with reverse osmosis concentrated water, and discharging purified water with a hollow fiber membrane assembly. PAC accumulation countercurrent absorption and membrane separation are used together, so that cost is low, operation is easy and convenient, and automatic degree is high. Due to the adoption of the method for purifying reverse osmosis concentrated water, the requirements of the Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant are met, and the potential hazard of direct discharge on a water environment is reduced; and the method plays an important role in maintaining the balance of a water ecological system.
Owner:TIANJIN UNIV

New application of TOR protein inhibitor to inhibition of wilt fusarium and verticillium dahliae of plants

InactiveCN105794803ASuppresses Fusarium WiltReduce usageBiocideDead animal preservationBiotechnologyVermin
The invention discloses application of a TOR protein inhibitor to inhibition of wilt fusarium and verticillium dahliae of plants and a fungus bactericide. The fungus bactericide contains the TOR protein inhibitor, wherein the TOR protein inhibitor is Rapamycin, Torin 1, a mixture of Rapamycin and Torin 1, or a mixture of Rapamycin and a fungus chemical bactericide. According to the application disclosed by the invention, the TOR protein inhibitor used for clinical medicine is used for prevention and treatment researches of the wilt fusarium and the verticillium dahliae of the plants for the first time; researches show that the TOR protein inhibitor including Rapamycin, Torin 1, the mixture of Rapamycin and Torin 1 and the mixture of Rapamycin and the fungus chemical bactericide can be used for effectively inhibiting the wilt fusarium and the verticillium dahliae of the plants, so that a new choice for preventing and treating wilt and verticillium wilt is provided, and has very important meanings on reducing the utilization of pesticides and delaying the insect resistance.
Owner:CHONGQING UNIV

Application of glutathione waste liquid in microorganism production and application method

The invention discloses application of glutathione waste liquid in microorganism production and an application method and belongs to the field of microorganisms. The glutathione waste liquid is used as the effective constituent of a seeding tank culture medium and / or a fermentation tank culture medium to be applied to the microorganism production, good social and economic values are achieved, microorganism preparation with good performance can be obtained, and wide application value is achieved.
Owner:JINAN YIBANG BIOTECH CO LTD

Indirect competitive enzyme linked immunosorbent assay kit for detecting chromium ions as well as preparation and detection methods thereof

The invention relates to an enzyme-linked immunoassay kit for detecting chromium ions and its assembly and detection method. The kit contains an ELISA plate coated with trivalent chromium ion-coated antigen, anti-trivalent chromium ion monoclonal antibody, enzyme-labeled secondary antibody, substrate chromogenic solution, stop solution, and trivalent chromium ion standard solution , lotion concentrate, sample treatment solution. Sample processing is carried out before detection, so that Cr3+ in the sample forms Cr3+-EDTA, and then the kit is used for detection. The chromium ion ELISA kit of the present invention can detect trace amounts of chromium ions, and the sensitivity of detecting Cr3+ is 2ng / mL. Operation error; the detection cost is less than 1 / 20 of the physical and chemical analysis method, and the timeliness is strong, and on-site detection can be carried out. It is mainly used for the screening of large batches of samples with chromium ion contamination residues in the environment, soil, water, and food.
Owner:HENAN INST OF SCI & TECH

Plastic film residue pickup machine

The invention discloses a plastic film residue pickup machine. The plastic film residue pickup machine comprises a soil shoveling plate, a pickup vehicle body, a wind power generation device, first air outlet devices and a plastic film residue collection box, wherein an inlet is formed in the front end of the pickup vehicle body, an outlet is formed in the rear end, the soil shoveling plate is obliquely arranged in front of the pickup vehicle body, the high end of the soil shoveling plate is connected with the inlet of the pickup vehicle body, a soil conveying part and a stirring part are arranged in the pickup vehicle body, the soil conveying part is arranged in the direction from the inlet to the outlet, the stirring part is arranged above the soil conveying part, the plastic film residue collection box is arranged at the top of the pickup vehicle body, the first air outlet devices are arranged in front of the stirring part and connected with the wind power generation device through air pipes, air flows out of the first air outlet devices in the direction blowing from the stirring part to the plastic film residue collection box, and air inlets are formed in corresponding positions of the plastic film residue collection box. The problems that a conventional plastic film residue recovery machine cannot clean and recover plastic film residues in deep soil, cleans and recovers the plastic film residues in shallow soil incompletely and requires manual secondary cleaning are solved.
Owner:YUNNAN TOBACCO CORP QUJING BRANCH

Enzyme-linked immunoassay kit for detecting hexavalent molybdenum ions and its composition and detection method

The invention relates to an enzyme linked immunosorbent assay (ELISA) kit for detecting hexavalent molybdenum ions as well as establishment and detection methods thereof. The kit is internally provided with an ELISA plate covered by a hexavalent molybdenum ion coating antigen, a hexavalent molybdenum ion resisting monoclonal anti-antibody, an enzyme-labeled second antibody, a primer developing solution, a stopping solution, a hexavalent molybdenum ion standard solution, a concentrated washing solution and a sample treating solution. Before the detection, 0.1mol / L EDTA (Ethylene Diamine Tetraacetic Acid) chelating agent is used for treating a sample, the hexavalent molybdenum ions and the EDTA are sufficiently chelated to obtain a sample solution to be detected, and then the solution is detected. The kit provided by the invention can detect the trace hexavalent molybdenum ions and the detection sensitivity is 1ng / mL; detection steps are few so that the time is saved, the operation error is reduced and the detection cost is not more than 1 / 20 of that of a physical and chemical analysis method; the timeliness is strong and field detection can be carried out; the kit has the properties of rapidness, simplicity, convenience, sensitivity, specificity, economical efficiency and the like, and is mainly used for sieving large-batch samples polluted by residual molybdenum ions in the environment, soil, water and foods.
Owner:HENAN INST OF SCI & TECH

Application of brown planthopper PIB14 protein and coding gene thereof to regulation and control of brown planthopper resistance of plants

ActiveCN113248586AFunctions that affect growth and developmentReduced survival rateFermentationAnimals/human peptidesBiotechnologyBrown planthopper
The invention relates to the technical field of genetic engineering, in particular to application of a brown planthopper PIB14 protein and a coding gene thereof to regulation and control of brown planthopper resistance of plants. According to the invention, the brown planthopper PIB14 protein and a sequence of the coding gene thereof are found, and the gene has the great significance for regulating and controlling the resistance of plants to brown planthoppers and regulating and controlling the growth of the brown planthoppers. The viability of the brown planthopper can be effectively reduced by inhibiting expression of the PIB14 gene in the brown planthopper, the resistance of the plants to the brown planthopper can be effectively improved by overexpressing the PIB14 gene in the plants, and the brown planthopper PIB14 protein and the coding gene thereof found by the invention have very good application prospect, and have high application value of reducing the use of pesticides and maintaining ecological balance and sustainable development.
Owner:WUHAN UNIV +2

Chromium ion rapid detection gold standard test paper and its preparation method and application

InactiveCN103412125BFast detection of chromium ionsShorten detection timeMaterial analysisCellulosePhysical chemistry
The invention discloses a gold-labeled test strip for rapid detection of chromium ions as well as a preparation method and an application thereof. The gold-labeled test strip is characterized in that a bottom layer is a support layer, a middle layer is an absorption layer, a protection film is fixed on the absorption layer, the absorption layer is sequentially provided with a sample pad, a gold-labeled antibody binding pad, and a cellulose membrane layer from a test end as well as a water absorption pad at a handle end, detection prints printed by a carrier protein solution coupled with the chromium ions are arranged on the cellulose membrane layer, and contrast prints printed by rabbit-anti-mouse or goat-anti-mouse IgG (Intravenous Gamma Globulin) antibody solution are arranged on the cellulose membrane layer; colloidal gold-labeled chromium ion monoclonal antibodies are coated in the gold-labeled antibody binding pad. The gold-labeled test strip can be used for rapidly detecting pollution residues of the chromium ions in soil, water and food, has the advantages of specificity, sensitivity, rapidness, simplicity, convenience, visual and intuitional result and the like, not only can be used for screening large-batch samples, but also can be used for rapidly detecting small-batch samples and is wide in applicable range.
Owner:HENAN INST OF SCI & TECH

RNAi expression vector of Plutella xylostella tor gene and its construction method and application

The invention discloses an RNAi (ribonucleic acid interference) expression vector for TOR (target of rapamycin) genes of plutella xylostella, a method for constructing the RNAi expression vector and application thereof. The RNAi expression vector comprises separate polynucleotide, a vector and a recombinant vector. Sequences of the separate polynucleotide are shown as SEQ ID NO:1 and SEQ ID NO:2; the vector comprises the polynucleotide with the sequences shown as SEQ ID NO:1 or SEQ ID NO:2; the recombinant vector comprises sequences shown as SEQ ID NO:7, and pEarleyGate 303 is used as a skeleton of the RNAi expression vector for the TOR genes of the plutella xylostella. The method for preparing the RNAi expression vector includes displacing and inserting EcoR I, AsiS I, P35S promoters, Not I, the sequences SEQ ID NO:1, Sbf I, fourth introns of ACTIN, BbvC I, reverse complement sequences of the sequences SEQ ID NO:1, Asc I, T35S terminators and EcoR I, which are sequentially connected with one another, in recombination sites.
Owner:CHONGQING UNIV

A secondary treatment method for sodium flue gas desulfurization liquid

The invention discloses a secondary treatment method of a flue gas desulfurization liquid treated with a sodium method. The secondary treatment method comprises operation steps as follows: the sodium-method desulfurization liquid is sent into a sulfur burning furnace to be burnt, and produced high-temperature gas and little sulfate are rapidly cooled by a quench cooler; the cooled gas and sulfate are mixed in the quench cooler and then enter a gas-liquid separation tank, a sulfate solution and gas are formed after vapor is condensed, gas obtained after gas-liquid separation is sucked away by a fan, the sulfate solution is treated by a sulfate solution cooler, passes by the quench cooler and returns to the gas-liquid separation tank, and the operation is cycled until the sulfate solution is saturated; the saturated sulfate solution is concentrated and crystalized by a sulfate multi-effect evaporation device or an MVR (mechanical vapor recompression) evaporation device, and waste recycling is realized. The secondary treatment method adopts reasonable process procedure design, has the advantages that the sewage treatment effect is good, the recycling rate is high, the operation is simple and convenient, system operation is stable and blocking is difficult to cause, and can be widely applied to factory treatment of the flue gas desulfurization liquid treated with the sodium method.
Owner:NANTONG SUNSHINE GRAPHITE EQUIP TECH

Transgenic euramerican populus trichocarpa*populus deltoides for resisting apocheima cinerarius pests

The invention discloses transgenic euramerican populus trichocarpa*populus deltoides for resisting apocheima cinerarius pests. The transgenic euramerican populus trichocarpa*populus deltoides is obtained by the following methods of taking total RNA (ribonucleic acid) of leaves of populus trichocarpa*populus deltoides (populus trichocarpa*populus deltoides) as a template, taking SEQ ID No.2,3 as primers, obtaining SEQ ID No. 1 by RT-PCR (reverse transcription-polymerase chain reaction) and being bonded to pJET1.2 to obtain recombinant plasmid and leading E.coli strain TOP10, screening positive clones, and screening bacterial colony containing resistance plasmids; carrying out PCR validation and double enzyme validation on extracted plasmids, choosing correct recombinant, naming as pBI121-WIN4 and transferring into agrobacterium tumefaciens to carry out colonial PCR verification, converting the obtained bacterial strain into euramerican populus trichocarpa*populus deltoides to obtain the transgenic euramerican populus trichocarpa*populus deltoides for resisting the apocheima cinerarius pests. The transgenic euramerican populus trichocarpa*populus deltoides for resisting apocheima cinerarius pests disclosed by the invention has a strong ability for resisting the apocheima cinerarius pests.
Owner:TIANJIN UNIV

Glazes made from tailings resources and their glazing process

The invention discloses a glaze material manufactured by utilization of tailings. The glaze material comprises 40-65% of feldspar, 10-25% of quartz, 15-30% of iron ore mine tailings, 0-10% of limestone, 0-10% of kaolin and 0-10% of bone ash. The glaze material comprises following components by weight: 1-5 parts of K2O, 1-8 parts of Al2O3, 55-70 parts of SiO2, 1-5 parts of P2O5, 1-5 parts of Na2O, 1-8 parts of Fe2O3, 5-15 parts of CaO and 1-8 parts of MgO. The invention also discloses a glazing process of the glaze material manufactured by utilization of the tailings. The glazing process includes: adding the materials into a ball grinder and performing ball-milling, adding water, uniformly mixing, stirring and dissolving to obtain glaze slurry, with the fineness of the glaze slurry being controlled to be less than 0.01% of residue on sieve of a ten-thousand hole sieve; discharging the glaze slurry and stirring; sieving the glaze slurry with a sieve having a size of 200 meshes, applying the fully suspended glaze slurry onto the surface of a green body with the concentration being 38-46 baume degrees and the thickness of the glaze slurry on the surface of the green body being 0.2-1.2 mm; and subjecting the green body to high-temperature sintering, and cooling in furnace to room temperature after the sintering is finished to obtain a ceramic product. By adoption of a one-time sintering process, the product quality can reach that of secondary sintering and the product energy consumption is reduced.
Owner:苏友谊

Method and system for no tampering based historical data to compensate exchange losses

PendingCN108711050ALong circulationImprove wealth distributionData miningCurrency conversionUser confidenceAnxiety
The invention provides a method and system for no tampering based historical data to compensate exchange losses. The method includes extracting historical data in a blockchain; performing data excavation according to the data; forming a compensation proposal according to calculation results; voting on proposals that are in need; and implementing compensation and recording. The system includes a historical data extracting module, a data excavation module, a proposal module, a voting module and an implementing module. Blockchain data has the characteristic of no tampering; and subsidies can be performed on users who accord with conditions and the users can be empowered by tracing back to history based on the characteristic of the blockchain. There are no specific constraints on subsidized behavior objects, the time and intensity of the subsidies cannot be known in advance, and thus, moral risks can be avoided, user confidence can be built, buying in or selling out by following the trendcaused by anxiety can be avoided, currency stability can be realized in the long run, and therefore, the ecological balance and sustainable development of blockchain systems can be maintained.
Owner:WUHAN LONGJIN SCI & TECH

New application of tor protein inhibitor to inhibit Fusarium wilt and Verticillium dahliae

The invention discloses application of a TOR protein inhibitor to inhibition of wilt fusarium and verticillium dahliae of plants and a fungus bactericide. The fungus bactericide contains the TOR protein inhibitor, wherein the TOR protein inhibitor is Rapamycin, Torin 1, a mixture of Rapamycin and Torin 1, or a mixture of Rapamycin and a fungus chemical bactericide. According to the application disclosed by the invention, the TOR protein inhibitor used for clinical medicine is used for prevention and treatment researches of the wilt fusarium and the verticillium dahliae of the plants for the first time; researches show that the TOR protein inhibitor including Rapamycin, Torin 1, the mixture of Rapamycin and Torin 1 and the mixture of Rapamycin and the fungus chemical bactericide can be used for effectively inhibiting the wilt fusarium and the verticillium dahliae of the plants, so that a new choice for preventing and treating wilt and verticillium wilt is provided, and has very important meanings on reducing the utilization of pesticides and delaying the insect resistance.
Owner:CHONGQING UNIV

Gold label test paper for rapidly detecting molybdic ions, well as preparation method and application thereof

InactiveCN103412126BFast detection of hexavalent molybdenum ionsShorten detection timeMaterial analysisCelluloseAntiendomysial antibodies
The invention discloses gold label test paper for rapidly detecting molybdic ions, as well as a preparation method and an application thereof. The bottom layer of the gold label test paper is a supporting layer, the middle layer of the gold label test paper is an adsorption layer, a protective film is fixedly arranged on the absorption layer, the absorption layer comprises a sample cushion, a gold label antibody combined cushion, a cellulose film layer and a handle-end absorbent cushion from a test end in sequence, and detection imprints printed by a carrier protein solution of coupling molybdic ions and contrast imprints printed by a rabbit anti-mouse IgG antibody solution are arranged on the absorbent cushion; a molybdic ion monoclonal antibody marked by colloidal gold is wrapped in the gold label antibody combined cushion. The gold label test paper can realize the rapid detection of molybdenum ion residual contamination in oil, water and food, and has the advantages of being specific, sensitive, rapid, easy and convenient, vivid and visual in result, economic, and the like; the gold label test paper can be used for screening of samples in large batch, also can be used for the rapid detection on samples in small batch, and is wide in application range.
Owner:HENAN INST OF SCI & TECH

New application of rapamycin for inhibiting plant early blight

The invention discloses application of sirolimus in inhibition of plant alternaria solani and further discloses a fungicide. The fungicide contains rapamycin or a mixture of the rapamycin and a chemical fungicide. A TOR protein inhibitor rapamycin used in clinical medicine is used for prevention and control research of plant early blight for the first time, it is found through the research that the rapamycin and the mixture of the rapamycin and the chemical fungicide can effectively inhibit the plant alternaria solani, a chemical rapamycin pesticide can play a synergistic effect to inhibit the plant alternaria solani, a very significative base is provided for research of a novel drug for preventing and controlling the early blight, the novel fungicide is wide in application prospect and market prospect, a new choice is provided for early blight prevention and control, the using amount of the chemical pesticide can be remarkably decreased, and the fungicide has the great significance on reduction of pesticide usage amount and delay of the resistance of insect pests and also has the great application value on maintenance of ecosystem balance and sustainable development.
Owner:CHONGQING UNIV

Fusarium oxysporum tor gene RNAi carrier and method for combined control of potato dry rot and wilt with salicylic acid

The invention discloses a Fusarium oxysporum TOR gene RNAi vector, which uses the plant expression vector pEarleyGate 303 as a backbone vector and inserts the sequence shown in Seq ID No: 3 at the recombination site. A microbial transformant containing the aforementioned RNAi vector is also disclosed. It also discloses the application of the aforementioned RNAi vector in preventing and treating potato dry rot and fusarium wilt. Also disclosed is a method for combined prevention and treatment of potato dry rot and wilt: the aforementioned RNAi vector is transferred into potatoes to obtain transgenic potatoes, and the transgenic potatoes are treated with salicylic acid to prevent dry rot and wilt. The present invention successfully constructs the Fusarium oxysporum RNAi interference vector, and the combined use of the plant hormone salicylic acid and the FoTOR12-RNAi transgene can significantly weaken the pathogenicity of Fusarium oxysporum. disease and dry rot provides an effective method.
Owner:INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI +1

Use of nimbin in preparing seed coating agent

The present invention relates to the application of azadirachtin in the preparation of seed coatings. The application of azadirachtin in seed coatings for the first time overcomes its photosensitivity weakness and is applied to soil, and its insecticidal effect is not washed by light, evaporation and rain. The influence of azadirachtin can protect the maximum amount of drug on the target body, thereby enhancing the stability of azadirachtin, prolonging the effective period, giving full play to the insecticidal and sterilizing functions of azadirachtin, and controlling various seed pests and diseases. At the same time, for the first time, sulfur powder is used as the active ingredient of the seed coating agent to give full play to its protective bactericidal and insecticidal properties and reduce the cost of the seed coating agent. In particular, it has a high co-toxicity coefficient with azadirachtin, which solves the defects that sulfur is easy to precipitate and agglomerate, and also ensures that azadirachtin has better stability.
Owner:重庆荣达建设(集团)有限公司

Marine hydrate exploitation and reinjection system and method

The invention relates to a marine hydrate exploitation and reinjection system and method.The marine hydrate exploitation and reinjection system comprises at least one production well, a gas-liquid separator, at least one reinjection well, a seabed production pipe, a natural gas conveying pipe and a seabed reinjection pipe, and the production well is communicated with a gas-liquid mixture inlet of the gas-liquid separator through the seabed production pipe; a liquid outlet of the gas-liquid separator is communicated with the reinjection well through a seabed reinjection pipe, and a gas outlet of the gas-liquid separator is communicated with the offshore production platform through a natural gas conveying pipe. According to the marine hydrate exploitation and reinjection system and method disclosed by the invention, in the process of exploiting the marine hydrate of the natural gas hydrate reservoir through the production well, the marine hydrate is subjected to gas-liquid separation through the gas-liquid separator, gas is conveyed to the offshore production platform, and meanwhile, liquid is reinjected into the natural gas hydrate reservoir through the reinjection well; further decomposition of marine hydrates in a natural gas hydrate reservoir can be promoted, and the crude oil recovery rate is increased.
Owner:CHINA UNIV OF PETROLEUM (BEIJING) +1
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