30results about How to "Maintain sustainable development" patented technology

The invention relates to an indirect competitive ELISA kit for detecting mercury ions and a preparation method thereof. The kit contains an ELISA plate coated with mercury ion-coated antigen, anti-mercury ion monoclonal antibody, enzyme-labeled secondary antibody, substrate chromogenic solution, stop solution, mercury ion standard solution, washing liquid concentrate and sample processing liquid. The kit of the present invention can detect mercury ions in trace amounts, and is used for detecting mercury ions in the environment, soil, water, food, medicine, cosmetics, etc., and has the characteristics of fast, simple, sensitive, specific and economical, and has few detection steps, saving The detection time is short, the operation error is reduced, the timeliness is strong, and on-site detection can be carried out. The kit has low requirements on sample pre-treatment and simple processing process. It can be used not only for the screening of large batches of samples, but also for the rapid detection of small batches of samples. , Food inspection, environmental pollution monitoring and evaluation, etc. provide effective technical means.

The invention relates to an enzyme linked immunosorbent assay (ELISA) kit for detecting hexavalent molybdenum ions as well as establishment and detection methods thereof. The kit is internally provided with an ELISA plate covered by a hexavalent molybdenum ion coating antigen, a hexavalent molybdenum ion resisting monoclonal anti-antibody, an enzyme-labeled second antibody, a primer developing solution, a stopping solution, a hexavalent molybdenum ion standard solution, a concentrated washing solution and a sample treating solution. Before the detection, 0.1mol / L EDTA (Ethylene Diamine Tetraacetic Acid) chelating agent is used for treating a sample, the hexavalent molybdenum ions and the EDTA are sufficiently chelated to obtain a sample solution to be detected, and then the solution is detected. The kit provided by the invention can detect the trace hexavalent molybdenum ions and the detection sensitivity is 1ng / mL; detection steps are few so that the time is saved, the operation error is reduced and the detection cost is not more than 1 / 20 of that of a physical and chemical analysis method; the timeliness is strong and field detection can be carried out; the kit has the properties of rapidness, simplicity, convenience, sensitivity, specificity, economical efficiency and the like, and is mainly used for sieving large-batch samples polluted by residual molybdenum ions in the environment, soil, water and foods.

The invention relates to an indirect competitive ELISA kit for detecting lead ions and a preparation method thereof. The box body is provided with an enzyme-labeled plate coated with an antigen coated with lead ions, a lead ion monoclonal antibody, an enzyme-labeled secondary antibody, Substrate chromogenic solution, stop solution, lead ion standard solution, washing solution concentrate and sample treatment solution. The lead ion indirect competitive ELISA kit of the present invention can detect lead ions in trace amounts, can be used for the detection of lead ion pollution residues in the environment, soil, water, food and utensils, and has the advantages of fast, simple, sensitive, specific and economical and other characteristics, less detection steps, saving detection time, reducing operational errors; time-sensitive, can be used for on-site detection; it can be used not only for the screening of large batches of samples, but also for the rapid detection of small batches of samples. , Environmental pollution monitoring and evaluation, etc. provide effective means.

The invention discloses an RNAi (ribonucleic acid interference) expression vector for TOR (target of rapamycin) genes of plutella xylostella, a method for constructing the RNAi expression vector and application thereof. The RNAi expression vector comprises separate polynucleotide, a vector and a recombinant vector. Sequences of the separate polynucleotide are shown as SEQ ID NO:1 and SEQ ID NO:2; the vector comprises the polynucleotide with the sequences shown as SEQ ID NO:1 or SEQ ID NO:2; the recombinant vector comprises sequences shown as SEQ ID NO:7, and pEarleyGate 303 is used as a skeleton of the RNAi expression vector for the TOR genes of the plutella xylostella. The method for preparing the RNAi expression vector includes displacing and inserting EcoR I, AsiS I, P35S promoters, Not I, the sequences SEQ ID NO:1, Sbf I, fourth introns of ACTIN, BbvC I, reverse complement sequences of the sequences SEQ ID NO:1, Asc I, T35S terminators and EcoR I, which are sequentially connected with one another, in recombination sites.

The invention discloses a secondary treatment method of a flue gas desulfurization liquid treated with a sodium method. The secondary treatment method comprises operation steps as follows: the sodium-method desulfurization liquid is sent into a sulfur burning furnace to be burnt, and produced high-temperature gas and little sulfate are rapidly cooled by a quench cooler; the cooled gas and sulfate are mixed in the quench cooler and then enter a gas-liquid separation tank, a sulfate solution and gas are formed after vapor is condensed, gas obtained after gas-liquid separation is sucked away by a fan, the sulfate solution is treated by a sulfate solution cooler, passes by the quench cooler and returns to the gas-liquid separation tank, and the operation is cycled until the sulfate solution is saturated; the saturated sulfate solution is concentrated and crystalized by a sulfate multi-effect evaporation device or an MVR (mechanical vapor recompression) evaporation device, and waste recycling is realized. The secondary treatment method adopts reasonable process procedure design, has the advantages that the sewage treatment effect is good, the recycling rate is high, the operation is simple and convenient, system operation is stable and blocking is difficult to cause, and can be widely applied to factory treatment of the flue gas desulfurization liquid treated with the sodium method.

The invention discloses a brown planthopper sialaden secretor gene N1MLP and an application OF brown planthopper NIMLP in paddy resisting against brown planthopper after the gene N1MLP is restrained. A complete cDNA sequence of the brown planthopper sialaden secretor gene N1MLP is shown as SEQ ID No.1; ds RNA of the N1MLP enters the brown planthopper in the manner of micro-injection, so that the N1MLP gene expression quantity is reduced, the feeding of the brown planthopper is affected and the survival rate of the brown planthopper is reduced; the ds RNA of the N1MLP is stably expressed in the paddy plant; after the brown planthopper eats the paddy, the N1MLP gene expression quantity is reduced, so that the brown planthopper weight is reduced and the survival rate of the brown planthopper is reduced. According to the invention, the N1MLP, of which the expression is restrained, has significance in researching the paddy resisting against brown planthopper and preventing and treating the brown planthopper in agricultural production.

The invention discloses a glaze material manufactured by utilization of tailings. The glaze material comprises 40-65% of feldspar, 10-25% of quartz, 15-30% of iron ore mine tailings, 0-10% of limestone, 0-10% of kaolin and 0-10% of bone ash. The glaze material comprises following components by weight: 1-5 parts of K2O, 1-8 parts of Al2O3, 55-70 parts of SiO2, 1-5 parts of P2O5, 1-5 parts of Na2O, 1-8 parts of Fe2O3, 5-15 parts of CaO and 1-8 parts of MgO. The invention also discloses a glazing process of the glaze material manufactured by utilization of the tailings. The glazing process includes: adding the materials into a ball grinder and performing ball-milling, adding water, uniformly mixing, stirring and dissolving to obtain glaze slurry, with the fineness of the glaze slurry being controlled to be less than 0.01% of residue on sieve of a ten-thousand hole sieve; discharging the glaze slurry and stirring; sieving the glaze slurry with a sieve having a size of 200 meshes, applying the fully suspended glaze slurry onto the surface of a green body with the concentration being 38-46 baume degrees and the thickness of the glaze slurry on the surface of the green body being 0.2-1.2 mm; and subjecting the green body to high-temperature sintering, and cooling in furnace to room temperature after the sintering is finished to obtain a ceramic product. By adoption of a one-time sintering process, the product quality can reach that of secondary sintering and the product energy consumption is reduced.

The invention discloses application of a TOR protein inhibitor to inhibition of wilt fusarium and verticillium dahliae of plants and a fungus bactericide. The fungus bactericide contains the TOR protein inhibitor, wherein the TOR protein inhibitor is Rapamycin, Torin 1, a mixture of Rapamycin and Torin 1, or a mixture of Rapamycin and a fungus chemical bactericide. According to the application disclosed by the invention, the TOR protein inhibitor used for clinical medicine is used for prevention and treatment researches of the wilt fusarium and the verticillium dahliae of the plants for the first time; researches show that the TOR protein inhibitor including Rapamycin, Torin 1, the mixture of Rapamycin and Torin 1 and the mixture of Rapamycin and the fungus chemical bactericide can be used for effectively inhibiting the wilt fusarium and the verticillium dahliae of the plants, so that a new choice for preventing and treating wilt and verticillium wilt is provided, and has very important meanings on reducing the utilization of pesticides and delaying the insect resistance.

The invention discloses gold label test paper for rapidly detecting molybdic ions, as well as a preparation method and an application thereof. The bottom layer of the gold label test paper is a supporting layer, the middle layer of the gold label test paper is an adsorption layer, a protective film is fixedly arranged on the absorption layer, the absorption layer comprises a sample cushion, a gold label antibody combined cushion, a cellulose film layer and a handle-end absorbent cushion from a test end in sequence, and detection imprints printed by a carrier protein solution of coupling molybdic ions and contrast imprints printed by a rabbit anti-mouse IgG antibody solution are arranged on the absorbent cushion; a molybdic ion monoclonal antibody marked by colloidal gold is wrapped in the gold label antibody combined cushion. The gold label test paper can realize the rapid detection of molybdenum ion residual contamination in oil, water and food, and has the advantages of being specific, sensitive, rapid, easy and convenient, vivid and visual in result, economic, and the like; the gold label test paper can be used for screening of samples in large batch, also can be used for the rapid detection on samples in small batch, and is wide in application range.

The invention discloses a Fusarium oxysporum TOR gene RNAi vector, which uses the plant expression vector pEarleyGate 303 as a backbone vector and inserts the sequence shown in Seq ID No: 3 at the recombination site. A microbial transformant containing the aforementioned RNAi vector is also disclosed. It also discloses the application of the aforementioned RNAi vector in preventing and treating potato dry rot and fusarium wilt. Also disclosed is a method for combined prevention and treatment of potato dry rot and wilt: the aforementioned RNAi vector is transferred into potatoes to obtain transgenic potatoes, and the transgenic potatoes are treated with salicylic acid to prevent dry rot and wilt. The present invention successfully constructs the Fusarium oxysporum RNAi interference vector, and the combined use of the plant hormone salicylic acid and the FoTOR12-RNAi transgene can significantly weaken the pathogenicity of Fusarium oxysporum. disease and dry rot provides an effective method.

The present invention relates to the application of azadirachtin in the preparation of seed coatings. The application of azadirachtin in seed coatings for the first time overcomes its photosensitivity weakness and is applied to soil, and its insecticidal effect is not washed by light, evaporation and rain. The influence of azadirachtin can protect the maximum amount of drug on the target body, thereby enhancing the stability of azadirachtin, prolonging the effective period, giving full play to the insecticidal and sterilizing functions of azadirachtin, and controlling various seed pests and diseases. At the same time, for the first time, sulfur powder is used as the active ingredient of the seed coating agent to give full play to its protective bactericidal and insecticidal properties and reduce the cost of the seed coating agent. In particular, it has a high co-toxicity coefficient with azadirachtin, which solves the defects that sulfur is easy to precipitate and agglomerate, and also ensures that azadirachtin has better stability.