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Application of glutathione waste liquid in microorganism production and application method

A technology of glutathione and application method, applied in the field of microorganisms, can solve the problems of high capital construction investment and operation and maintenance costs, low utilization rate of waste recycling, unreported research results, etc., and achieve huge social and economic benefits. The effect of improving the quality of agricultural products and improving soil fertility

Inactive Publication Date: 2015-11-25
JINAN YIBANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Direct discharge of various waste liquids will not only seriously pollute the environment, but also cause waste of resources
Using traditional physicochemical treatment technology and biological treatment technology such as coagulation method, evaporation concentration method, adsorption method, activated sludge method, biological activated carbon method, biofilm method, etc. to treat wastewater, although it can meet the standard, the capital investment and operation and maintenance costs are high. , the process is complicated, the recycling rate of waste is low, and it is easy to produce secondary pollution
[0006] The recycling of glutathione waste liquid is the best strategy for the sustainable development of glutathione enterprises, but the relevant research results have not been reported yet

Method used

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  • Application of glutathione waste liquid in microorganism production and application method
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  • Application of glutathione waste liquid in microorganism production and application method

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0046] 【raw material】

[0047] In September 2014, the glutathione waste liquid (that is, the centrifuged residue of the fermentation liquid produced during the production of glutathione by microbial fermentation) from Shandong Jincheng Bio-Pharmaceutical Co., Ltd. was used as raw material. Tested by the Institute of Agricultural Resources and Environment of Shandong Academy of Agricultural Sciences, its composition is: nitrogen 1.51g / 100mL; phosphorus 0.10g / 100mL; potassium 3.05g / 100mL; organic matter 40.75g / 100mL; soluble solid content 38.4g / 100mL, Sugar content 18g / 100mL, pH 5.11; moisture 54.34%.

[0048] After testing by the calendar L-8900 high-speed automatic amino acid analyzer of the Urban Agriculture (North) Key Laboratory of the Ministry of Agriculture, the analysis of the 18 kinds of amino acids and crude protein content is shown in Table 1:

[0049] Analysis of 18 kinds of amino acids and crude protein content in glutathione waste liquid in table 1

[0050]

...

preparation Embodiment 2

[0069] 【raw material】

[0070] Same as Preparation Example 1.

[0071] 【Source of bacteria】

[0072] Lactobacillus acidophilus (provided in the form: ampule freeze-dried powder) was purchased from China Industrial Microorganism Culture Collection Management Center, number CICC6074).

[0073] 【Preparation of Fermentation Broth】

[0074] 1) Activation of Lactobacillus acidophilus

[0075] Slant medium preparation: 1% casein peptone, 1% beef extract, 0.5% yeast powder, 0.5% glucose, 0.5% sodium acetate, 0.2% diammonium citrate, Tween800.1%, K 2 HPO 4 0.2%, MgSO 4 ·7H 2 O0.02%, CaCO 3 2%, agar 1.5%, adjust the pH of the medium to 5.8, 121°C, 0.103MPa, sterilize for 30min, and prepare inclined test tubes under sterile conditions.

[0076] Activation method: under sterile conditions, inoculate the slant with Lactobacillus acidophilus in the cryopreservation tube, and activate it at 36°C for 36 hours to obtain the slant strain or strain slant.

[0077] 2) Preparation of Lact...

preparation Embodiment 3

[0088] 【raw material】

[0089] In December 2014, the glutathione waste liquid (that is, the centrifuged residue of the fermentation liquid produced during the production of glutathione by microbial fermentation) from Shandong Jincheng Bio-Pharmaceutical Co., Ltd. was used as raw material. Tested by the Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Sciences, its composition: nitrogen 1.53g / 100mL; phosphorus 0.097g / 100mL; potassium 3.04g / 100mL; organic matter 40.97g / 100mL; soluble solid content 38.33g / 100mL, Sugar content 17.78g / 100mL, pH 5.22; moisture 53.94%.

[0090] After testing by the calendar L-8900 high-speed automatic amino acid analyzer of the Urban Agriculture (Northern) Key Laboratory of the Ministry of Agriculture, the analysis of the 18 kinds of amino acids and crude protein content is shown in Table 2:

[0091] 18 kinds of amino acids and crude protein content analysis in table 2 glutathione waste liquid

[0092]

[009...

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Abstract

The invention discloses application of glutathione waste liquid in microorganism production and an application method and belongs to the field of microorganisms. The glutathione waste liquid is used as the effective constituent of a seeding tank culture medium and / or a fermentation tank culture medium to be applied to the microorganism production, good social and economic values are achieved, microorganism preparation with good performance can be obtained, and wide application value is achieved.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to the application and application method of glutathione waste liquid in microorganism production. Background technique [0002] Glutathione, generally refers to reduced glutathione (Glutathione, GSH), which is a biologically active substance widely present in normal cells of the human body, and is the most abundant sulfhydryl-containing low-lying compound naturally synthesized in human cells. Molecular tripeptide, clinically proven that reduced glutathione is a widely used small molecule peptide active substance, which has important anti-oxidation and integrated detoxification effects, and is used for viral, drug toxicity, alcohol toxicity and other chemical substances. It is also an adjuvant therapy drug for cancer patients during radiotherapy and chemotherapy, and has a positive effect on the combined treatment of acute anemia, adult respiratory distress syndrome, and sepsis. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12R1/10C12R1/23C12R1/125C12R1/72C12R1/07
Inventor 常钟钱朋智史晓洁蔡洋赵传涛侯大彬
Owner JINAN YIBANG BIOTECH CO LTD
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