47results about How to "Low bacteria rate" patented technology

The invention relates to the biotechnical field, and in particular relates to a method for producing bacillus laterosporus live bacteria by high density solid fermentation. The method comprises the following steps: culture selection; bevel seed cultivation; primary solid seed cultivation; secondary solid seed cultivation; solid fermentation and cultivation; fermentation product drying; and fermentation product flash evaporation. Raw materials of the culture media provided by the invention are subsidiary agricultural products which are low in cost and reasonable in formula and can meet the nutritional demand of strain reproduction. The pH value of the culture media in the process of cultivation by adding a calcium carbonate buffer is in particularly changed, so that the content of active bacteria of bacillus laterosporus by solid fermentation is greatly improved. Experiments and production show that the number of effective active bacteria of bacillus laterosporus prepared by the method can reach 50 billion / g, the percentage of contaminating microorganisms of the product is lower than 5% and the spore rate reaches over 90%, so that the production coast of enterprises is greatly lowered, and the demand of production of microbial agents is satisfied.

The invention provides a streptomyces microflavus solid fermentation medium and a preparation method thereof, a streptomyces microflavus fermentation method and fermentation product, bio-control products of the fermentation product as well as application of the fermentation product and bio-control products, and relates to the technical field of microbial fermentation. The streptomyces microflavussolid fermentation medium is prepared from the following components in parts by weight: 40-50 parts of bran, 15-25 parts of rice hull, 25-35 pats of rice bran, 3-7 parts of calcium carbonate, and water. The streptomyces microflavus fermentation method comprises the steps of optionally enabling a strain of streptomyces microflavus to be subjected to seed activation and liquid seed culture, and performing fermentation culture by using the streptomyces microflavus solid fermentation medium to obtain the fermentation product. The streptomyces microflavus fermentation method overcomes the defect that the existing streptomyces microflavus is less in viable count and sporulation quantity. When being used for solid fermentation culture, the streptomyces microflavus solid fermentation medium is short in fermentation cycle and more in viable count, thus being suitable for industrial production; the obtained fermentation product has a significant effect in the aspect of controlling bacterial wiltof crops, thus increasing the quality and yield of the crops.

The invention provides a method for preparing trichoderma harzianum high-activity conidial powder. The method comprises the following steps: performing step-by-step propagating fermentation on a trichoderma harzianum strain, from preparation production of stock culture, mother spawn and spawn to production of high-concentration trichoderma harzianum chlamydospore fermentation liquor by adopting astepwise liquid fermentation method; and concentrating the fermentation liquor, adding different functional additives and filler into the concentrated fermentation liquor, and performing drying treatment to obtain the trichoderma harzianum high-activity conidial powder. The trichoderma harzianum chlamydospore has the number of effective living bacteria being 2*10<9>cfu / g or more, and has the advantages of high activity, long shelf life, high germination rate, quick soil environment adaptability and the like.

The invention discloses a culture method of selenium-rich black fungus, and relates to the technical field of black fungus planting. According to the culture method of the selenium-rich black fungus, hexamethylphosphoramide is added into a culture medium and is combined with sodium selenite in the culture medium to generate organic selenium; sodium selenite inorganic selenium is converted into organic selenium, so that the absorption of the black fungus on the selenium element can be promoted; the goal of enriching the selenium enrichment by the black fungus is achieved; traditional Chinese medicine ingredients are also added in the culture medium; the effects of sterilization, mildew prevention, worm expelling, black fungus immunity improvement, growth promotion and yield increase are achieved; the normal growth of the black fungus is ensured; the survival rate is improved; the traditional Chinese medicine ingredients are plant sources, are easy to decompose, so that the pesticide residue cannot be caused; scientificalness and environment protection are realized; the health of eaters can be ensured.

The invention relates to a preparation method of active lactic acid bacteria pickles, belonging to the technical field of biology and food. The process steps of the present invention include: A. kimchi water filling altar, B. vegetable filling, C. constant temperature fermentation at 12-18°C, D. dispensing and packaging out of the altar; The kimchi water in the altar is made by adding the direct-injection type lactic acid bacteria starter and the direct-injection type lactic acid bacteria starter activation solution made from glucose, and after mixing evenly, the second round of circulation kimchi production is carried out. The present invention adopts a reasonable vegetable ratio and good-flavored capsicum-vegetable fermented pickling water, which better maintains the inherent and fermented flavor of the vegetable, realizes the standardized production of active lactic acid bacteria pickles, and improves the quality and uniformity of the product , significantly improved the biological safety of the product, achieved the best flavor and the most suitable taste, and then realized the upgrading of traditional active lactic acid bacteria kimchi and industrialized, large-scale and standardized production.

The invention discloses a method for recycling municipal sludge. The method comprises mixing sludge, straws, YM bacteria, an amino acid stock solution, bacillus amyloliquefaciens SQR9 functional bacteria and trichoderma harzianum T37 functional fungi, conducting high temperature and low oxygen fermentation, conducting crushing, sieving and functional fermentation, and then crushing and sieving toobtain a finished product. Main raw materials are the municipal sludge and the crop straws that are large in quantity, large-scale and periodic production can be achieved, the sludge is from a local sewage treatment plant and meets the CJ / T309-2009 standard, and a raw material source is safe. The high temperature low oxygen and functional fermentation technology is utilized to solve the problem ofenvironment pollution caused by organic solid waste and achieve organic solid waste resource utilization and stable utilization.

The invention provides a fermental cultivation process of bacillus licheniformis. The fermental cultivation process comprises the seed tank cultivation stage and the fermentation tank cultivation stage, specifically, the liquefying, sterilizing, inoculating and cultivating steps are included at the seed tank cultivation stage, and culture medium raw materials adopted for seed tank cultivation comprise corn flour, soybean meal, glucose, dipotassium phosphate, monopotassium phosphate, magnesium sulfate and manganese sulfate; and during fermental cultivation, the ventilation quantity in the firstfour hours is 1:(1 to 1.1), the later ventilation quantity is 1:(1.2 to 1.3), the cultivation temperature is 37+ / -0.5 DEG C, the stirring speed is 150-160 rpm in the first four hours and is 180-190 rpm after four hours, and the cultivation time is 30+ / -0.5 hours. According to the fermental cultivation process of the bacillus licheniformis, the gemma rate of the bacillus licheniformis reaches 98%or above.

The invention relates to a microbial bacterial agent, which contains Trichoderma aculeatus WL, live spores of Bacillus stearothermophilus and Claudium acremonium. Composting organic garbage by using the compound microbial bacterial agent is beneficial to improving the maturity of materials, and has a complexing effect on heavy metal ions to change the state of heavy metal ions and has strong tolerance. The present invention also relates to a sandy soil remediation agent for organic waste, which is obtained by mixing fermentation products and organic waste preparations; Obtained; the preparation method of the organic waste preparation comprises: the organic waste is soaked in water for 10-14 hours after removing impurities and crushing, stirring into a paste under the environment of pH 9-10, and then adding vinyl monomers to carry out graft copolymerization reaction . The soil remediation agent solves the problem of land desertification and remediates soil polluted by heavy metals.

The invention discloses a culture medium special for Fritillaria thunbergii and a preparation process and system of the culture medium. The culture medium and the preparation process and system have the advantages that bamboo carbon and refined bamboo vinegar serving as the main raw materials are compounded to obtain the culture medium special for the Fritillaria thunbergii, and the culture mediumcan be directly applied to the seedling culture of the Fritillaria thunbergii and can also be directly applied in the field to serve as the medium accompanying fertilizer; by the culture medium, maximized compounding of nutrition needed by the propagation of Fritillaria thunbergii bulbs is achieved, maximized utilization of key nutrition is guaranteed, and Fritillaria thunbergii vegetative propagation emergence rate can be increased.

The invention provides a compounded amino-acid microbial bacterium agent and a preparation method therefor. The microbial bacterium agent is prepared from the raw material ingredients in parts by weight: 38 parts of Sphingomonas yanoikuyae powder, 12 parts of Lactobacillus bulgaricus powder, 25 parts of Azotobacter chroococcum powder, 20 parts of Bacillus laterosporus powder, 9 parts of compounded amino acid salt, 20 parts of zymosan and 26 parts of choline chloride. The compounded amino acid salt contains L-histidine salt, L-cysteine salt, L-ornithine salt and L-lysine-L-aspartate, wherein the weight content ratio of the L-histidine salt to the L-cysteine salt to the L-ornithine salt to the L-lysine-L-aspartate is 5: 3: 2: 2. The compounded amino-acid microbial bacterium agent provided by the invention has the pH value of 6.8 and the moisture content of 18-22%; the content of effective living bacteria is 6.6-7.9*10<9> / g, and the ratio of miscellaneous bacteria is 3.2-4.7%.

The invention relates to the field of biotechnology, in particular to a method for producing viable bacillus licheniformis by high-density solid fermentation, which comprises the following steps: strain selection, slant seed cultivation, solid seed cultivation, solid fermentation cultivation, fermentation product drying and The fermentation product is flash evaporated. The raw materials of the culture medium of the present invention are all agricultural by-products, the cost is low, the formula is reasonable, and the nutritional requirements for the propagation of bacteria can be met. Especially adding the pH value change of medium in the calcium carbonate buffer culture process can greatly improve the effective viable bacteria content of bacillus licheniformis of solid fermentation, and experiment and production prove that the effective viable bacteria number of bacillus licheniformis prepared by the inventive method can reach Up to 100 billion / g, the rate of miscellaneous bacteria in the product is less than 5%, and the rate of spores is high, reaching more than 90%, which can greatly reduce the production cost of enterprises and meet the needs of microbial bacterial agent production.