Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Trichoderma viride solid fermentation technology

A solid fermentation, Trichoderma viride technology, applied in fungi, microorganisms, microorganisms and other directions, can solve the problems of low spore yield, high rate of miscellaneous bacteria, long sporulation time, etc., and achieve low rate of miscellaneous bacteria, high yield and high quality. good stability

Active Publication Date: 2019-11-05
SHANDONG SUKAHAN BIO TECH
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The Trichoderma viride fermentation process of the prior art also has the following defects: the spore yield is low, the sporulation time is long, and the miscellaneous bacteria rate is high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 A kind of Trichoderma viride solid fermentation process

[0024] A Trichoderma viride solid fermentation process comprises a seed tank culture stage and a fermenter tank culture stage.

[0025] First, seed pot culture

[0026] Use 100 liter seed tank; inoculum volume 1000ml

[0027] 1. After the seed tank is checked correctly, put all the raw materials into it, set the volume (50L), heat up to 121-122°C for 30 minutes to sterilize, the volume after disinfection is 70 liters, and the pH after disinfection is controlled at 5.0.

[0028] 2. Vaccination

[0029] After the disinfection is completed, the seed tank is kept under pressure and cooled down, and when the temperature drops to the cultivation temperature of 30±0.5°C, it is ready for inoculation.

[0030] Prepare alcohol cotton, forceps, tweezers and inoculation loop before inoculation. Place the cotton evenly around the inoculation loop, place the inoculation loop at the inoculation port and ignite ...

Embodiment 2

[0054] Embodiment 2 A kind of Trichoderma viride solid fermentation process

[0055] A Trichoderma viride solid fermentation process comprises a seed tank culture stage and a fermenter tank culture stage.

[0056] The strain preservation number of the Trichoderma viride is CICC 13027.

[0057] , seed tank culture

[0058] Use 100 liters of seed tank: inoculum volume 1000ml;

[0059] 1) Seed tank culture medium preparation

[0060] The seed tank medium used is: yeast extract 18g / L, glucose 25g / L, ammonium sulfate 2g / L, shiitake mushroom extract 1.8g / L, malt extract 0.6g / L and cysteine ​​0.2g / L;

[0061] The mushroom extract: purchased from Xi'an Wanfang Biotechnology Co., Ltd., item number WF-5002;

[0062] The malt extract: purchased from Guangzhou Quancao Biotechnology Co., Ltd., item number JG-167.

[0063]After the seed tank is checked correctly, put all the seed tank medium raw materials into it, set the volume to 50L, heat up to 121-122°C for 30 minutes to sterilize,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a trichoderma viride solid fermentation technology. The trichoderma viride solid fermentation technology comprises the processes of seeding tank cultivating and fermentation tank cultivating. According to the seeding tank cultivating process, the pH of a culture medium is controlled to be 5.0-5.2, the culture pressure is 0.05-0.06 Mpa, and the culture temperature is 30+ / -0.5DEG C. According to the fermentation tank cultivating process, the ventilation quantity is 1 to 1, the stirring speed is 180-190 r / s, and the culture time is 23-24 hours. According to the trichodermaviride solid fermentation technology, the yield of spore is high, and the spore number of trichoderma viride after preparation and drying is greater than or equal to 1.5*10<10> piece / g.

Description

technical field [0001] The invention relates to a solid fermentation process of Trichoderma viride, belonging to the technical field of microorganism cultivation. Background technique [0002] Trichoderma viride is a kind of Trichoderma, widely distributed in nature, often saprophytic on wood, seeds and plant residues. Trichoderma viride can produce a variety of enzymes with biological activity, and it is one of the strains with the highest cellulase activity. The cellulase produced can degrade crops, and the effect is very good. Trichoderma viride is another resource Abundant antagonistic microorganisms play an important role in biological control of plant pathology. [0003] CN201710426936.4 discloses a kind of Trichoderma viride and its cultivation method. The Trichoderma viridans strain of the present invention is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No.5612....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00C12R1/885
CPCC12N1/14C12N3/00
Inventor 郭芳先韩威华陈永科刘镇王于玺肖发沂马爱霞
Owner SHANDONG SUKAHAN BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com