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Preparation method and application of microbial agent adopting nanobubbles for activation and expanding culture

A microbial agent and nano-bubble technology, applied in the direction of microbial treatment, application, combined biological treatment, etc., can solve the problems of limited number of colonies, limited effective rate of viable bacteria, high rate of miscellaneous bacteria, achieve safe and reliable effect, improve effective activity Bacteria rate, high purity effect

Inactive Publication Date: 2021-06-11
上海金相环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of the limited number of colonies and limited effective viable bacteria rate in the prior art after the expansion of EM original species; and the biological activity has no obvious advantages compared with the original microbial system in nature, and it is easy to dissimilate and reduce the rate of miscellaneous bacteria after application. In view of the high problem, a preparation method and application of a microbial agent using nanobubble activation and expansion cultivation are proposed

Method used

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  • Preparation method and application of microbial agent adopting nanobubbles for activation and expanding culture
  • Preparation method and application of microbial agent adopting nanobubbles for activation and expanding culture
  • Preparation method and application of microbial agent adopting nanobubbles for activation and expanding culture

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Embodiment 1

[0032] The preparation method of the microbial bacterial agent proposed by the present invention using nano-bubble activation and expansion culture comprises the following steps:

[0033] S1, strain activation:

[0034] S11. Fermentation tank and auxiliary equipment treatment process, the specific operation is to clean and ultraviolet sterilize the required fermentation tank and auxiliary equipment, then heat the water in the fermentation tank for one day, and keep the heating temperature at 35°C;

[0035] S12. Add brown sugar to boiling water at 100°C and boil for 2 minutes, dissolve the brown sugar and cool down to 35°C to make sterile brown sugar water, add the sterile brown sugar water, honey and bacteria liquid to the primary fermentation tank, and stir After uniformity, through the expansion process combined with the use of micro-nano bubble generators for sealed fermentation, the temperature during the expansion process is kept at 35°C to jointly activate the bacteria a...

Embodiment 2

[0042] The preparation method of the microbial bacterial agent proposed by the present invention using nano-bubble activation and expansion culture comprises the following steps:

[0043] S1, strain activation:

[0044] S11. Fermentation tank and auxiliary equipment treatment process, the specific operation is to clean and ultraviolet sterilize the required fermentation tank and auxiliary equipment, then heat the water in the fermentation tank for one day, and keep the heating temperature at 35°C;

[0045] S12. Add brown sugar to boiling water at 100°C and cook for 3 minutes, dissolve the brown sugar and cool down to 35°C to prepare sterile brown sugar water, add sterile brown sugar water, honey and bacteria liquid to the primary fermentation tank, and stir After uniformity, through the expansion process combined with the use of micro-nano bubble generators for sealed fermentation, the temperature during the expansion process is kept at 35°C to jointly activate the bacteria an...

Embodiment 3

[0052] The preparation method of the microbial bacterial agent proposed by the present invention using nano-bubble activation and expansion culture comprises the following steps:

[0053] S1, strain activation:

[0054] S11. Fermentation tank and auxiliary equipment treatment process, the specific operation is to clean and ultraviolet sterilize the required fermentation tank and auxiliary equipment, then heat the water in the fermentation tank for one day, and keep the heating temperature at 35°C;

[0055]S12. Add brown sugar to boiling water at 100°C and boil for 2 minutes, dissolve the brown sugar and cool down to 35°C to make sterile brown sugar water, add the sterile brown sugar water, honey and bacteria liquid to the primary fermentation tank, and stir After uniformity, through the expansion process combined with the use of micro-nano bubble generators for sealed fermentation, the temperature during the expansion process is kept at 35°C to jointly activate the bacteria an...

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Abstract

The invention belongs to the technical field of preparation methods of microbial agents, particularly relates to a preparation method and application of a microbial agent adopting nano-bubble for activation and expanding culture, and solves the problem that in the prior art, after expanding culture of EM stock seeds, the number of colonies is limited, and the effective viable bacterium rate is limited and the problem that compared with the original microbial system in nature, there is no obvious advantage on bioactivity of the microbial system, and easy dissimilation and high infectious microbe rate are caused after application. The preparation method of the microbial agent adopting nano-bubbles for activation and expanding culture includes the following steps that sterile brown sugar water, honey and strain liquid are jointly added into a first-stage fermentation tank, and fermentation is carried out through combination of an expanding culture process and a micro-nano bubble generation device; and the activated strain liquid, the sterile water and the sterile brown sugar water are jointly poured into a secondary fermentation tank, and fermentation is carried out through combination of an expanding culture process and a micro-nano bubble generation device. The effective viable bacterium rate of the EM stock compound bacterial colony group is greatly improved, the purity is higher, and the infectious microbe rate is reduced to 0.1% or below.

Description

technical field [0001] The invention relates to the technical field of preparation methods of microbial bacterial agents, in particular to a preparation method and application of microbial bacterial agents activated and expanded by nano-bubbles. Background technique [0002] EM is the abbreviation of English (Effective Microorganisms), translated into Chinese as: "Effective Microorganisms". It is composed of more than 80 species of microorganisms in 5 families, 10 genera, including photosynthetic bacteria, lactic acid bacteria, yeast flora, actinomycetes, and filamentous flora. of. EM technology is currently the most widely used bioengineering technology in the world. Compared with general biological agents, it has the advantages of complex structure, stable performance and complete functions, showing an unprecedented high-tech level. According to relevant reports, EM is widely used in various fields such as planting, breeding, environmental protection, and human health ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C05F17/20C05F17/50C05F11/08C05G3/80C05G5/20
CPCC12N1/20C12N1/16C05F17/20C05F17/50C05F11/08C05G3/80C05G5/20Y02W30/40
Inventor 陈鲁海陈邦林王国平王国祥
Owner 上海金相环境科技有限公司
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