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Solid culture medium used for lactobacillus plantarum fermentation, lactobacillus plantarum solid inoculant, and preparation method for and application of lactobacillus plantarum solid inoculant

A solid culture medium, Lactobacillus plantarum technology, applied in microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problem of high variation coefficient of mixing uniformity, low effective viable bacteria count of inoculants, complex operation procedures, etc. problems, to achieve the effect of high effective viable bacteria, small coefficient of variation, and simplified preparation process

Active Publication Date: 2021-01-05
COFCO BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the preparation of solid bacterial agents generally includes steps such as preparing seed liquid, collecting bacteria, adding carriers, and drying. Susceptible to bacteria and other problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] The second aspect of the present invention provides a method for preparing a solid bacterial agent of Lactobacillus plantarum, the method comprising: inoculating Lactobacillus plantarum strains into the above-mentioned solid medium for fermentation.

[0026] In the present invention, the activated Lactobacillus plantarum can be inoculated into the above-mentioned solid medium by conventional technical means in the art. Preferably, the inoculation method may include: mixing the seed liquid of Lactobacillus plantarum with water to obtain an inoculation liquid, and then uniformly mixing the inoculum liquid with the solid medium to obtain a fermentation starting material. Preferably, the inoculum is mixed with the solid medium by spraying.

[0027] In the present invention, relative to every gram of solid medium, the inoculation amount of the plantarum lactobacillus can be selected in a wide range, preferably 1×10 5 -1×10 9 cfu, more preferably 1×10 6 -1×10 8 cfu.

[0...

Embodiment

[0042] The following describes this article in detail in conjunction with specific embodiments. The advantages and features of the present invention will become clearer with the description. However, these embodiments are only exemplary and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention.

[0043] The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents and equipment used in the following examples can be obtained from commercial sources unless otherwise specified.

[0044] In the following examples and comparative examples:

[0045] Lactobacillus plantarum (L...

preparation example

[0055] This preparation example is used to prepare the Lactobacillus plantarum seed liquid used in the following examples

[0056] Sterilize the above primary seed medium at 121°C for 20 minutes, cool to room temperature; inoculate 200ml of the above primary seed medium with 0.5% by volume of Lactobacillus plantarum from the cryopreservation tube, and culture at 35°C After 15 hours, the primary seed solution was obtained. The number of colonies was 10 billion cfu / ml.

[0057] Sterilize the above-mentioned secondary seed medium at 121°C for 20 minutes, cool to room temperature; inoculate 2% by volume of bacterial liquid from the primary seed liquid into the secondary seed medium, stir once every 5h, rotate at 10rpm, 35°C Cultivate for 18 hours to obtain secondary seed liquid. The number of colonies was 20 billion cfu / ml.

[0058] The expansion medium was sterilized at 121°C for 20 minutes, and cooled to room temperature; 5% by volume of the bacterial liquid was drawn from th...

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Abstract

The invention relates to the field of microbial inoculants, and discloses a solid culture medium used for lactobacillus plantarum fermentation, a lactobacillus plantarum solid inoculant, and a preparation method for and application of the lactobacillus plantarum solid inoculant. The solid culture medium used for lactobacillus plantarum fermentation is prepared from the following raw materials: a starchy raw material crushed product, starch dregs, carbonate, yeast extract powder and saccharides. The invention further provides a preparation method for the lactobacillus plantarum solid inoculant,and the method comprises the step of inoculating a lactobacillus plantarum strain into the solid culture medium for fermentation. The lactobacillus plantarum solid inoculant is produced through a solid state fermentation method by using the solid culture medium disclosed by the invention, so that the prepared lactobacillus plantarum solid inoculant has full and strong thalli, the effective viablecount is greater than 500 hundred million cfu / g, and the mixing uniformity variable coefficient is less than 5%; The lactobacillus plantarum solid inoculant has few operation steps and low mixed bacteria rate; the product quality is promoted; equipment and energy consumption is reduced, and wastewater is greatly reduced, thereby reducing the environmental burden.

Description

technical field [0001] The invention relates to the field of microbial inoculum, in particular to a solid culture medium for fermentation of Lactobacillus plantarum, a solid Lactobacillus planta inoculum and a preparation method and application thereof. Background technique [0002] With the popularization of the application range of fermented feed, it is imminent to increase the production of fermented feed. However, due to the complex preparation process and short storage period of liquid inoculum, the preparation of liquid inoculum has become a key factor restricting the production of fermented feed. The solid microbial agent has a long storage period, is convenient for transportation and storage, can solve the limitations of production equipment, technology and manpower, and becomes an important factor for stabilizing and improving product quality and increasing production. Therefore, the application of solid microbial agent to fermented feed is a breakthrough The develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23K10/18C12R1/25
CPCC12N1/20C12N1/04A23K10/18Y02P60/87
Inventor 佟毅卢宗梅张德国陈影郑晓卫焦琳
Owner COFCO BIOTECHNOLOGY CO LTD
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