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Fermental cultivation process of bacillus licheniformis

A technology of Bacillus licheniformis and fermentation culture, applied in the directions of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of inconsistent spore formation, unstable spore quantity and spore quality, and low fermentation level, and achieve long and stable shelf life. The effect of good performance and low bacterial rate

Active Publication Date: 2019-10-08
SHANDONG SUKAHAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the fermentation process of Bacillus licheniformis generally has defects such as low fermentation level, inconsistent spore formation, unstable spore quantity and spore quality, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A Bacillus licheniformis fermentation culture process comprises a seed tank culture stage and a fermenter tank culture stage.

[0038] The strain used was Bacillus licheniformis ACCC10146.

[0039] First, seed pot culture

[0040] Use 100 liters of seed tank: inoculum volume 100ml;

[0041] The medium used for seed tank culture is: corn flour, soybean meal, glucose, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, magnesium sulfate and manganese sulfate.

[0042] 1. After the seed tank is checked correctly, add an appropriate amount of water, put in corn flour and soybean meal, raise the temperature in the seed tank to 43-45°C with a jacket, then add protease to liquefy for one hour, then add medium-temperature amylase, and heat up to 65- Liquified at 67°C for another hour.

[0043] The addition amount of protease is 10g, and the addition amount of mesophilic amylase is 6g;

[0044] 2. After the liquefaction is completed, add the remaining culture med...

Embodiment 2

[0063] Embodiment 2 A kind of Bacillus licheniformis fermentation culture technique

[0064] Including the seed tank culture stage and the fermenter culture stage.

[0065] The strain used was Bacillus licheniformis ACCC10146.

[0066] First, seed pot culture

[0067] Using a 100 liter seed tank:

[0068] The medium used for seed tank cultivation is: corn flour 11g / L, soybean meal 23g / L, glucose 4.6g / L, dipotassium hydrogen phosphate 1.7g / L, potassium dihydrogen phosphate 1.3g / L, magnesium sulfate 0.8g / L And manganese sulfate 0.5g / L.

[0069] 1. After the seed tank is checked correctly, add an appropriate amount of water, put in corn flour and soybean meal, raise the temperature in the seed tank to 47-49°C with a jacket, then add protease to liquefy for one hour, then add medium-temperature amylase, and heat up to 68- Liquified at 70°C for another hour.

[0070] The addition of protease is 12g, and the addition of mesophilic amylase is 7g;

[0071] 2. After the liquefact...

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Abstract

The invention provides a fermental cultivation process of bacillus licheniformis. The fermental cultivation process comprises the seed tank cultivation stage and the fermentation tank cultivation stage, specifically, the liquefying, sterilizing, inoculating and cultivating steps are included at the seed tank cultivation stage, and culture medium raw materials adopted for seed tank cultivation comprise corn flour, soybean meal, glucose, dipotassium phosphate, monopotassium phosphate, magnesium sulfate and manganese sulfate; and during fermental cultivation, the ventilation quantity in the firstfour hours is 1:(1 to 1.1), the later ventilation quantity is 1:(1.2 to 1.3), the cultivation temperature is 37+ / -0.5 DEG C, the stirring speed is 150-160 rpm in the first four hours and is 180-190 rpm after four hours, and the cultivation time is 30+ / -0.5 hours. According to the fermental cultivation process of the bacillus licheniformis, the gemma rate of the bacillus licheniformis reaches 98%or above.

Description

technical field [0001] The invention relates to a fermentation and cultivation process of Bacillus licheniformis, belonging to the technical field of microorganism cultivation. Background technique [0002] Bacillus licheniformis is a Gram-positive thermophilic bacterium commonly found in soil. The bacterium is also found in the feathers of birds, especially ground-dwelling birds such as finches and aquatic birds such as ducks, especially in the breast and back feathers. The optimum temperature for enzyme secretion is 37°C. It may exist in the form of spores to resist harsh environments; under favorable conditions, it can exist in a growing state. The bacteria can adjust the imbalance of flora to achieve the purpose of treatment, and can prompt the body to produce antibacterial active substances and kill pathogenic bacteria. It can produce anti-active substances, and has a unique mechanism of biological oxygen capture, which can inhibit the growth and reproduction of path...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/10
CPCC12N1/20Y02P60/87
Inventor 郭芳先韩威华陈永科刘镇王于玺肖发沂马爱霞
Owner SHANDONG SUKAHAN BIO TECH
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