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Preparation process of bacillus licheniformis[v]

The invention relates to a Bacillus licheniformis and preparation process technology, which is applied in the field of microbial science and technology, and can solve the problems of unstable quantity and quality of Bacillus licheniformis spores, large variation of Bacillus licheniformis coefficient, waste of Bacillus licheniformis raw materials, etc. The effect of high product quality and long shelf life

Pending Publication Date: 2020-05-26
南宁汉和生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the situation that the number and quality of spores in the preparation of Bacillus licheniformis are not stable due to the large changes in the coefficients of the preparation environment of Bacillus licheniformis and the unstable control of environmental parameters, so that Bacillus licheniformis can be produced during preparation. There are problems such as raw material waste and low yield, and a preparation process for Bacillus licheniformis is provided

Method used

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Embodiment Construction

[0035] This specific embodiment adopts the following technical scheme: a preparation process of Bacillus licheniformis, including seed cultivation and fermentation cultivation, the seed cultivation includes liquefaction and sterilization, and the fermentor cultivation includes inoculation and fermentation cultivation. The specific steps are:

[0036] 1), liquefaction: first liquefy the seeds in the seed tank;

[0037] 2), sterilization: then sterilize the liquefied seeds;

[0038] 3), inoculation: inoculate the sterilized seeds into the cultivation tank for preliminary cultivation;

[0039] 4) Fermentation: transfer the seeds in the cultivation tank to the fermentation tank for fermentation.

[0040] Wherein, the liquefaction step comprises:

[0041] 1), first take out the Bacillus licheniformis bacteria tube stored in the ultra-low temperature refrigerator, insert it into the slope with an inoculation loop under aseptic conditions, transfer it to the seed tank, and mix corn...

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Abstract

The invention discloses a preparation process of bacillus licheniformis. The method comprises seed culture and fermentation culture, wherein the seed culture comprises liquefaction and sterilization;the fermentation tank culture comprises inoculation and fermentation culture; the process steps are rigorous; through detailed description of each step and solidification of environmental parameters in each step, the occurrence probability of maldevelopment of bacillus in the fermentation cultivation process is reduced; the bacillus licheniformis fermentation technology guarantees that the numberand the quality of the fermented bacillus licheniformis are far higher than those of fermentation results in the prior art, the environment in the preparation process of the technology is a sterile environment, the infectious microbe rate in the produced product is low, the product quality is high, and the bacillus licheniformis prepared through the technology is long in quality guarantee period and can be stored for a long time.

Description

Technical field: [0001] The invention relates to the technical field of microbial technology, in particular to a preparation process of Bacillus licheniformis. Background technique: [0002] Bacillus licheniformis is a Gram-positive thermophilic bacterium commonly found in soil and also found in the feathers of birds, especially ground-dwelling and aquatic birds, especially on their In the feathers on the chest and back, the optimum temperature for enzyme secretion is 37°C. It may exist in the form of spores to resist harsh environments; in good environments, it can exist in a growth state, and the bacteria can adjust the imbalance of flora to achieve treatment Purpose, can promote the body to produce antibacterial active substances, kill pathogenic bacteria, can produce antibacterial active substances, and has a unique mechanism of biological oxygen capture, can inhibit the growth and reproduction of pathogenic bacteria, and has the ability to promote normal physiological a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/10
CPCC12N1/20
Inventor 梁承陈成邹仕刚邹林君吴宁闭革林吴志雄
Owner 南宁汉和生物科技股份有限公司
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