Preparation process of bacillus licheniformis[v]
The invention relates to a Bacillus licheniformis and preparation process technology, which is applied in the field of microbial science and technology, and can solve the problems of unstable quantity and quality of Bacillus licheniformis spores, large variation of Bacillus licheniformis coefficient, waste of Bacillus licheniformis raw materials, etc. The effect of high product quality and long shelf life
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[0035] This specific embodiment adopts the following technical scheme: a preparation process of Bacillus licheniformis, including seed cultivation and fermentation cultivation, the seed cultivation includes liquefaction and sterilization, and the fermentor cultivation includes inoculation and fermentation cultivation. The specific steps are:
[0036] 1), liquefaction: first liquefy the seeds in the seed tank;
[0037] 2), sterilization: then sterilize the liquefied seeds;
[0038] 3), inoculation: inoculate the sterilized seeds into the cultivation tank for preliminary cultivation;
[0039] 4) Fermentation: transfer the seeds in the cultivation tank to the fermentation tank for fermentation.
[0040] Wherein, the liquefaction step comprises:
[0041] 1), first take out the Bacillus licheniformis bacteria tube stored in the ultra-low temperature refrigerator, insert it into the slope with an inoculation loop under aseptic conditions, transfer it to the seed tank, and mix corn...
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