Method for producing viable bacteria of bacillus licheniformis by high-density solid fermentation

Abstract

The invention relates to the field of biotechnology, in particular to a method for producing viable bacillus licheniformis by high-density solid fermentation, which comprises the following steps: strain selection, slant seed cultivation, solid seed cultivation, solid fermentation cultivation, fermentation product drying and The fermentation product is flash evaporated. The raw materials of the culture medium of the present invention are all agricultural by-products, the cost is low, the formula is reasonable, and the nutritional requirements for the propagation of bacteria can be met. Especially adding the pH value change of medium in the calcium carbonate buffer culture process can greatly improve the effective viable bacteria content of bacillus licheniformis of solid fermentation, and experiment and production prove that the effective viable bacteria number of bacillus licheniformis prepared by the inventive method can reach Up to 100 billion / g, the rate of miscellaneous bacteria in the product is less than 5%, and the rate of spores is high, reaching more than 90%, which can greatly reduce the production cost of enterprises and meet the needs of microbial bacterial agent production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing live bacillus licheniformis by high-density solid fermentation. Background technique [0002] Bacillus licheniformis ( Baclicus lincheniformis ) is a type of Bacillus, single cell, 0.6~0.8×1.5~3 microns, cell shape and arrangement are rod-shaped, solitary, no poly-β-hydroxybutyrate particles in the cell, Gram-positive bacillus . Produce mesophilic ellipsoid spores with slightly enlarged cysts. The colonies are flat, irregularly bordered, and white. [0003] Bacillus licheniformis ( Baclicus lincheniformis ) has multiple functions and is an excellent strain that can be used in many fields, such as microbial pesticides, microbial feed additives, microbial fertilizers, water purifiers, etc. Its main function is to inhibit the growth of harmful bacteria (Escherichia coli, Clostridium perfringens), increase the beneficial bacteria in the body and reduce the pot...

AI Technical Summary

Problems solved by technology

[0004] The production of Bacillus licheniformis mainly includes liquid fermentation and solid fermentation. Liquid fermentation requires a complete set of fermenter equipment. Its advantages are that the fermentation process is easy to control and the product has high purity. The disadvantage is that it needs to be adsorbed and dried after fermentation. High energy consumption and serious environmental pollution; solid fermentation is generally carried out in the fermentation room. Bacterial contamination is difficult to control

[0005] The Chinese patent application number is 201110324147.2, which discloses a method for producing live bacteria of Bacillus subtilis by solid fermentation, but the strains used, the specific method of seed cultivation, the specific operation steps of solid fermentation cultivation, and the fermentation products are air-dried. The drying method and the formula of the medium used are different, and the operation method of the reference document is complicated and the production efficiency is low

Due to the heat generated during the fermentation process, the temperature of the material rises rapidly, and the water and temperature are not easy to control in the general open culture method.