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Microbial agent, organic waste sandy soil remediation agent and its preparation method and application

A technology of microbial inoculum and soil remediation agent, applied in the field of microbial inoculum, can solve the problems of fast expansion, severe development situation, decline of land productivity, etc., and achieve the effects of short fermentation period, improved agricultural product quality, and increased water retention capacity.

Active Publication Date: 2022-01-14
北京绿安创华环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Second, the expansion speed is fast and the development situation is severe
leading to a severe decline in land productivity

Method used

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  • Microbial agent, organic waste sandy soil remediation agent and its preparation method and application
  • Microbial agent, organic waste sandy soil remediation agent and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Present embodiment provides the microbial inoculant containing Trichoderma aculeatus spore powder and preparation method thereof, and the method comprises the steps:

[0051] 1) Connect the preserved strains to a PDA medium plate, activate and culture at 28°C until sporulation, and sieve the obtained spore powder (200 mesh) to obtain the Trichoderma aculeatus spore powder;

[0052] Take the spore powder and make it into a concentration of 1×10 11 spore / mL suspension to obtain the first microbial liquid inoculant.

[0053] Wherein, the configuration method of the PDA medium mentioned above is as follows: Weigh 200g of potatoes, wash, peel and cut into small pieces, add 1000ml of water and boil for half an hour, filter with gauze, add 10-20g of glucose, after fully dissolving Dilute to 1000ml and sterilize at 121°C for 30min.

[0054] 2) Take the strain of Bacillus stearothermophilus, pick a ring of the strain and insert it into a 100ml shaker flask containing 15ml of s...

Embodiment 2

[0060] In this example, the preparation operation is the same as in Example 1. The difference from Example 1 is that in this example, in addition to adding Bacillus stearothermophilus and Acremonium acremonium, Lactobacillus plantarum, Li Trichoderma, Lactobacillus delbrueckii, Micrococcus methanophilus, Thermoanaerobacillus metalophilus, Actinomyces albicans, the above strains are composed according to the ratio of 3:3:7:7:3:3:3 After adding the compound bacteria solution, the total amount of each bacteria added was 1×10 11 CFU / mL.

Embodiment 3

[0062] This embodiment provides a solid bacterial agent and a preparation method thereof, specifically comprising the following steps:

[0063] Add the liquid bacterial agent prepared in the above-mentioned Example 2 into the talcum powder according to the ratio of 20 grams of talc powder (as a solid carrier) per 1 kg of liquid bacterial agent, stir and mix well, filter, dry, and pulverize to obtain solid bacterial agent agent.

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Abstract

The invention relates to a microbial bacterial agent, which contains Trichoderma aculeatus WL, live spores of Bacillus stearothermophilus and Claudium acremonium. Composting organic garbage by using the compound microbial bacterial agent is beneficial to improving the maturity of materials, and has a complexing effect on heavy metal ions to change the state of heavy metal ions and has strong tolerance. The present invention also relates to a sandy soil remediation agent for organic waste, which is obtained by mixing fermentation products and organic waste preparations; Obtained; the preparation method of the organic waste preparation comprises: the organic waste is soaked in water for 10-14 hours after removing impurities and crushing, stirring into a paste under the environment of pH 9-10, and then adding vinyl monomers to carry out graft copolymerization reaction . The soil remediation agent solves the problem of land desertification and remediates soil polluted by heavy metals.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a microbial bacterial agent, an organic garbage desertification soil remediation agent and a preparation method and application thereof. Background technique [0002] 43% of the earth's surface is distributed in arid regions, and more than 110 countries suffer from desertification, which causes a huge loss of 82 billion U.S. dollars every year in the world. my country has a large area and wide distribution. The national desertification land area reaches 1.743 million square kilometers, accounting for 18% of the country's land area, involving 841 counties (banners) in 30 provinces (regions, cities) across the country. The eight major deserts and the four major sandy lands are the main sand sources in my country, and there are sporadic sandy lands along the rivers, rivers, and seas in the south. The national area of ​​mobile sand dunes is 427,200 square kilometers, f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/20B09C1/10C05G3/80C12R1/885C12R1/07C12R1/645
CPCC12N1/14C12N1/20C05B7/00B09C1/10B09C1/105C05G3/80C12R2001/885C12N1/145C05C3/00C05C11/00C05D3/00C05D9/00C05D9/02C05F3/04C05F7/00C05F9/00C05F11/00C05F11/04C05F11/08
Inventor 王锋
Owner 北京绿安创华环保科技有限公司
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