Method for producing bacillus laterosporus live bacteria by high density solid fermentation

Abstract

The invention relates to the biotechnical field, and in particular relates to a method for producing bacillus laterosporus live bacteria by high density solid fermentation. The method comprises the following steps: culture selection; bevel seed cultivation; primary solid seed cultivation; secondary solid seed cultivation; solid fermentation and cultivation; fermentation product drying; and fermentation product flash evaporation. Raw materials of the culture media provided by the invention are subsidiary agricultural products which are low in cost and reasonable in formula and can meet the nutritional demand of strain reproduction. The pH value of the culture media in the process of cultivation by adding a calcium carbonate buffer is in particularly changed, so that the content of active bacteria of bacillus laterosporus by solid fermentation is greatly improved. Experiments and production show that the number of effective active bacteria of bacillus laterosporus prepared by the method can reach 50 billion / g, the percentage of contaminating microorganisms of the product is lower than 5% and the spore rate reaches over 90%, so that the production coast of enterprises is greatly lowered, and the demand of production of microbial agents is satisfied.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing live Bacillus lateralosporus by high-density solid fermentation. Background technique [0002] Bacillus lateralis ( Bacillus laterosporus ) is a kind of Bacillus genus, the thallus is rod-shaped, the end is slightly thinner, and the solitary forms pairs and piles; the spores are oval, with a size of (1~1.2)×(2.5~3) microns, attached to one side of the spore It is in the shape of a canoe, and the spores grow laterally in the spindle-shaped cysts. [0003] Bacillus lateralis can promote the growth of beneficial bacteria in plant roots, inhibit the reproduction of pathogenic bacteria, promote the growth of plant roots, enhance the absorption capacity of roots, and activate soil nutrients, thereby increasing crop yield and quality. It is an extremely effective microbial fertilizer. At the same time, Bacillus lateralosporus can also be used as a micro-ecological f...

AI Technical Summary

Problems solved by technology

[0005] The production of Bacillus lateralis mainly includes liquid fermentation and solid fermentation. Liquid fermentation requires a complete set of fermenter equipment. Its advantages are that the fermentation process is easy to control and the product has high purity. The disadvantage is that it needs to be adsorbed and dried after fermentation. There are many process procedures and large investment. , high energy consumption, serious environmental pollution, and high requirements for personnel and equipment; solid fermentation is generally carried out in the fermentation room, which has the advantages of high fermentation output and no need for adsorption after fermentation, it can be used directly by drying and crushing, and the process is simple , less investment, the disadvantage is that the fermentation process is not easy to control, and the contamination of bacteria is not easy to control

[0006] The Chinese patent application number is 201110324147.2, which discloses a method for producing live bacteria of Bacillus subtilis by solid fermentation, but the strains used, the specific method of seed cultivation, the specific operation steps of solid fermentation cultivation, and the fermentation products are air-dried. The drying method and the formula of the medium used are not the same. The operation method of the reference file is complicated and the production efficiency is low

Due to the heat generated during the fermentation process, the temperature of the material rises rapidly, and the water and temperature are not easy to control in the general open culture method.