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Method for preparing trichoderma harzianum high-activity conidial powder

A technology of active spores and spores, which is applied in the field of Trichoderma harzianum chlamydospore fermentation technology and the preparation of spore powder with high viable counts, can solve the problems of weakening market competitiveness, long drying time, and increased costs, and achieve rapid adaptation to the soil environment , long shelf life and low cost

Inactive Publication Date: 2019-03-29
SICHUAN LANYUE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These collection methods have their own advantages and disadvantages. If the first method is adopted, the total number of drying and drying of the culture is n, and the number of spores finally obtained is reduced by 10 n times compared with the initial culture; if the second method is adopted , although the spore survival rate of the product is guaranteed, the process is cumbersome, the drying time is long, the energy consumption is high, and the cost is greatly increased, which weakens the market competitiveness of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Prepare the first-class species:

[0027] (1) Preparation of PDA plates: take 40g of peeled potatoes and boil for 30min, filter to obtain potato juice, add 4g of glucose, 3.6g of agar, add water and dissolve to 200mL, sterilize at 121°C for 30min, after sterilization, pour the pre-sterilized Bacteria in a Petri dish, cooled for later use;

[0028] (2) Preparation of the primary species: Inoculate Trichoderma harzianum on a PDA plate under aseptic conditions and culture at 30°C for 5 days.

[0029] 2. Preparation of secondary species:

[0030] (1) Culture medium preparation: Take 70g of oat flour, 1.8g of sucrose, 1.8g of dipotassium hydrogen phosphate, 2.1g of magnesium sulfate, add water to 3.5L, and sterilize at 121°C for 20 minutes;

[0031] (2) Secondary seed preparation: Under aseptic conditions, use a puncher with a hole diameter of 6mm to punch holes on the prepared primary seed plate, and inoculate the prepared medium with an inoculation amount of 7 pieces / ...

Embodiment 2

[0044] 1. Prepare the first-class species:

[0045] (1) Prepare Martin's plate: Take 1g peptone, 2g glucose, 0.2g potassium dihydrogen phosphate, 0.1g magnesium sulfate, 0.66mL 1% Bengal red aqueous solution, 3.6g agar, add water to 200mL, and sterilize at 121°C for 20min , after sterilization, pour it into a petri dish that has been sterilized in advance, and cool it for later use;

[0046] (2) Preparation of the primary species: Inoculate Trichoderma harzianum on a Martin plate under aseptic conditions, and culture at 28°C for 5 days.

[0047] 2. Preparation of secondary species:

[0048] (1) Culture medium preparation: Take 78g oat flour, 1.4g sucrose, 1.6g dipotassium hydrogen phosphate, 6g magnesium sulfate, add water to 2L, and sterilize at 121°C for 30min;

[0049] (2) Secondary seed preparation: Under aseptic conditions, use a hole puncher with a hole diameter of 10mm to punch holes on the prepared primary seed plate, and inoculate the prepared medium with an inocula...

Embodiment 3

[0062] 1. Prepare the first-class species:

[0063] (1) Prepare Martin's plate: Take 1g peptone, 2g glucose, 0.2g potassium dihydrogen phosphate, 0.1g magnesium sulfate, 0.66mL 1% Bengal red aqueous solution, 3.6g agar, add water to 200mL, and sterilize at 121°C for 20min , after sterilization, pour it into a petri dish that has been sterilized in advance, and cool it for later use;

[0064] (2) Preparation of the primary species: Inoculate Trichoderma harzianum on a Martin plate under aseptic conditions, and culture at 28°C for 5 days.

[0065] 2. Preparation of secondary species:

[0066] (1) Culture medium preparation: Take 9g oat flour, 7g sucrose, 0.35g dipotassium hydrogen phosphate, 0.04g magnesium sulfate, add water to 0.7L, and sterilize at 121°C for 30min;

[0067] (2) Secondary seed preparation: Under aseptic conditions, use a puncher with a hole diameter of 6 mm to punch holes on the prepared primary seed plate, and inoculate the prepared medium with an inoculati...

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Abstract

The invention provides a method for preparing trichoderma harzianum high-activity conidial powder. The method comprises the following steps: performing step-by-step propagating fermentation on a trichoderma harzianum strain, from preparation production of stock culture, mother spawn and spawn to production of high-concentration trichoderma harzianum chlamydospore fermentation liquor by adopting astepwise liquid fermentation method; and concentrating the fermentation liquor, adding different functional additives and filler into the concentrated fermentation liquor, and performing drying treatment to obtain the trichoderma harzianum high-activity conidial powder. The trichoderma harzianum chlamydospore has the number of effective living bacteria being 2*10<9>cfu / g or more, and has the advantages of high activity, long shelf life, high germination rate, quick soil environment adaptability and the like.

Description

technical field [0001] The invention relates to a Trichoderma harzianum chlamydospore fermentation technology and a method for preparing spore powder with high viable count. Background technique [0002] Trichoderma harzianum has a variety of biological control and growth-promoting properties. It has good effects, environmental protection and safety, and is more and more popular in the market. The Trichoderma harzianum spores have the characteristics of storage resistance, convenient transportation, and easy application. Therefore, in order to realize the commercialization of Trichoderma harzianum, the preparation of high-concentration, high-activity, and low-cost Trichoderma harzianum spore powder has become the focus of formulation development. [0003] Trichoderma harzianum has two spore forms, one is the conidia produced on the side branches of the mycelium, and the other is the chlamydospore directly from the division of the mycelium. At present, most of the commercial...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12N1/14C12N1/04C12R1/885
CPCC12N1/04C12N1/14C12N3/00
Inventor 朱彭玲侯勇王强锋夏中梅刘武王贇陈春许艳曾显斌
Owner SICHUAN LANYUE SCI & TECH
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